RIPA Lysis Buffer

RIPA Lysis Buffer reagent is a complete cell lysis reagent popularly used for cultured mammalian cells. RIPA lysis buffer is highly compatible with immunoassays, protein purification procedures, immunoprecipitation, and western blotting. Cited in 73 publication(s).

Product Info Summary

SKU AR0105-100
Product name RIPA Lysis Buffer
Pack size 100ml
Form Solution (liquid)
Applications Western blotting, ELISA, immunofluorescence, Immunoprecipitation; protein purification; SDS-PAGE; protein assays; epitope tagging; reporter gene analysis

Overview

Form Supplied Ready-to-use 1X solution
Physical State Liquid
Pack Size 100 mL
Content 50mM Tris•HCl pH 7.6, 150mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS
Recommended working concentration 10 mL RIPA Lysis Buffer per gram of tissue
0.5 mL RIPA Lysis Buffer per 5.0x106 cells in suspension
0.5 mL RIPA Lysis Buffer per 5.0x106 adherent mammalian cells
Storage & Expiration Upon receipt store at 4°C. RIPA Lysis Buffer is stable for one year. Product is shipped on ice.
Assays per kit 200 assays for 5.0x106 cells
100 assays for 0.1g tissue
Compatibility with reagents Fully compatible with Broad Spectrum Protease Inhibitor Cocktail and Broad Spectrum Phosphatase Inhibitor Cocktail
Equivalent Thermofisher (Product No. 89900, 89901), Millipore Sigma (Product No. R0278)
Reagent Type Western Blotting related reagent; Cell lysis buffer; Universal tissue extraction buffer; Detergent solution
Usage Extraction of cytoplasmic, membrane and nuclear proteins
Cite This Product RIPA Lysis Buffer (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR0105-100)

Description

RIPA Lysis Buffer is a complete cell lysis solution reagent used for rapid and efficient total cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells, effectively extracting cytoplasmic, nuclear and membrane proteins. Protein lysis can be finished in 60 minutes.

Background

RIPA lysis extraction buffer contains non-ionic and ionic detergents which are able to extract protein from wide variety of cell types and membrane structures. RIPA buffer ensures efficient cell lysis and protein solubilization preventing protein degradation and interference with protein immunoreactivity and biological activity. Since most antibodies and protein antigens are not adversely affected by the components of this solution, RIPA buffer-conducted protein extraction is compatible with various downstream immunoprecipitation and molecular pull-down assays, including reporter assays, protein assays, immunoassays and protein purification. RIPA buffer reagent minimizes non-specific protein-binding interactions to keep background low, while allowing most specific interactions to occur, enabling studies of relevant protein-protein interactions.

Important Product Information

• If desired, add protease inhibitor (Product No. AR1182) and phosphatase inhibitor (Product No. AR1183) to the lysis buffer to prevent proteolysis and maintain phosphorylation status of proteins.

• Some protein kinases and other enzymes may be sensitive to the components of the RIPA Lysis Buffer, resulting in their decreased activity. In such cases, prepare a RIPA Lysis Buffer that does not contain sodium deoxycholate and SDS.

Additional Materials Required

• Protease inhibitor (Product No. AR1182) and phosphatase inhibitor (Product No. AR1183)

• 2 ml microcentrifuge tubes

• Tissue homogenizer

• Microcentrifuge capable of spinning at 10,000 x g

• Cell scraper

Procedure for Lysis of Monolayer-cultured Adherent Mammalian Cells

Note: Pre-chill an appropriate volume of RIPA Lysis Buffer at 4°C. If desired, add protease inhibitor and phosphatase inhibitor to the lysis buffer immediately before use.

1. In a microcentrifuge tube, resuspend 5×106 cells in the growth media by scraping the cells off the surface of the plate with a cell scraper. Centrifuge harvested cell suspension at 600xg for 5min, then carefully remove and discard the supernatant.

2. Resuspend the cells in chilled PBS. Centrifuge at 600xg for 5min, then carefully remove and discard the supernatant.

3. Add 0.5 mL of chilled RIPA lysis buffer to the cell pellet. Vortex briefly. Incubate on ice for 30 minutes.

4. Centrifuge samples at 14000xg for 10 minutes.

5. Transfer supernatant to a new tube for further analysis.


Note: RIPA lysis buffer can be added directly to the flask containing cells. Please see the following procedures.

1. Carefully remove culture medium from adherent cells.

2. Wash cells with chilled PBS. Carefully remove PBS.

3. Add chilled RIPA lysis buffer to the cells. Vortex briefly. Incubate on ice for 30 minutes. (For the volume of the lysis buffer, follow the instructions listed below)

SIZE of the plate/surface areaVolume of the lysis buffer
100mm500-1000μL
60mm250-500μL
6-well plate200-400μL per well
24-well plate100-200μL per well
96-well plate50-100μL per well

4. Centrifuge samples at 14000xg for 10 minutes.

5. Transfer supernatant to a new tube for further analysis.

Procedure for Lysis of Suspension-cultured Mammalian Cells

Note: Pre-chill an appropriate volume of RIPA Lysis Buffer at 4°C. If desired, add protease inhibitor and phosphatase inhibitor to the lysis buffer immediately before use.

1. In a microcentrifuge tube, harvest 5×106 cells by centrifugation at 600xg for 5min. Carefully remove and discard the supernatant.

