Rat PDGF-AB ELISA Kit PicoKine®

Pdgfb ELISA kit for Rat

Rat PDGF-AB ELISA Kit PicoKine® (96 Tests). Quantitate Rat PDGF-AB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Info Summary

SKU: EK0485
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Rat PDGF-AB ELISA Kit PicoKine®

View all Pdgfb ELISA kits

SKU/Catalog Number

EK0485

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat PDGF-AB ELISA Kit PicoKine® (96 Tests). Quantitate Rat PDGF-AB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Rat PDGF-AB ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0485)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: (R86-K204) + (S74-T182)

Sensitivity

<3 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of EK0485 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0485 is reactive to PDGF-AB in Rat samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0485 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Pdgfb

The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains. The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines. Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281. The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner. PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23. The human homolog (PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22. The standard product used in this kit is recombinant rat PDGF-AB (A chain Ser87-Arg196; B chain Ser74-Thr182) with the molecular mass of 24.8KDa.

Kit Components

Catalog Number Description Quantity
EK0485-CAP Anti-Rat PDGF-AB Pre-coated 96-well strip microplate 1
EK0485-ST Rat PDGF-AB Standard 2 vials, 10 ng/tube
EK0485-DA Rat PDGF-AB Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0220.0970.1350.2410.4700.8321.3491.833

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Pdgfb, For serum, dilution ratio is 1:5 and concentration is around 1.5 ng/ml.
For plasma, dilution ratio is 1:1 and concentration is 0-46 pg/ml..

Some articles we found to cite concentrations of Pdgfb in samples: 17706067 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat PDGF-AB ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)8628288583277930
Standard deviation4.914.3858.414.9816.8974.4
CV (%)5.7%8%%6.6%6%6.1%8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Pdgfb in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 186838586851.221.4%
Sample 22822772832822812.340.8%
Sample 388583485788886622.072.5%
*number of samples for each test n=16.

Gene/Protein Information For PDGF-AB (Source: Uniprot.Org, NCBI)

Gene Name

PDGF-AB

Full Name

Platelet-derived growth factor subunit B

Weight

27422 MW

Superfamily

PDGF/VEGF growth factor family

Alternative Names

Platelet derived growth factor, B polypeptide ;Platelet-derived growth factor subunit B ;Pdgfb ;rCG_59494 ; Pdgfb|SIS, c-sis|platelet derived growth factor subunit B|platelet-derived growth factor subunit B|PDGF subunit B|PDGF-2|Platelet-derived growth factor, same as Sis (Simian sarcoma viral oncogene homologue, c-sis)|pdgf protein|platelet derived growth factor, B polypeptide|platelet derived growth factor-bb|platelet-derived growth factor B chain|platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PDGF-AB, check out the PDGF-AB Infographic

PDGF-AB infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PDGF-AB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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5 Customer Q&As for Rat PDGF-AB ELISA Kit PicoKine®

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-01-08

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-01-08

Question

Q: how can I thaw whole blood sample for PDGFB ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-07-21

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test PDGFB for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2018-07-21

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement PDGFB using the EK0485 Rat PDGFB Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-01-18

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2018-01-18

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2016-11-14

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-11-14

Question

Q: if the enzyme conjugated PDGFB antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the PDGFB antigen?

Verified Customer

Verified customer

Asked: 2014-06-13

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2014-06-13

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