Overview
| Product Name |
Rat PDGF-AB ELISA Kit PicoKine™
|
| Catalog# |
EK0485 |
| Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Description |
Rat PDGF-AB ELISA Kit PicoKine™ (96 Tests). Quantitate Rat PDGFB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 3pg/ml. |
| Cite This Product |
Rat PDGF-AB ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0485)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Rat |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity |
<3 pg/ml |
| Assay Range |
31.2 pg/ml - 2,000 pg/ml |
| Immunogen/Standard |
Expression system for standard: E.coli; Immunogen sequence: (R86-K204) + (S74-T182) |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For PDGFB (Source: Uniprot.org, NCBI)
| Uniprot Id | G3V882 |
|---|
| Gene Name | PDGFB |
|---|
| Molecular Weight | 27422 MW |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on PDGFB, check out the PDGFB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for PDGFB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the PDGFB infographic
Kit Components And Assay QC Data Details Of Rat PDGF-AB ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0485-CAP |
Anti-Rat PDGFB Pre-coated 96-well strip microplate | 1 |
| EK0485-ST | Rat PDGFB Standard | 2 vials, 10 ng/tube |
| EK0485-DA | Rat PDGFB Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0485 Rat PDGF-AB ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.022 | 0.097 | 0.135 | 0.241 | 0.470 | 0.832 | 1.349 | 1.833 |
We measured random samples of Rat PDGF-AB ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 86 | 282 | 885 | 83 | 277 | 930 |
| Standard deviation | 4.9 | 14.38 | 58.41 | 4.98 | 16.89 | 74.4 |
| CV (%) | 5.7% | 8%% | 6.6% | 6% | 6.1% | 8% |
We measured the performance consistency of Rat PDGF-AB ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 86 | 83 | 85 | 86 | 85 | 1.22 | 1.4% |
| Sample 2 | 282 | 277 | 283 | 282 | 281 | 2.34 | 0.8% |
| Sample 3 | 885 | 834 | 857 | 888 | 866 | 22.07 | 2.5% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 0
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Questions and answers from customers related to EK0485 Rat PDGF-AB ELISA Kit PicoKine™
5 Related Questions
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Asked: 2019-01-08
Boster Scientific Support
Answered: 2019-01-08
Question
Q: how can I thaw whole blood sample for PDGFB ELISA after freezing?
Verified Customer
Asked: 2018-07-21
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test PDGFB for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-07-21
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement PDGFB using the EK0485 Rat PDGFB Picokine® ELISA Kit?
Verified Customer
Asked: 2018-01-18
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2018-01-18
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Asked: 2016-11-14
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-11-14
Question
Q: if the enzyme conjugated PDGFB antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the PDGFB antigen?
Verified Customer
Asked: 2014-06-13
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2014-06-13