2. Resuspend the cells in chilled PBS. Centrifuge at 600xg for 5min, then carefully remove and discard the supernatant.

3. Add 0.5 mL of chilled RIPA lysis buffer to the cell pellet. Vortex briefly. Incubate on ice for 30 minutes.

4. Centrifuge samples at 14000xg for 10 minutes.

5. Transfer supernatant to a new tube for further analysis.

Procedure for Lysis of Tissues

Note: Pre-chill an appropriate volume of RIPA Lysis Buffer at 4°C. If desired, add protease inhibitor and phosphatase inhibitor to the lysis buffer immediately before use.

1. Place the fresh tissue into chilled PBS and rinse several times. Mince the tissue into small pieces.

2. Add RIPA Lysis Buffer to the tissue at 10:1. (i.e., Add 10 mL chilled lysis buffer per gram of tissue.) Use a smaller volume of reagent if a more concentrated protein extract is required.

3. Homogenize for several minutes at high speed until no tissue chunks remain.

4. Incubate on ice for 30 minutes.

5. Centrifuge at ~10000 x g for 10 minutes.

6. Transfer supernatant to a new tube for further analysis.

Precautions

• All steps of protein lysis should be operated on ice or at 4°C.

• Use BCA Protein Assay kit (Product No. AR0146) to quantify lysed proteins. Bradford Protein Assay kit is not recommended.

• There might be some transparent gel complex containing genomic DNA in lysed proteins. The protein fractions can be used for further analysis after centrifugation if target proteins have little connection with genomic DNA. When detecting target proteins related closely to genomic DNA, sonicate gel complex and then centrifuge to collect supernatant for further analysis. Common transcription factors such as NFKB, p53 can be detected without sonication.

Product Images

AR0105-100 has been cited in 73 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Qu Y,Dou P,Hu M,Xu J,Xia W,Sun H.circRNA‑CER mediates malignant progression of breast cancer through targeting the miR‑136/MMP13 axis.Mol Med Rep.2019 Apr;19(4):3314-3320.doi:10.3892/mmr.2019.9965.Epub 2019 Feb 18.PMID:30816475.
Species: Human
AR0105-100 usage in article: APP:WB, SAMPLE: MCF-7 CELL AND ZR-75-30 CELL, DILUTION:NA

Lv J,Liu C,Chen FK,Feng ZP,Jia L,Liu PJ,Yang ZX,Hou F,Deng ZY.M2‑like tumour‑associated macrophage‑secreted IGF promotes thyroid cancer stemness and metastasis by activating the PI3K/AKT/mTOR pathway.Mol Med Rep.2021 Aug;24(2):604.doi:10.3892/mmr.2021.12249.Epub 2021 Jun 29. PMID:34184083.
Species: Human
AR0105-100 usage in article: APP:WB, SAMPLE:THP-1 CELL AND C643 CELL, DILUTION:NA

Chong Liu,Minmin Zhang,Shenyi Ye,Chenliang Hong,Jiaxi Chen,Ruyue Lu,Bingjie Hu,Weijun Yang,Bo Shen,Zhengyi Gu,"Acacetin Protects Myocardial Cells against Hypoxia-Reoxygenation Injury through Activation of Autophagy",Journal of Immunology Research,vol.2021,Article ID 9979843,12 pages,2021.https://doi.org/10.1155/2021/9979843
Species: Rat
AR0105-100 usage in article: APP:WB, SAMPLE:H9C2 CELL, DILUTION:NA

Chao Hu,Xiaobin Zhu,Taogen Zhang,Zhouming Deng,Yuanlong Xie,Feifei Yan,Lin Cai," Tanshinone IIA Inhibits Osteosarcoma Growth through a Src Kinase-Dependent Mechanism",Evidence-Based Complementary and Alternative Medicine,vol.2021,Article ID 5563691,15 pages,2021.https://doi. org/10.1155/2021/5563691
Species: Human,Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:U2-OS CELL AND MG-63 CELL, DILUTION:NA

Xu J,Wang J,He Z,Chen P,Jiang X,Chen Y,Liu X,Jiang J.LncRNA CERS6-AS1 promotes proliferation and metastasis through the upregulation of YWHAG and activation of ERK signaling in pancreatic cancer.Cell Death Dis.2021 Jun 24;12(7):648.doi:10.1038/s41419-021-03921-3.PMID:34168120.
Species: Human
AR0105-100 usage in article: APP:WB, SAMPLE:PC TISSUE,ASPC-1 CELL, BXPC-3 CELL AND HPDE CELL, DILUTION:NA

Yang LG,Wang AL,Li L,Yang H,Jie X,Zhu ZF,Zhang XJ,Zhao HP,Chi RF,Li B,Qin FZ,Wang JP,Wang K.Sphingosine-1-phosphate induces myocyte autophagy after myocardial infarction through mTOR inhibition.Eur J Pharmacol.2021 Jun 15:174260.doi:10.1016/j.ejphar.2021.174260.Epub ahead of print.PMID:34144026.
Species: Rat
AR0105-100 usage in article: APP:WB, SAMPLE:LV TISSUE, DILUTION:NA

Huang X,Pei W,Ni B,Zhang R,You H.Chondroprotective and antiarthritic effects of Galangin in Osteoarthritis: An in Vitro and in Vivo Study.Eur J Pharmacol.2021 Jun 3:174232.doi:10.1016/j. ejphar.2021. 174232.Epub ahead of print.PMID:34090897.
Species: Rat
AR0105-100 usage in article: APP:WB, SAMPLE:CHONDROCYTES, DILUTION:NA

