Overview
| Product Name |
Rat TIMP-2 ELISA Kit PicoKine™
See all TIMP-2 primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
EK1302 |
| Storage & Handling |
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description |
Detect Rat TIMP2 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. TIMP2 tissue specificity: |
| Cite This Product |
Rat TIMP-2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1302)
|
Product Specs
| Reactivity/Species |
Rat |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity |
<10pg/ml |
| Assay Range |
156pg/ml-10000pg/ml |
| Immunogen/Standard |
Expression system for standard: NSO; Immunogen sequence: C27-P220 |
| Antibody Clonalities |
Capture antibody|Detection antibody
polyclonal antibody from goat|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96wells/kit, with removable strips. |
Gene/Protein Basic Information For Timp2 (Source: Uniprot.org, NCBI)
| Uniprot Id | P30121 |
|---|
| NCBI Gene Id | 29543 |
|---|
| Species Of This Entry | Rat |
|---|
| Gene Name | Timp2 |
|---|
| Protein Name | Metalloproteinase inhibitor 2 |
|---|
| Superfamily | protease inhibitor I35 (TIMP) family |
|---|
| Alternative Names | TIMP-2|CSC-21Ktissue inhibitor of metalloproteinase 2; metalloproteinase inhibitor 2; TIMP metallopeptidase inhibitor 2; TIMP2; TIMP-2; Tissue inhibitor of metalloproteinases 2 |
|---|
| Molecular Weight | 24356 |
|---|
| Post Tranlational Modifications | Phosphorylation; Cleavage; Methylation; Demethylation; Reduction; Acetylation; Dephosphorylation; Oxidation; Ribosylation |
|---|
| Gene Location | 10q32.3, on Chromasome 10, gene sequence: |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Timp2 (Source: Uniprot.org, NCBI)
| Protein Function | Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. |
|---|
| Related Diseases | Neoplasms; Malignant Neoplasms; Cell Invasion; Neoplasm Metastasis; Carcinoma; Neoplasm Invasiveness; Fibrosis; Tumor Angiogenesis; Malignant Paraganglionic Neoplasm; Inflammation; Tissue Adhesions; Pathologic Neovascularization; Adenocarcinoma; Cardiovascular Diseases; Endometrial Polyp; Kidney Diseases; Lung Neoplasms; Malignant Neoplasm Of Breast; Liver Neoplasms |
|---|
| Related Pathways | Angiogenesis; Pathogenesis; Localization; Cell Migration; Cell Proliferation; Secretion; Tissue Remodeling; Proteolysis; Reverse Transcription; Cell Adhesion; Methylation; Cell Growth; Cell Motility; Dna Methylation; Wound Healing; Regeneration; Cell Cycle; Transport; Invasive Growth; Cell Death |
|---|
| Background | TIMP-2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP-2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid. |
|---|
| Scientific References | PMID: 8203893 by Cook T.F., et al. Cloning and regulation of rat tissue inhibitor of metalloproteinases- 2 in osteoblastic cells.
PMID: 8050496 by Santoro M., et al. Cloning of the rat tissue inhibitor of metalloproteinases type 2 (TIMP-2) gene: analysis of its expression in normal and transformed thyroid cells. |
|---|
Kit Components And Assay QC Data Details Of Rat TIMP-2 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK1302-CAP | 96-well plate precoated with anti-Rat TIMP-2 antibody | 1 |
| EK1302-ST | lyophilized recombinant Rat TIMP-2 standard | 10ng/tube |
| EK1302-DA | biotinylated anti-Rat TIMP-2 antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK1302 Rat TIMP-2 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.028 | 0.118 | 0.205 | 0.402 | 0.849 | 1.345 | 1.970 | 2.236 |
We measured random samples of Rat TIMP-2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 240 | 1342 | 4533 | 227 | 1463 | 4769 |
| Standard deviation | 10.56 | 68.44 | 339.97 | 10.21 | 95.09 | 443.51 |
| CV(%) | 4.4% | 9.3% | 7.5% | 4.5% | 6.5% | 9.3% |
We measured the performance consistency of Rat TIMP-2 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 240 | 251 | 238 | 213 | 235 | 13.9 | 5.9% |
| Sample 2 | 1342 | 1286 | 1409 | 1375 | 1353 | 45.35 | 3.3% |
| Sample 3 | 4533 | 4407 | 4200 | 4479 | 4404 | 126.38 | 2.8% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all TIMP-2 primary antibodies,
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 6
| Kim Ks, Lee Ya, Choi Hm, Yoo Mc, Yang Hi. Rheumatol Int. 2012 Oct;32(10):3069-75. Epub 2011 Sep 13. Implication Of Mmp-9 And Urokinase Plasminogen Activator (Upa) In The Activation Of Pro-Matrix Metalloproteinase (Mmp)-13. |
PubMed ID 21913037 |
| Gishto A, Farrell K, Kothapalli Cr. J Biomed Mater Res A. 2015 Feb;103(2):693-708. Doi: 10.1002/Jbm.A.35217. Epub 2014 May 19. Tuning Composition And Architecture Of Biomimetic Scaffolds For Enhanced Matrix Synthesis By Murine Cardiomyocytes. |
PubMed ID 24798055 |
| Yu X, Zhu J, Mi M, Chen W, Pan Q, Wei M. Med Oncol. 2012 Mar;29(1):349-57. Doi: 10.1007/S12032-010-9770-2. Epub 2010 Dec 4. Anti-Angiogenic Genistein Inhibits Vegf-Induced Endothelial Cell Activation By Decreasing Ptk Activity And Mapk Activation. |
PubMed ID 21132400 |
| Xu Xl, Ling Dy, Zhu Qy, Fan Wj, Zhang W. Eur J Pharmacol. 2013 Jan 5;698(1-3):370-8. Doi: 10.1016/J.Ejphar.2012.11.019. Epub 2012 Nov 21. The Effect Of 2,3,4',5-Tetrahydroxystilbene-2-0-??-D Glucoside On Neointima Formation In A Rat Artery Balloon... |
PubMed ID 23178522 |
| Cavdar Z, Ozbal S, Celik A, Ergur Bu, Guneli E, Ural C, Camsari T, Guner Ga. Biotech Histochem. 2014 May;89(4):304-14. Doi: 10.3109/10520295.2013.847498. Epub 2013 Oct 28. The Effects Of Alpha-Lipoic Acid On Mmp-2 And Mmp-9 Activities In A Rat Ren... |
PubMed ID 24160412 |
| Zhang, B., Shi, Y.Q., Zou, J.J., Chen, X.F., Tang, W., Ye, F., & Liu, Z.M. (2017). High glucose stimulates cell proliferation and Collagen IV production in rat mesangial cells through inhibiting AMPK-KATP signaling. International Urology and Nephr... |
PubMed ID 28748494 |
