Show Order Info
SKU AR0105
Product name RIPA Lysis Buffer
Pack size 50ml
Form Solution (liquid)
Applications Western blotting, ELISA, immunofluorescence, Immunoprecipitation; protein purification; SDS-PAGE; protein assays; epitope tagging; reporter gene analysis

Overview

Form Supplied Ready-to-use 1X solution
Physical State Liquid
Pack Size 50 mL
Content 25mM Tris•HCl pH 7.6, 150mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS
Recommended working concentration 10 mL RIPA Lysis Buffer per gram of tissue
0.5 mL RIPA Lysis Buffer per 5.0x106 cells in suspension
0.5 mL RIPA Lysis Buffer per 5.0x106 adherent mammalian cells
Storage & Expiration Upon receipt store at 4°C. RIPA Lysis Buffer is stable for one year. Product is shipped on ice.
Assays per kit 100 assays for 5.0x106 cells
50 assays for 0.1g tissue
Compatibility with reagents Fully compatible with Broad Spectrum Protease Inhibitor Cocktail and Broad Spectrum Phosphatase Inhibitor Cocktail
Equivalent Thermofisher (Product No. 89900, 89901), Millipore Sigma (Product No. R0278)
Reagent Type Western Blotting related reagent; Cell lysis buffer; Universal tissue extraction buffer; Detergent solution
Usage Extraction of cytoplasmic, membrane and nuclear proteins
Cite This Product RIPA Lysis Buffer (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR0105)

Description

RIPA Lysis Buffer is a complete cell lysis solution reagent used for rapid and efficient total cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells, effectively extracting cytoplasmic, nuclear and membrane proteins. Protein lysis can be finished in 60 minutes.

Background

RIPA lysis extraction buffer contains non-ionic and ionic detergents which are able to extract protein from wide variety of cell types and membrane structures. RIPA buffer ensures efficient cell lysis and protein solubilization preventing protein degradation and interference with protein immunoreactivity and biological activity. Since most antibodies and protein antigens are not adversely affected by the components of this solution, RIPA buffer-conducted protein extraction is compatible with various downstream immunoprecipitation and molecular pull-down assays, including reporter assays, protein assays, immunoassays and protein purification. RIPA buffer reagent minimizes non-specific protein-binding interactions to keep background low, while allowing most specific interactions to occur, enabling studies of relevant protein-protein interactions.

Important Product Information

• If desired, add protease inhibitor (Product No. AR1182) and phosphatase inhibitor (Product No. AR1183) to the lysis buffer to prevent proteolysis and maintain phosphorylation status of proteins.

• Some protein kinases and other enzymes may be sensitive to the components of the RIPA Lysis Buffer, resulting in their decreased activity. In such cases, prepare a RIPA Lysis Buffer that does not contain sodium deoxycholate and SDS.

Additional Materials Required

• Protease inhibitor (Product No. AR1182) and phosphatase inhibitor (Product No. AR1183)

• 2 ml microcentrifuge tubes

• Tissue homogenizer

• Microcentrifuge capable of spinning at 10,000 x g

• Cell scraper

Procedure for Lysis of Monolayer-cultured Adherent Mammalian Cells

Note: Pre-chill an appropriate volume of RIPA Lysis Buffer at 4°C. If desired, add protease inhibitor and phosphatase inhibitor to the lysis buffer immediately before use.

1. In a microcentrifuge tube, resuspend 5×106 cells in the growth media by scraping the cells off the surface of the plate with a cell scraper. Centrifuge harvested cell suspension at 600xg for 5min, then carefully remove and discard the supernatant.

2. Resuspend the cells in chilled PBS. Centrifuge at 600xg for 5min, then carefully remove and discard the supernatant.

3. Add 0.5 mL of chilled RIPA lysis buffer to the cell pellet. Vortex briefly. Incubate on ice for 30 minutes.

4. Centrifuge samples at 14000xg for 10 minutes.

5. Transfer supernatant to a new tube for further analysis.


Note: RIPA lysis buffer can be added directly to the flask containing cells. Please see the following procedures.

