|Applications|| Western blotting, ELISA, immunofluorescence, Immunoprecipitation; protein purification; SDS-PAGE; protein assays; epitope tagging; reporter gene analysis|
|Product Name||RIPA Lysis Buffer|
|Synonyms||RIPA buffer; Radio-immunoprecipitation assay buffer, RIPA Lysis and Extraction Buffer|
|Description||RIPA Lysis Buffer is a complete cell lysis solution reagent used for rapid and efficient total cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells, effectively extracting cytoplasmic, nuclear and membrane proteins. RIPA lysis buffer is highly compatible with various downstream protein analysis applications.|
|Application||Western blotting, ELISA, immunofluorescence, Immunoprecipitation; protein purification; SDS-PAGE; assays for protein expression, activity, modification, profiling, characterization and quantitative measurement; epitope tagging; reporter gene analysis|
|Pack Size||50 ml||Reagent Type||Western Blotting related reagent; Cell lysis buffer; Universal tissue extraction buffer; Detergent solution||Content||1% NP-40, 0.5% Sodium deoxycholate, 0.1% SDS||Usage||Extraction of cytoplasmic, membrane and nuclear proteins|
|Form Supplied||Ready-to-use 1X solution|
|Pack Size||50 ml|
|Recommended working concentration||10 ml RIPA Lysis Buffer per gram of tissue|
|0.5 ml RIPA Lysis Buffer per 5.0x106 cells in suspension|
|0.5 ml RIPA Lysis Buffer per 5.0x106 adherent mammalian cells|
|0.5-1 ml RIPA lysis buffer per subconfluent monolayer on a 100 mm plate|
|Compatibility with reagents||Fully compatible with Broad Spectrum Protease Inhibitor Cocktail and Broad Spectrum Phosphatase Inhibitor Cocktail|
|Storage||Store at 4˚C for one year.|
|NP-40||Non-ionic detergent to extract proteins|
|Sodium deoxycholate||Ionic detergent to extract membrane proteins|
|SDS||Ionic detergents to extract proteins|
Usage and Handling
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. RIPA lysis buffer is highly compatible with immunoassays, protein purification procedures, immunoprecipitation, and western blotting.
RIPA lysis extraction buffer contains non-ionic and ionic detergents which are able to extract protein from wide variety of cell types and membrane structures. RIPA buffer ensures efficient cell lysis and protein solubilization preventing protein degradation and interference with protein immunoreactivity and biological activity. Since most antibodies and protein antigens are not adversely affected by the components of this solution, RIPA buffer-conducted protein extraction is compatible with various downstream immunoprecipitation and molecular pull-down assays. RIPA buffer reagent minimizes non-specific protein-binding interactions to keep background low, while allowing most specific interactions to occur, enabling studies of relevant protein-protein interactions.
RIPA Lysis Buffer Images
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50 mL per pack, Solution (liquid)