Zhou X,Xu W,Wang Y,Zhang H,Zhang L,Li C,Yao S,Huang Z,Huang L,Luo D.LncRNA DNM3OS regulates GREM2 via miR-127-5p to suppress early chondrogenic differentiation of rat mesenchymal stem cells under hypoxic conditions.Cell Mol Biol Lett.2021 May 28;26(1):22.doi:10.1186/s11658-021-00269-6. PMID:34049478.
Species: Rat
AR0105-100 usage in article: APP:WB, SAMPLE:RMSCS, DILUTION:NA

Chang Z-p,Deng G-f,Shao Y-y,Xu D,Zhao Y-n,Sun Y-f,Zhang S-q,Hou R-g and Liu J-j(2021)Shaoyao-Gancao Decoction Ameliorates the Inflammation State in Polycystic Ovary Syndrome Rats via Remodeling Gut Microbiota and Suppressing the TLR4/NF-κB Pathway.Front.Pharmacol.12:670054.doi:10.3389/fphar.2021.670054
Species: Mouse,Rat
AR0105-100 usage in article: APP:WB, SAMPLE:RAW264.7 CELL, DILUTION:NA

Yating Li,Jiongjie Jing,Wenqing Dang,Qi Han,Xiangyu Guo,Kaiqi Jia,Ying Cheng,Kai Wang,Ermias Kebreab,Lihua Lyu,Effects of Notch2 on proliferation, apoptosis and steroidogenesis in bovine luteinized granulosa cells,Theriogenology,Volume 171,2021,Pages 55-63,ISSN 0093-691X,https://doi.org/10.1016/j.theriogenology.2021.05.009.
Species: Holstein cow
AR0105-100 usage in article: APP:WB, SAMPLE:LGCS, DILUTION:NA

Zhou,H.,Shi,P.,Jia,X.,& Xue,Q.(2021).Long non‑coding RNA LINC01018 inhibits the progression of acute myeloid leukemia by targeting miR‑499a‑5p to regulate PDCD4.Oncology Letters,22,541.https://doi.org/10.3892/ol.2021.12802
Species: Human
AR0105-100 usage in article: APP:WB, SAMPLE:HL-60 CELL AND THP-1 CELL, DILUTION:NA

Wang,L.,Gao,J.,Zhang,Y.,& Kang,S.(2021).Silencing miRNA‑1297 suppresses the invasion and migration of prostate cancer cells via targeting modulation of PTEN and blocking of the AKT/ERK pathway.Experimental and Therapeutic Medicine,22,768.https://doi.org/10.3892/etm.2021.10200
Species: Human
AR0105-100 usage in article: APP:WB, SAMPLE:PC‑3 CELL AND LNCAP CELL, DILUTION:NA

Xiao X,Bu H,Li Z,Li Z,Bai Q,Wang Z,Yan L,Liu D,Peng X,Jia X,Gao F.NADPH-Oxidase 2 Promotes Autophagy in Spinal Neurons During the Development of Morphine Tolerance.Neurochem Res.2021 May 18.doi:10.1007/s11064-021-03347-5.Epub ahead of print.PMID:34008119.
Species: Rat
AR0105-100 usage in article: APP:WB, SAMPLE:SPINAL CORD TISSUE, DILUTION:NA

Sun K,Zhang J,Yang Q,Zhu J,Zhang X,Wu K,Li Z,Xie W,Luo X.Dexmedetomidine exerts a protective effect on ischemic brain injury by inhibiting the P2X7R/NLRP3/Caspase-1 signaling pathway.Brain Res Bull.2021 May 12:S0361-9230(21)00137-4.doi:10.1016/j.brainresbull.2021.05.006.Epub ahead of print.PMID:33991606.
Species: Rat
AR0105-100 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:NA

Yan F,Hao Y,Gong X,Sun H,Ding J,Wang J.Silencing a disintegrin and metalloproteinase‑33 attenuates the proliferation of vascular smooth muscle cells via PI3K/AKT pathway: Implications in the pathogenesis of airway vascular remodeling.Mol Med Rep.2021 Jul;24(1):502.doi:10.3892/mmr.2021.12141.Epub 2021 May 13.PMID:33982767.
Species: Human
AR0105-100 usage in article: APP:WB, SAMPLE:HASMCS, DILUTION:NA

Jun-Liang Pu,Zuo-Tian Huang,Yun-Hai Luo,Tong Mou,Ting-Ting Li,Zhong-Tang Li,Xu-Fu Wei,Zhong-Jun Wu,Fisetin mitigates hepatic ischemia-reperfusion injury by regulating GSK3β/AMPK/NLRP3 inflammasome pathway,Hepatobiliary & Pancreatic Diseases International,2021,,ISSN 1499-3872,https://doi.org/10.1016/j.hbpd.2021.04.013.
Species: Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:RAW264.7 CELL AND HEPATIC TISSUE, DILUTION:NA

Wang J,Yang L,Li Y,Wang XB,Yang W,Liu F,Wu XB.Aberrant hypermethylation induced downregulation of antisense lncRNA STXBP5-AS1 and its sense gene STXBP5 correlate with tumorigenesis of glioma.Life Sci.2021 May 6;278:119590.doi:10.1016/j.lfs.2021.119590.Epub ahead of print.PMID:33965377.
Species: Human,Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:GLIOMA CELL, DILUTION:NA

