Overview
Product Name |
Rat TIMP-2 ELISA Kit PicoKine™
See more TIMP-2 products |
Catalog# |
EK1302 |
Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Description |
Rat TIMP-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Timp2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 10pg/ml. |
Cite This Product |
Rat TIMP-2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1302)
|
ELISA Validation |
ELISA Validation Information
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Product Specs
Reactivity/Species |
Rat |
Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
Sample Type |
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Sensitivity |
<10 pg/ml |
Assay Range |
156 pg/ml - 10,000 pg/ml |
Immunogen/Standard |
Expression system for standard: NS0; Immunogen sequence: C27-P220 |
Antibody Clonalities |
Capture antibody|Detection antibody polyclonal antibody from goat|polyclonal antibody from goat |
Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For Timp2 (Source: Uniprot.org, NCBI)
Uniprot Id | P30121 |
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NCBI Gene Id | 29543 |
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Species Of This Entry | Rat |
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Gene Name | Timp2 |
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Protein Name | Metalloproteinase inhibitor 2 |
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Superfamily | protease inhibitor I35 (TIMP) family |
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Alternative Names | TIMP-2|CSC-21Ktissue inhibitor of metalloproteinase 2; metalloproteinase inhibitor 2; TIMP metallopeptidase inhibitor 2; TIMP2; TIMP-2; Tissue inhibitor of metalloproteinases 2 |
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Molecular Weight | 24356 |
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*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Timp2, check out the Timp2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Timp2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the Timp2 infographic
Kit Components And Assay QC Data Details Of Rat TIMP-2 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
Catalog number | Description | Quantity |
EK1302-CAP |
Anti-Rat Timp2 Pre-coated 96-well strip microplate | 1 |
EK1302-ST | Rat Timp2 Standard | 2 vials, 10 ng/tube |
EK1302-DA | Rat Timp2 Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK1302 Rat TIMP-2 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.028 | 0.118 | 0.205 | 0.402 | 0.849 | 1.345 | 1.970 | 2.236 |
We measured random samples of Rat TIMP-2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
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Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 240 | 1342 | 4533 | 227 | 1463 | 4769 |
Standard deviation | 10.56 | 68.44 | 339.97 | 10.21 | 95.09 | 443.51 |
CV (%) | 4.4% | 9.3% | 7.5% | 4.5% | 6.5% | 9.3% |
We measured the performance consistency of Rat TIMP-2 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
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Sample 1 | 240 | 251 | 238 | 213 | 235 | 13.9 | 5.9% |
Sample 2 | 1342 | 1286 | 1409 | 1375 | 1353 | 45.35 | 3.3% |
Sample 3 | 4533 | 4407 | 4200 | 4479 | 4404 | 126.38 | 2.8% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
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Total number of citations: 6
Zhang, B., Shi, Y.Q., Zou, J.J., Chen, X.F., Tang, W., Ye, F., & Liu, Z.M. (2017). High glucose stimulates cell proliferation and Collagen IV production in rat mesangial cells through inhibiting AMPK-KATP signaling. International Urology and Nephr... |
PubMed ID 28748494 |
Cavdar Z, Ozbal S, Celik A, Ergur Bu, Guneli E, Ural C, Camsari T, Guner Ga. Biotech Histochem. 2014 May;89(4):304-14. Doi: 10.3109/10520295.2013.847498. Epub 2013 Oct 28. The Effects Of Alpha-Lipoic Acid On Mmp-2 And Mmp-9 Activities In A Rat Ren... |
PubMed ID 24160412 |
Xu Xl, Ling Dy, Zhu Qy, Fan Wj, Zhang W. Eur J Pharmacol. 2013 Jan 5;698(1-3):370-8. Doi: 10.1016/J.Ejphar.2012.11.019. Epub 2012 Nov 21. The Effect Of 2,3,4',5-Tetrahydroxystilbene-2-0-??-D Glucoside On Neointima Formation In A Rat Artery Balloon... |
PubMed ID 23178522 |
Yu X, Zhu J, Mi M, Chen W, Pan Q, Wei M. Med Oncol. 2012 Mar;29(1):349-57. Doi: 10.1007/S12032-010-9770-2. Epub 2010 Dec 4. Anti-Angiogenic Genistein Inhibits Vegf-Induced Endothelial Cell Activation By Decreasing Ptk Activity And Mapk Activation. |
PubMed ID 21132400 |
Gishto A, Farrell K, Kothapalli Cr. J Biomed Mater Res A. 2015 Feb;103(2):693-708. Doi: 10.1002/Jbm.A.35217. Epub 2014 May 19. Tuning Composition And Architecture Of Biomimetic Scaffolds For Enhanced Matrix Synthesis By Murine Cardiomyocytes. |
PubMed ID 24798055 |
Kim Ks, Lee Ya, Choi Hm, Yoo Mc, Yang Hi. Rheumatol Int. 2012 Oct;32(10):3069-75. Epub 2011 Sep 13. Implication Of Mmp-9 And Urokinase Plasminogen Activator (Upa) In The Activation Of Pro-Matrix Metalloproteinase (Mmp)-13. |
PubMed ID 21913037 |
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK1302 Rat TIMP-2 ELISA Kit PicoKine™
7 Related Questions
Question
Q: What is the optimal O.D. value for TIMP-2 ELISA kit? I used your TIMP-2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain TIMP-2 even though the O.D. values are not very high?
Verified Customer
Asked: 2020-05-24
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TIMP-2 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of TIMP-2 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-05-24
Question
Q: is it okay to use citrate plasma as samples in Rat TIMP-2 Picokine® ELISA Kit (Catalog # EK1302)?
G. Zhang
Asked: 2019-10-23
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of TIMP-2 causing degradation of its protein structure. TIMP-2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the TIMP-2 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-10-23
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
D. Kumar
Asked: 2019-03-01
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-03-01
Question
Q: if the enzyme conjugated TIMP-2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TIMP-2 antigen?
Verified Customer
Asked: 2019-02-28
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-02-28
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of TIMP-2 in Rat serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Asked: 2018-03-09
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TIMP-2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-03-09
Question
Why are there precipitates in the samples diluent buffer for EK1302?
Answer
For the Rat TIMP-2 ELISA Kit PicoKine™ (EK1302), the precipitates are fatty substances contained in BSA.
It would not affect the performace of the kit.
Boster Scientific Support
Answered: 2019-05-30
Question
Have you tested EK1302 on urine samples? In your opinion, it would work?
Answer
We have not validated Rat TIMP-2 ELISA Kit PicoKine™ EK1302 with urine samples.
In theory, the ELISA kit will work for all sample types if the target protein is present at a level that falls within the linear range of the ELISA kit detection range. We guarantee the kit to work on the sample types that we have tested. For sample types that we have not tested for, we suggest your customer run pilot experiments to decide the optimal sample dilution.
Boster Scientific Support
Answered: 2019-03-01