1. Carefully remove culture medium from adherent cells.

2. Wash cells with chilled PBS. Carefully remove PBS.

3. Add chilled RIPA lysis buffer to the cells. Vortex briefly. Incubate on ice for 30 minutes. (For the volume of the lysis buffer, follow the instructions listed below)

SIZE of the plate/surface areaVolume of the lysis buffer
100mm500-1000μL
60mm250-500μL
6-well plate200-400μL per well
24-well plate100-200μL per well
96-well plate50-100μL per well

4. Centrifuge samples at 14000xg for 10 minutes.

5. Transfer supernatant to a new tube for further analysis.

Procedure for Lysis of Suspension-cultured Mammalian Cells

Note: Pre-chill an appropriate volume of RIPA Lysis Buffer at 4°C. If desired, add protease inhibitor and phosphatase inhibitor to the lysis buffer immediately before use.

1. In a microcentrifuge tube, harvest 5×106 cells by centrifugation at 600xg for 5min. Carefully remove and discard the supernatant.

2. Resuspend the cells in chilled PBS. Centrifuge at 600xg for 5min, then carefully remove and discard the supernatant.

3. Add 0.5 mL of chilled RIPA lysis buffer to the cell pellet. Vortex briefly. Incubate on ice for 30 minutes.

4. Centrifuge samples at 14000xg for 10 minutes.

5. Transfer supernatant to a new tube for further analysis.

Procedure for Lysis of Tissues

Note: Pre-chill an appropriate volume of RIPA Lysis Buffer at 4°C. If desired, add protease inhibitor and phosphatase inhibitor to the lysis buffer immediately before use.

1. Place the fresh tissue into chilled PBS and rinse several times. Mince the tissue into small pieces.

2. Add RIPA Lysis Buffer to the tissue at 10:1. (i.e., Add 10 mL cilled lysis buffer per gram of tissue.) Use a smaller volume of reagent if a more concentrated protein extract is required.

3. Homogenize for several minutes at high speed until no tissue chunks remain.

4. Incubate on ice for 30 minutes.

5. Centrifuge at ~10000 x g for 10 minutes.

6. Transfer supernatant to a new tube for further analysis.

Precautions

• All steps of protein lysis should be operated on ice or at 4°C.

• Use BCA Protein Assay kit (Product No. AR0146) to quantify lysed proteins. Bradford Protein Assay kit is not recommended.

• There might be some transparent gel complex containing genomic DNA in lysed proteins. The protein fractions can be used for further analysis after centrifugation if target proteins have little connection with genomic DNA. When detecting target proteins related closely to genomic DNA, sonicate gel complex and then centrifuge to collect supernatant for further analysis. Common transcription factors such as NFKB, p53 can be detected without sonication.

RIPA Lysis Buffer Images

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RIPA Lysis Buffer
RIPA Lysis Buffer (AR0105)
50 mL per pack, Solution (liquid)
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Publications