Jing X,Du T,Li T,Yang X,Wang G,Liu X,Jiang Z,Cui X.The detrimental effect of iron on OA chondrocytes: Importance of pro-inflammatory cytokines induced iron influx and oxidative stress.J Cell Mol Med.2021 May 3.doi:10.1111/jcmm.16581.Epub ahead of print.PMID:33942503.
Species: Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:CHONDROCYTES, DILUTION:NA

Yang Y,Yi J,Pan M,Hu B,Duan H.Edaravone alleviated propofol-induced neural injury in developing rats by BDNF/TrkB pathway.J Cell Mol Med.2021 May 1.doi:10.1111/jcmm.16422.Epub ahead of print.PMID:33932098.
Species: Rat
AR0105-100 usage in article: APP:WB, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:NA

Xuyang Lv,Jiangchuan Sun,Linfeng Hu et al.Curcumol inhibits malignant biological behaviors and TMZ- resistance of glioma cells via reducing long noncoding RNA FoxD2-As1 promoted EZH2 activation, 13 April 2021,PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-415245/v1]
Species: Human,Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:GLIOMA CELL, DILUTION:NA

Shan,W.,Zhou,L.,Liu,L.,Lin,D.,& Yu,Q.(2021).Polycomb group protein Bmi1 is required for the neuronal differentiation of mouse induced pluripotent stem cells. Experimental and Therapeutic Medicine,21,619.https://doi.org/10.3892/etm.2021.10051
Species: Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:IPSC DERIVED CELLS, DILUTION:NA

Liu Q, Zhou J, Yang Z, Xie C, Huang Y, Ling L, Cao Y, Hu H, Hua Y. The Ginsenoside Exhibits Antiosteoporosis Effects in Ketogenic-Diet-Induced Osteoporosis via Rebalancing Bone Turnover. Front Pharmacol. 2021 Jan 14;11:593820. doi: 10.3389/fphar.2020.593820. PMID: 33519454; PMCID: PMC7840492.
Species: Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:TIBIA TISSUE, DILUTION:NA

Yin J, Tu G, Peng M, Zeng H, Wan X, Qiao Y, Qin Y, Liu M, Luo H. GPER-regulated lncRNA-Glu promotes glutamate secretion to enhance cellular invasion and metastasis in triple-negative breast cancer. FASEB J. 2020 Mar;34(3):4557-4572. doi: 10.1096/fj.201901384RR. Epub 2020 Feb 6. PMID: 32030797.
Species: Human,Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:MDA-MB-468 CELL AND HCC1806 CELL, DILUTION:NA

Chen T,Lei S,Zeng Z,Zhang J,Xue Y,Sun Y,Lan J,Xu S,Mao D,Guo B.Linc00261 inhibits metastasis and the WNT signaling pathway of pancreatic cancer by regulating a miR‑552‑5p/FOXO3 axis.Oncol Rep.2020 Mar;43(3):930-942.doi:10.3892/or.2020.7480.Epub 2020 Jan 27.PMID:32020223;PMCID:PMC7041108.
Species: Human,Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:PC CELLS, DILUTION:NA

Cheng B,Li X,Dai K,Duan S,Rong Z,Chen Y,Lü L,Liu Z,Huang X,Xu H,Zhang YW,Zheng H.Triggering Receptor Expressed on Myeloid Cells-2 (TREM2) Interacts With Colony-Stimulating Factor 1 Receptor (CSF1R) but Is Not Necessary for CSF1/CSF1R-Mediated Microglial Survival.Front Immunol.2021 Mar 25;12:633796.doi:10.3389/fimmu.2021.633796.PMID:33841415;PMCID:PMC8027073.
Species: Human,Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:MICROGLIAL CELL, DILUTION:NA

Huang,H.,Liu,K.,Ou,H.,Qian,X.,& Wan,J.(2021).Phgdh serves a protective role in Il‑1β induced chondrocyte inflammation and oxidative‑stress damage.Molecular Medicine Reports,23,419.https://doi.org/10.3892/mmr.2021.12058
Species: Rat
AR0105-100 usage in article: APP:WB, SAMPLE:CHONDROCYTES, DILUTION:NA

Xu Q,Sun L, Zhang W,Wu H,Jiao C,Xu L,Qian X,Yao H,Chen Q,Xu F,Fu F,Feng Y,Wang L,Chen X.A novel visceral pain model of uterine cervix inflammation in rat.Eur J Pharmacol.2021 Mar 31;900:174080.doi:10.1016/j.ejpha r.2021.174080.Epub ahead of print.PMID:33811839.
Species: Rat
AR0105-100 usage in article: APP:WB, SAMPLE:UTERINE CERVIX TISSUE, DILUTION:NA

Ni J,Jiang L,Shen G,Xia Z,Zhang L,Xu J,Feng Q,Qu H,Xu F,Li X.Hydrogen sulfide reduces pyroptosis and alleviates ischemia-reperfusion acute kidney injury by inhibiting NLRP3 inflammasome.Life Sci.2021 Mar 31:119466.doi:10.1016/j.lfs.2021.119466.Epub ahead of print.PMID:33811893.
Species: Human,Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE AND HK-2 CELL, DILUTION:NA

Zhang W,Wang Z,Cai G,Huang P.Downregulation of Circ_0071589 Suppresses Cisplatin Resistance in Colorectal Cancer by Regulating the MiR-526b-3p/KLF12 Axis.Cancer Manag Res.2021 Mar 23;13:2717-2731.doi:10.2147/CMAR.S294880.PMID:33790646;PMCID:PMC8001125.
Species: Human,Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:CRC CELL LINE AND FHC CELL, DILUTION:NA

Zhou H, Fang Q, Li N, Yu M, Chen H, Guo S. ASMq protects against early burn wound progression in rats by alleviating oxidative stress and secondary mitochondria‑associated apoptosis via the Erk/p90RSK/Bad pathway. Mol Med Rep. 2021 May;23(5):390. doi: 10.3892/mmr.2021.12029. Epub 2021 Mar 24. PMID: 33760179.
Species: Rat
AR0105-100 usage in article: APP:WB, SAMPLE:SKIN TISSUE, DILUTION:NA

Gao Y,Xu Y,Zhao S,Qian L,Song T,Zheng J,Zhang J,Chen B. Growth differentiation factor-15 promotes immune escape of ovarian cancer via targeting CD44 in dendritic cells. Exp Cell Res.2021 Mar 23:112522.doi:10.1016/j.yexcr.2021.112522.Epub ahead of print.PMID:33771482.https://pubmed.ncbi.nlm.nih.gov/33771 4 82/
Species: BA1105;APP:IF;SAMPLE:OVARIAN TISSUE;DILUTION:NA| BA1001;APP:IF;SAMPLE:OVARIAN TISSUE;DILUTION:NA|AR1176 AR1177;APP:IF;SAMPLE:OVARIAN TISSUE;DILUTION:NA|M00602-2;APP:CONFOCAL MICROSCOPY;SAMPLE:DCS;DILUTION:1:20|BA1142;APP: CONFOCAL MICROSCOPY;SAMPLE:DCS;DILUTION:NA|BA1001;APP:CONFOCAL MICROSCOPY;SAMPLE:DCS;DILUTION:NA|AR0107;APP:CO-IP;SAMPLE:DCS;DILUTION:NA|AR0105-100;APP:WB;SAMPLE:DCS; DILUTION:NA|BA1054;APP:WB;SAMPLE:DCS;DILUTION:NA

Hu Y, Huang W, Luo Y, Xiang L, Wu J, Zhang Y, Zeng Y, Xu C, Meng X, Wang P. Assessment of the Anti-Inflammatory Effects of Three Rhubarb Anthraquinones in LPS-Stimulated RAW264.7 Macrophages Using a Pharmacodynamic Model and Evaluation of the Structure-Activity Relationships. J Ethnopharmacol. 2021 Mar 16: 114027.doi:10.1016/j.jep.2021.114027. Epub ahead of print. PMID: 33741438.
Species: Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:RAW264.7 CELL, DILUTION:NA

Penghui Zhang,Xizhe Liu,Peng Guo,Xianlong Li,Zhongyuan He,Zhen Li,Martin J. Stoddart,Sibylle Grad,Wei Tian, Dafu Chen,Xuenong Zou,Zhiyu Zhou,Shaoyu Liu, Effect of cyclic mechanical loading on immunoinflammatory microenvironment in biofabricating hydroxyapatite scaffold for bone regeneration, Bioactive Materials,Volume 6,Issue 10,2021,Pages 3097-3108,ISSN 2452-199X,https: //doi.org/10.1016/j.bioactmat.2021.02.024.
Species: Human,New Zealand White Rabbit,Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:RAW264.7 CELL, DILUTION:NA

Gao J,Teng L,Yang S,Huang S,Li L,Zhou L,Liu G,Tang H. MNK as a potential pharmacological target for suppressing LPS-induced acute lung injury in mice. Biochem Pharmacol.2021 Mar 4;186:114499.doi:10.1016/j.bcp.2021.114499.Epub ahead of print.PMID:33675774;PMCID:PMC7957947.
Species: Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:BMDMS, DILUTION:NA

Guo J,Ren R,Yao X,Ye Y,Sun K,Lin J,Wang G, Guo F,Xiao J,Xu T.PKM2 suppresses osteogenesis and facilitates adipogenesis by regulating β-catenin signaling and mitochondrial fusion and fission.Aging (Albany NY).2020 Feb 25;12(4):3976-3992.doi:10. 18632/aging
Species: Mouse
AR0105-100 usage in article: APP:MEASUREMENT OF ALP ACTIVITY, SAMPLE:BMSCS, DILUTION:NA

Yiqiang Sun, Xia Wang, Guoyong Fu, Xiaopeng Geng,microRNA-199a-5p accelerates nucleus pulposus cell apoptosis and intervertebral disc degeneration by inhibiting SIRT1-mediated deacetylation of p21,Molecular Therapy - Nucleic Acids,2021,ISSN 2162-2531,http
Species: Human,Rat
AR0105-100 usage in article: APP:WB, SAMPLE:PULPOSUS CELL, DILUTION:NA

Guochun Ou,Qin Liu,Chengxiu Yu,Xiaoju Chen,Wenbo Zhang,Yong Chen,Tao Wang,Yongqiang Luo,Guolu Jiang,Mingmei Zhu,Hongmei Li,Mei Zeng,"The Protective Effects of Maresin 1 in the OVA-Induced Asthma Mouse Model",Mediators of Inflammation,vol.2021,Article ID 4
Species: Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:LUNG TISSUE, DILUTION:NA

Zhang R,Ji J,Zhou X,Li R.Irisin Pretreatment Protects Kidneys against Acute Kidney Injury Induced by Ischemia/Reperfusion via Upregulating the Expression of Uncoupling Protein 2. Biomed Res Int.2020 Aug 31;2020:6537371.doi:10.1155/2020/6537371.PMID:329349
Species: Human,Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:HK-2 CELL AND KIDNEY TISSUE, DILUTION:NA