Semi-Mechanism-Based Pharmacodynamic Model for the Anti-Inflammatory Effect of Baicalein in LPS-Stimulated RAW264.7 Macrophages
Osthole Protects against Acute Lung Injury by Suppressing NF-?B-Dependent Inflammation
Icariin Activates Autophagy via Down-Regulation of the NF-?B Signaling-Mediated Apoptosis in Chondrocytes
Effect of dexmedetomidine, midazolam, and propofol on lipopolysaccharide-stimulated dendritic cells
Exogenous glutathione contributes to cisplatin resistance in lung cancer A549 cells
Neuroprotective effects of dexmedetomidine on traumatic brain injury: Involvement of neuronal apoptosis and HSP70 expression
Trichomonas vaginalis Induces Production of Proinflammatory Cytokines in Mouse Macrophages Through Activation of MAPK and NF-?B Pathways Partially Mediated by TLR2
Liraglutide Activates the Nrf2/HO-1 Antioxidant Pathway and Protects Brain Nerve Cells against Cerebral Ischemia in Diabetic Rats
Endoplasmic Reticulum Stress in Spinal Cord Contributes to the Development of Morphine Tolerance
Effect of CLIC1 gene silencing on proliferation, migration, invasion and apoptosis of human gallbladder cancer cells
Icariin Regulates the Bidirectional Differentiation of Bone Marrow Mesenchymal Stem Cells through Canonical Wnt Signaling Pathway
Loss of NDRG2 in liver microenvironment inhibits cancer liver metastasis by regulating tumor associate macrophages polarization
The inhibitory roles of Ihh downregulation on chondrocyte growth and differentiation.
Inhibition of nuclear factor kappa B pathway protects myocardial ischemia/reperfusion injury in rats under treatment with abnormal savda munziq
X-inactive-specific transcript of peripheral blood cells is regulated by exosomal Jpx and acts as a biomarker for female patients with hepatocellular carcinoma
MiR-152 Regulates Apoptosis and Triglyceride Production in MECs via Targeting ACAA2 and HSD17B12 Genes
mTOR inhibition improves the immunomodulatory properties of human bone marrow mesenchymal stem cells by inducing COX-2 and PGE2
Peli1 Contributions in Microglial Activation, Neuroinflammatory Responses and Neurological Deficits Following Experimental Subarachnoid Hemorrhage
Effects of vaspin on pancreatic ? cell secretion via PI3K/Akt and NF-?B signaling pathways
Long noncoding RNA CCAT1 functions as a ceRNA to antagonize the effect of miR-410 on the down-regulation of ITPKB in human HCT-116 and HCT-8 cells
Transcriptome sequencing of the naked mole rat (Heterocephalus glaber) and identification of hypoxia tolerance genes
Changes of Foxo3a in PBMCs and its associations with stress hyperglycemia in acute obstructive suppurative cholangitis patients
Inhibition of MD2?dependent inflammation attenuates the progression of non?alcoholic fatty liver disease
Meclizine Prevents Ovariectomy-Induced Bone Loss and Inhibits Osteoclastogenesis Partially by Upregulating PXR
Knockdown of Indian hedgehog protein induces an inhibition of cell growth and differentiation in osteoblast MC3T3-E1 cells
Iguratimod prevents ovariectomy-induced bone loss and suppresses osteoclastogenesis via inhibition of peroxisome proliferator-activated receptor-?
Nrf2/HO-1 mediates the neuroprotective effect of mangiferin on early brain injury after subarachnoid hemorrhage by attenuating mitochondria-related apoptosis and neuroinflammation
Andrographolide Inhibits Inflammatory Cytokines Secretion in LPS-Stimulated RAW264.7 Cells through Suppression of NF-?B/MAPK Signaling Pathway
Rho?kinase inhibitor Y?27632 facilitates the proliferation, migration and pluripotency of human periodontal ligament stem cells
Erythropoietin facilitates the recruitment of bone marrow mesenchymal stem cells to sites of spinal cord injury
All-trans-retinoic acid activates SDF-1/CXCR4/ROCK2 signaling pathway to inhibit chondrogenesis
Astragalus polysaccharide ameliorates H2O2-induced human umbilical vein endothelial cell injury
Effect of hypoxia-inducible factor-1/vascular endothelial growth factor signaling pathway on spinal cord injury in rats
HIV-1 Tat inhibits EAAT-2 through AEG-1 upregulation in models of HIV-associated neurocognitive disorder
Autophagy impacts on oxaliplatin-induced hepatocarcinoma apoptosis via the IL-17/IL-17R-JAK2/STAT3 signaling pathway
Inhibition of cerebral ischemia/reperfusion injury-induced apoptosis: nicotiflorin and JAK2/STAT3 pathway
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Menin mediates Tat-induced neuronal apoptosis in brain frontal cortex of SIV-infected macaques and in Tat-treated cells
Astaxanthin protects against early burn-wound progression in rats by attenuating oxidative stress-induced inflammation and mitochondria-related apoptosis
Costunolide and dehydrocostuslactone combination treatment inhibit breast cancer by inducing cell cycle arrest and apoptosis through c-Myc/p53 and AKT/14-3-3 pathway
Astaxanthin attenuates total body irradiation-induced hematopoietic system injury in mice via inhibition of oxidative stress and apoptosis
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Polyethyleneimine-coated quantum dots for miRNA delivery and its enhanced suppression in HepG2 cells
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Amarogentin Induces?Apoptosis?of Liver Cancer?Cells?via Upregulation of p53 and Downregulation of Human Telomerase Reverse Transcriptase in Mice
Fentanyl inhibits the progression of human gastric carcinoma MGC-803 cells by modulating NF-?B-dependent gene expression?in vivo
Bushen-Qiangdu-Zhilv decoction inhibits osteogenic differentiation of rat fibroblasts by regulating connexin 43
Inhibition of SDF-1?/CXCR4 Signalling in Subchondral Bone Attenuates Post-Traumatic Osteoarthritis
N-ethylmaleimide?sensitive factor siRNA inhibits the release of Weibel-Palade bodies in endothelial cells
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Autophagy protects ovarian cancer-associated fibroblasts against oxidative stress
Protection Efficacy of the Extract of Ginkgo biloba against the Learning and Memory Damage of Rats under Repeated High Sustained +Gz Exposure
Naringin Stimulates Osteogenic Differentiation of Rat Bone Marrow Stromal Cells via Activation of the Notch Signaling Pathway
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Chronic sciatic nerve compression induces fibrosis in dorsal root ganglia
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Demethylation of miR-10b plays a suppressive role in ccRCC cells
HOXB1 Is a Tumor Suppressor Gene Regulated by miR-3175 in Glioma
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Amlexanox Suppresses Osteoclastogenesis and Prevents Ovariectomy-Induced Bone Loss
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Epigenetic silencing of olfactomedin-4 enhances gastric cancer cell invasion via activation of focal adhesion kinase signaling
Autologous bone marrow mononuclear cell transplantation delays progression of carotid atherosclerosis in rabbits
Rhizoma Paridis Saponins Suppresses Tumor Growth in a Rat Model of N-Nitrosomethylbenzylamine-Induced Esophageal Cancer by Inhibiting Cyclooxygenases-2 Pathway
Nanog down-regulates the Wnt signaling pathway via ?-catenin phosphorylation during epidermal stem cell proliferation and differentiation
Beneficial Effects of Hydrogen-Rich Saline on Early Burn-Wound Progression in Rats
Astaxanthin Attenuates Early Acute Kidney Injury Following Severe Burns in Rats by Ameliorating Oxidative Stress and Mitochondrial-Related Apoptosis
Differential Expression of miR-499 and Validation of Predicted Target Genes in the Testicular Tissue of Swine at Different Developmental Stages
Epigenetic reprogramming of human lung cancer cells with the extract of bovine parthenogenetic oocytes
Function of the transforming growth factor-?1/c-Jun N-terminal kinase signaling pathway in the action of thalidomide on a rat model of pulmonary fibrosis
c?Ski activates cancer?associated fibroblasts to regulate breast cancer cell invasion
The effects of ERCC1 expression levels on the chemosensitivity of gastric cancer cells to platinum agents and survival in gastric cancer patients treated with oxaliplatin-based adjuvant chemotherapy