Zhang XY,Guo Z,Li TP,Sun T.Dietary capsaicin normalizes CGRP peptidergic DRG neurons in experimental diabetic peripheral neuropathy.Sci Rep.2021 Jan 18;11(1):1704.doi:10.1038/s41598-021-81427-w.PMID:33462325;PMCID:PMC7814129.
Species: Rat
AR0105-100 usage in article: APP:ELISA, SAMPLE:SPINAL TISSUE, DILUTION:NA

Deng X,He Y,Miao X,Yu B.ATF4-mediated histone deacetylase HDAC1 promotes the progression of acute pancreatitis.Cell Death Dis.2021 Jan 4;12(1):5.doi:10. 1038/s41419-020-03296-x.PMID:33414424.
Species: Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:PANCREATIC TISSUE, DILUTION:NA

Zhang F,Liu X,Li B,Li Z,Grad S,Chen D,Gao M,Liu S.The effect of hyaluronic acid on nucleus pulposus extracellular matrix production through hypoxia-inducible factor-1α transcriptional activation of CD44 under hypoxia.Eur Cell Mater.2021 Feb 3;41:142-152.d
Species: Human
AR0105-100 usage in article: APP:WB, SAMPLE:NPCS, DILUTION:NA

Lan Y,Dong M,Li Y,Diao Y,Chen Z,Li Y,SIRT1-Induced Deacetylation of Akt Expedites Platelet Phagocytosis and Delays Human Endometrial Microvascular Endothelial Cell Aging,Molecular Therapy:Nucleic Acid (2021),doi:https://doi.org/10.1016/j.omtn.2021.01.023.
Species: Human
AR0105-100 usage in article: APP:WB:SAMPLE:PLATELETS, DILUTION:NA

Guo J,Guo M,Zheng J. Inhibition of Bone Morphogenetic Protein 2 Suppresses the Stemness Maintenance of Cancer Stem Cells in Hepatocellular Carcinoma via the MAPK/ERK Pathway. Cancer Manag Res.2021 Jan 27;13:773-785.doi:10.2147/CMAR.S281969.PMID:33536785;P
Species: Human
AR0105-100 usage in article: APP:WB, SAMPLE:HCC TISSUE AND HCC CELLS, DILUTION:NA

Guo S,Fang Q,Chen L,Yu M,Chen Y,Li N,Han C,Hu X.Locally activated mitophagy contributes to a "built-in" protection against early burn-wound progression in rats.Life Sci.2021 Jan 22:119095.doi:10.1016/j.lfs.2021.119095.Epub ahead of print.PMID:33493522.
Species: Rat
AR0105-100 usage in article: APP:WB, SAMPLE:SKIN TISSUE, DILUTION:NA

Weizhang Xu,Yun Hu,Haifei Xu et al.The Oncogenic Capacities of Long Noncoding RNA SNHG1 in Bladder Cancer by inducing proliferation and repressing apoptosis via upregulation of microRNA-9-3p-targeted MDM2,08 January 2021,PREPRINT (Version 1) available at
Species: Human,Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:BLADDER CELL, DILUTION:NA

Yi L,Ju Y,He Y,Yin X,Xu Y,Weng T.Intraperitoneal injection of Desferal® alleviated the age-related bone loss and senescence of bone marrow stromal cells in rats.Stem Cell Res Ther.2021 Jan 7;12(1):45.doi:10.1186/s13287-020-02112-9.PMID:33413663;PMCID:PMC7
Species: Rat
AR0105-100 usage in article: APP:WB, SAMPLE:BMSCS, DILUTION:NA

Duozhi Chen,Shirui Fan,Xueying Lu,et al.Axin, the classic scaffold protein of Wnt/β-catenin signalling as a potential drug-target for vitiligo.Authorea.November 19, 2020.DOI:10.22541/au.160581793.37973769/v1
Species: Mouse
AR0105-100 usage in article: APP:PROTEIN QUANTIFICATION, SAMPLE:B16 CELLS, DILUTION:NA

Yao X,Sun K,Yu S,Luo J,Guo J,Lin J,Wang G,Guo Z,Ye Y,Guo F. Chondrocyte ferroptosis contribute to the progression of osteoarthritis. J Orthop Translat. 2020 Dec 17;27:33-43. doi:10.1016/j.jot. 2020.09.006.PMID:33376672;PMCID:PMC7750492.
Species: Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:CHONDROCYTE, DILUTION:NA

Wenchang Lv,Yuping Ren,Min Wu,Xiao Luo,Jing Yu,Qi Zhang,Yiping Wu, Identifying miRNA modules associated with progression of keloids through weighted gene co-expression network analysis and experimental validation in vitro, Burns, 2020, ISSN 0305-4179,http
Species: Human
AR0105-100 usage in article: APP:WB, SAMPLE:KELOID TISSUE AND SKIN TISSUE, DILUTION:NA

Qin,Y.,Hou,Y.,Liu,S.,Zhu,P.,Wan,X.,Zhao,M.,Peng,M.,Zeng,H.,Li,Q.,Jin,T.,Cui,X.,Liu, M.,A Novel Long Non‐Coding RNA lnc030 Maintains Breast Cancer Stem Cell Stemness by Stabilizing SQLE mRNA and Increasing Cholesterol Synthesis. Adv. Sci.2020, 2002232. htt
Species: Human
AR0105-100 usage in article: APP:WB, SAMPLE:BT549 CELLS,HS578T CELLS AND MCF7 CELLS, DILUTION:NA

Daixing Hu,Li Jiang,Shengjun Luo et al. c-Jun Transcriptional Regulates NPRL2 to Promote the Proliferation Activity of Prostate Cancer Cells via AKT/MDM2/p53 Signaling Pathway, 19 November 2020,PREPRINT (Version 1) available at Research Square[+https://do
Species: Human,Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:PC3 CELLS AND LNCAP CELLS, DILUTION:NA

Wang Q,Suo Y,Wang X,Wang Y,Tian X,Gao Y,Liu N,Liu R.Study on the mechanism of photodynamic therapy mediated by 5-aminoketovalerate in human ovarian cancer cell line.Lasers Med Sci.2021 Jan 3.doi:10.1007/s10103-020-03226-5.Epub ahead of print.PMID:33392781
Species: Human
AR0105-100 usage in article: APP:WB, SAMPLE:OVCAR3 CELL, DILUTION:NA

Li M,Xie X,Chen H,Xiong Q,Tong R,Peng C,Peng F.Aconitine induces cardiotoxicity through regulation of calcium signaling pathway in zebrafish embryos and in H9c2 cells.J Appl Toxicol.2020 Jun;40(6):780-793.doi:10.1002/jat.3943.Epub 2020 Jan 23.PMID:3197543
Species: Zebrafish,Rat
AR0105-100 usage in article: APP:WB, SAMPLE:H9C2 CELL, DILUTION:NA

Huang L,Zhao C,Sun K,Yang D,Yan L,Luo D,He J,Hu X,Wang R,Shen X,Xiao N,Zhong Z.Downregulation of CLDN6 inhibits cell proliferation, migration, and invasion via regulating EGFR/AKT/mTOR signalling pathway in hepatocellular carcinoma.Cell Biochem Funct.2020
Species: Human
AR0105-100 usage in article: APP:WB, SAMPLE:HEPG2 CELL, DILUTION:NA

Xu L,Shen J,Dai S,Sun L,Chen X. Tetramethylpyrazine Attenuated Sevoflurane-Induced Neurotoxicity by Enhancing Autophagy through GPR50/CREB Pathway in SH-SY5Y Cells. Am J Chin Med.2020;48(4):945-966.doi: 10.1142/S0192415X20500457.Epub 2020 May 30.PMID:3247
Species: Human
AR0105-100 usage in article: APP:WB, SAMPLE:SH-SY5Y CELL, DILUTION:NA

Zhao H,Li R,Wang X,Lu X,Hu M,Zhang J,Zhao X,Song X,Liu Y. The role of apatinib combined with paclitaxel (aluminum binding type) in platinum-resistant ovarian cancer. J Ovarian Res.2020 Sep 21;13(1):113. doi:10.1186/s13048-020-00719-3.PMID: 32958014;PMCID:
Species: Human,Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:SKOV-3/DDP CELL, DILUTION:NA

Ni B,Huang X,Xi Y,Mao Z,Chu X,Zhang R,Ma X,You H.Neferine Inhibits Expression of Inflammatory Mediators and Matrix Degrading Enzymes in IL-1β-Treated Rat Chondrocytes via Suppressing MAPK and NF-κB Signaling Pathways.Inflammation.2020 Aug;43(4):1209-1221.
Species: Rat
AR0105-100 usage in article: APP:WB, SAMPLE:CHONDROCYTES, DILUTION:NA

Su Q,Li T,He PF,Lu XC,Yu Q,Gao QC,Wang ZJ,Wu MN,Yang D,Qi JS.Trichostatin A ameliorates Alzheimer‘s disease-related pathology and cognitive deficits by increasing albumin expression and Aβ clearance in APP/PS1 mice.Alzheimers Res Ther.2021 Jan 4;13(
Species: Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:NA

Wan SY,Li GS,Tu C,Chen WL,Wang XW,Wang YN,Peng LB,Tan F.MicroNAR-194-5p hinders the activation of NLRP3 inflammasomes and alleviates neuroinflammation during intracerebral hemorrhage by blocking the interaction between TRAF6 and NLRP3.Brain Res.2020 Dec 2
Species: Rat
AR0105-100 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:NA

Liu H,Xiong D,Pang R,Deng Q,Sun N,Zheng J,Liu J,Xiang W,Chen Z,Lu J,Wang W,Zhang A.Effects of repetitive magnetic stimulation on motor function and GAP43 and 5-HT expression in rats with spinal cord injury.J Int Med Res.2020 Dec;48(12):300060520970765.doi
Species: Rat
AR0105-100 usage in article: APP:WB, SAMPLE:SPINAL CORD TISSUE, DILUTION:NA

Ge M,Yin J,Qin X,Zhai J,Shao X,Wang R,Fan S,Dai Z,Wang S,Li Y.Isoflurane post-conditioning attenuates cerebral ischemia/reperfusion injury by reducing apoptotic through activating the BMP7/SMAD signaling pathway in rats.J Chem Neuroanat.2020 Dec 26;112:10
Species: Rat
AR0105-100 usage in article: APP:WB, SAMPLE:CEREBRAL TISSUE, DILUTION:NA

Hu L,Lv X,Li D,Zhang W,Ran G,Li Q,Hu J. The anti-angiogenesis role of FBXW7 in diabetic retinopathy by facilitating the ubiquitination degradation of c-Myc to orchestrate the HDAC2.J Cell Mol Med. 2020 Dec 25.doi: 10.1111/jcmm.16204.Epub ahead of print.PM
Species: Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:RETINAL TISSUE, DILUTION:NA