A novel DNA vaccine expressing the Ag85A-HA2 fusion protein provides protection against influenza A virus and?Staphylococcus aureus
Effects of Extract from Mangifera indica Leaf on Monosodium Urate Crystal-Induced Gouty Arthritis in Rats
PLK1 gene suppresses cell invasion of undifferentiated thyroid carcinoma through the inhibition of CD44v6, MMP-2 and MMP-9
siRNA targeting decoy receptor 3 enhances the sensitivity of gastric carcinoma cells to 5-fluorouracil
A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus).
Effect of Buddleia flavonoids drug-containing plasma on the expression of STAT1 phosphoprotein in lacrimal gland epithelial cells in vitro
A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus).
Effect of Buddleia flavonoids drug-containing plasma on the expression of STAT1 phosphoprotein in lacrimal gland epithelial cells in vitro
Deng A, Zhang H, Hu M, Liu S, Gao Q, Wang Y, Guo C. Mol Med Rep. 2017 Dec;16(6):7987-7992. doi: 10.3892/mmr.2017.7669. Epub 2017 Sep 29. Knockdown of Indian hedgehog protein induces an inhibition of cell growth and differentiation in osteoblast MC3T3-E1 cells
Ma X, Yuan T, Yang C, Wang Z, Zang Y, Wu L, Zhuang L. Ther Adv Med Oncol. 2017 Nov;9(11):665-677. doi: 10.1177/1758834017731052. Epub 2017 Sep 21. X-inactive-specific transcript of peripheral blood cells is regulated by exosomal Jpx and acts as a biomarker for female patients with hepatocellular carcinoma
Zhang Y, Wu B, Zhang H, Ge X, Ying S, Hu M, Li W, Huang Y, Wang L, Chen C, Shan X, Liang G. J Cell Mol Med. 2018 Feb;22(2):936-947. doi: 10.1111/jcmm.13395. Epub 2017 Oct 27. Inhibition of MD2
Deng A, Zhang H, Hu M, Liu S, Wang Y, Gao Q, Guo C. Exp Ther Med. 2018 Jan;15(1):789-794. doi: 10.3892/etm.2017.5458. Epub 2017 Nov 7. The inhibitory roles of Ihh downregulation on chondrocyte growth and differentiation.
Li L, Li X, Gong P, Zhang X, Yang Z, Yang J, Li J. Front Microbiol. 2018 Apr 10;9:712. doi: 10.3389/fmicb.2018.00712. eCollection 2018. Trichomonas vaginalis Induces Production of Proinflammatory Cytokines in Mouse Macrophages Through Activation of MAPK and NF-κB Pathways Partially Mediated by TLR2
He YM, Zhang ZL, Liu QY, Xiao YS, Wei L, Xi C, Nan X. J Cell Mol Med. 2018 May;22(5):2569-2579. doi: 10.1111/jcmm.13499. Epub 2018 Mar 8. Effect of CLIC1 gene silencing on proliferation, migration, invasion and apoptosis of human gallbladder cancer cells
Huang XP, Peng JH, Pang JW, Tian XC, Li XS, Wu Y, Li Y, Jiang Y, Sun XC. Front Mol Neurosci. 2017 Nov 30;10:398. doi: 10.3389/fnmol.2017.00398. eCollection 2017. Peli1 Contributions in Microglial Activation, Neuroinflammatory Responses and Neurological Deficits Following Experimental Subarachnoid Hemorrhage
Wang B, Lin Y, Hu Y, Shan W, Liu S, Xu Y, Zhang H, Cai S, Yu X, Cai Z, Huang H. Stem Cell Res Ther. 2017 Dec 29;8(1):292. doi: 10.1186/s13287-017-0744-6. mTOR inhibition improves the immunomodulatory properties of human bone marrow mesenchymal stem cells by inducing COX-2 and PGE2
Li B, Shi C, Zhao J, Li B. Oncotarget. 2017 Oct 7;8(54):92855-92863. doi: 10.18632/oncotarget.21612. eCollection 2017 Nov 3. Long noncoding RNA CCAT1 functions as a ceRNA to antagonize the effect of miR-410 on the down-regulation of ITPKB in human HCT-116 and HCT-8 cells
Liu S, Li X, Wu Y, Duan R, Zhang J, Du F, Zhang Q, Li Y, Li N. PLoS One. 2017 Dec 14;12(12):e0189722. doi: 10.1371/journal.pone.0189722. eCollection 2017. Effects of vaspin on pancreatic β cell secretion via PI3K/Akt and NF-κB signaling pathways
Wu YX, Sun Y, Ye YP, Zhang P, Guo JC, Huang JM, Jing XZ, Xiang W, Yu SY, Guo FJ. Mol Med Rep. 2017 Dec;16(6):8200-8208. doi: 10.3892/mmr.2017.7648. Epub 2017 Sep 28. Iguratimod prevents ovariectomy-induced bone loss and suppresses osteoclastogenesis via inhibition of peroxisome proliferator-activated receptor-γ
Huang JM, Bao Y, Xiang W, Jing XZ, Guo JC, Yao XD, Wang R, Guo FJ. Evid Based Complement Alternat Med. 2017;2017:8085325. doi: 10.1155/2017/8085325. Epub 2017 Dec 27. Icariin Regulates the Bidirectional Differentiation of Bone Marrow Mesenchymal Stem Cells through Canonical Wnt Signaling Pathway
Guo J, Li W, Wu Y, Jing X, Huang J, Zhang J, Xiang W, Ren R, Lv Z, Xiao J, Guo F. Front Pharmacol. 2017 Oct 4;8:693. doi: 10.3389/fphar.2017.00693. eCollection 2017. Meclizine Prevents Ovariectomy-Induced Bone Loss and Inhibits Osteoclastogenesis Partially by Upregulating PXR
Li Y, He S, Tang J, Ding N, Chu X, Cheng L, Ding X, Liang T, Feng S, Rahman SU, Wang X, Wu J. Andrographolide Inhibits Inflammatory Cytokines Secretion in LPS-Stimulated RAW264.7 Cells through Suppression of NF-κB/MAPK Signaling Pathway
Xiao B, Li L, Xu C, Zhao S, Lin L, Cheng J, Yang W, Cong W, Kan G, Cui S. Biol Open. 2017 Dec 15;6(12):1904-1912. doi: 10.1242/bio.028548. Transcriptome sequencing of the naked mole rat (Heterocephalus glaber) and identification of hypoxia tolerance genes
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Chen H, Li J, Liang S, Lin B, Peng Q, Zhao P, Cui J, Rao Y. Exp Ther Med. 2017 Mar;13(3):861-866. doi: 10.3892/etm.2017.4049. Epub 2017 Jan 16. Effect of hypoxia-inducible factor-1/vascular endothelial growth factor signaling pathway on spinal cord injury in rats
Peng Z, Wang Y, Fan J, Lin X, Liu C, Xu Y, Ji W, Yan C, Su C. Sci Rep. 2017 Jan 24;7:41254. doi: 10.1038/srep41254. Costunolide and dehydrocostuslactone combination treatment inhibit breast cancer by inducing cell cycle arrest and apoptosis through c-Myc/p53 and AKT/14-3-3 pathway
Fang Q, Guo S, Zhou H, Han R, Wu P, Han C. Sci Rep. 2017 Jan 27;7:41440. doi: 10.1038/srep41440. Astaxanthin protects against early burn-wound progression in rats by attenuating oxidative stress-induced inflammation and mitochondria-related apoptosis
Hu GQ, Du X, Li YJ, Gao XQ, Chen BQ, Yu L. Neural Regen Res. 2017 Jan;12(1):96-102. doi: 10.4103/1673-5374.198992. Inhibition of cerebral ischemia/reperfusion injury-induced apoptosis: nicotiflorin and JAK2/STAT3 pathway
Liu D, Zhou Y, Peng Y, Su P, Li Z, Xu Q, Tu Y, Tian X, Yang H, Wu Z, Mei W, Gao F. Front Mol Neurosci. 2018 Mar 6;11:72. doi: 10.3389/fnmol.2018.00072. eCollection 2018. Endoplasmic Reticulum Stress in Spinal Cord Contributes to the Development of Morphine Tolerance
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Maimaitiaili A, Li J, Aibibula A, Abudureheman M. Am J Transl Res. 2018 Jan 15;10(1):77-85. eCollection 2018. Inhibition of nuclear factor kappa B pathway protects myocardial ischemia/reperfusion injury in rats under treatment with abnormal savda munziq
Tian, J., Liu, J., & Liang, J. (2017). HBx enhances cell viability, migration and invasion of hepatocellular carcinoma via PD-L1. Cancer Cell Research, 4(14), 351-355. Retrieved from http://www.cancercellresearch.org/PDF/20171405.pdf
Hu, Q.X., Li, X.D., Xie, P., Wu, C.C., Zheng, G.Z., Lin, F.X.,…, & Du, S.X. (2017). All-trans-retinoic acid activates SDF-1/CXCR4/ROCK2 signaling pathway to inhibit chondrogenesis. American Journal of Translational Research, 9(5), 2296-2305. Retrieved from http://www.ajtr.org/files/ajtr0048341.pdf
Liu, X., Guo, J., Zhou, M., Yang, Y., Liang, M., Bai, C., Zhao, Z., & Sun, B. (2017). RNA binding protein QKI contributes to WT1 mRNA and suppresses apoptosis in ST cells. Genes & Genomics, 1-8. doi:10.1007/s13258-017-0560-5
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Lv J, Sun X, Ma J, Ma X, Zhang Y, Li F, Li Y, Zhao Z. Biochem Biophys Res Commun. 2015 Aug 14;464(1):263-8. Doi: 10.1016/J.Bbrc.2015.06.140. Epub 2015 Jun 23. Netrin-1 Induces The Migration Of Schwann Cells Via P38 Mapk And Pi3K-Akt Signaling Path...
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Customer Q&As

Q: Can I use this buffer for tissue lysate preparation in ELISA? Keywords: protocol, alternative buffer, applications
A: Yes, RIPA lysis buffer can be used for tissue lysate preparation in ELISA.
Q: What is the RIPA Lysis Buffer (SKU: AR0105 ) composed of? Keyword: ingredients, composition
A: It is composed of SDS, NP-40, Sodium deoxycholate and 0.02% Sodium azide.