Yao X,Yu S,Jing X,Guo J,Sun K,Guo F,Ye Y.PTEN inhibitor VO-OHpic attenuates GC-associated endothelial progenitor cell dysfunction and osteonecrosis of the femoral head via activating Nrf2 signaling and inhibiting mitochondrial apoptosis pathway.Stem Cell
Species: Rat
AR0105-100 usage in article: APP:WB, SAMPLE:EPCS, DILUTION:NA

Yang S,Wang H,Qin C,Sun H,Han Y.Up-regulation of CXCL8 expression is associated with a poor prognosis and enhances tumor cell malignant behaviors in liver cancer. Biosci Rep.2020 Aug 28;40(8):BSR20201169.doi: 10.1042/BSR20201169.PMID:32766720;PMCID:PMC744
Species: Human
AR0105-100 usage in article: APP:WB, SAMPLE:HEPG 2 CELL, DILUTION:NA

Jing X,Du T,Yang X,Zhang W,Wang G,Liu X,Li T,Jiang Z.Desferoxamine protects against glucocorticoid-induced osteonecrosis of the femoral head via activating HIF-1α expression.J Cell Physiol.2020 Dec;235(12):9864-9875.doi:10.1002/jcp.29799.Epub 2020 May 21.
Species: Rat
AR0105-100 usage in article: APP:WB, SAMPLE:RAT BMSCS, DILUTION:NA

Cai P,Lu Z,Jiang T,Wang Z,Yang Y,Zheng L,Zhao J.Syndecan-4 involves in the pathogenesis of rheumatoid arthritis by regulating the inflammatory response and apoptosis of fibroblast-like synoviocytes.J Cell Phys iol.2020 Feb;235(2):1746-1758.doi:10.1002/jcp
Species: Human
AR0105-100 usage in article: APP:WB, SAMPLE:FLSS, DILUTION:NA

Sun K,Luo J,Jing X,Xiang W,Guo J,Yao X,Liang S,Guo F,Xu T.Hyperoside ameliorates the progression of osteoarthritis: An in vitro and in vivo study.Phytomedicine.2020 Oct 14;80:153387.doi:10.1016/j.phymed.2020. 153387.Epub ahead of print.PMID:33130473.
Species: Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:CHONDROCYTES, DILUTION:NA

Ma Y,Yang L,Li R.HnRNPA2/B1 Is a Novel Prognostic Biomarker for Breast Cancer Patients.Genet Test Mol Biomarkers.2020 Nov;24(11):701-707.doi:10.1089/gtmb.2020.0086.Epub 2020 Sep 25.PMID:32985904.
Species: Human
AR0105-100 usage in article: APP:WB, SAMPLE:BREAST TISSUE , DILUTION:NA

Wang Y,Liu L,Tao H,Wen L,Qin S.TRPC6 participates in the development of blood pressure variability increase in sino-aortic denervated rats. Heart Vessels. 2020 Dec;35(12):1755-1765.doi: 10.1007/s00380-020-01682-1.Epub 2020 Aug 25.PMID: 32844288.
Species: Rat
AR0105-100 usage in article: APP:WB, SAMPLE:MYOCARDIAL AND THORACIC AORTIC TISSUE, DILUTION:NA

Yang S,Chen R,Zhang L,Sun Q,Li R,Gu W,Zhong M,Liu Y,Chen LC,Sun Q,Liu C.Lipid metabolic adaption to long-term ambient PM2.5 exposure in mice. Environ Pollut. 2020 Nov 30;269:116193.doi: 10.1016/j.envpol.2020.116193.Epub ahead of print.PMID: 33302088.
Species: Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA

Zhang L, Sun D, Zhang J, Tian Y. Circ-UBR1 facilitates proliferation, metastasis, and inhibits apoptosis in breast cancer by regulating the miR-1299/CCND1 axis. Life Sci. 2020 Nov 28;266:118829. doi: 10.1016/j.lfs.2020.118829. Epub ahead of print. PMID: 3
Species: Human, Mouse
AR0105-100 usage in article: APP:WB, SAMPLE:T47D CELLS AND MCF-7 CELLS, DILUTION:NA

Xiao N, Li C, Liao W, Yin J, Zhang S, Zhang P, Yuan L, Hong M. FOXG1 mediates the radiosensitivity of glioma cells through regulation of autophagy. Int J Radiat Biol. 2020 Nov 17:1-32. doi:10.1080/09553002.2021.18 46816. Epub ahead of print. PMID: 3320174
Species: Human
AR0105-100 usage in article: APP:WB, SAMPLE:U87 MG CELLS, DILUTION:NA

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2 Customer Q&As for RIPA Lysis Buffer

Question

What buffer is recommended to be used to isolate protein from tumor tissue?

Verified customer

Asked: 2021-01-26

Answer

RIPA Lysis Buffer (AR0105-100) is recommended to be used to isolate protein from tumor tissue.

Boster Scientific Support

Answered: 2021-01-26

Question

What is the recommended method of sample preparation in regards to the Human TNFa kit for isolating brain tumor tissue and serum? Would AR0105-100 suffice?

Verified customer

Asked: 2019-04-30

Answer

The Ripa lysis buffer would suffice for preparing the brain tissue. RIPA Lysis Buffer (AR0105-100) Please see our sample preparation guide: https://www.bosterbio.com/protocol-and-troubleshooting/elisa-sample-preparation-guide

Boster Scientific Support

Answered: 2019-04-30

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