Show Order Info
SKU AR0105
Pack size 50ml
Form Solution (liquid)
Applications Western blotting, ELISA, immunofluorescence, Immunoprecipitation; protein purification; SDS-PAGE; protein assays; epitope tagging; reporter gene analysis

Overview

Product Name RIPA Lysis Buffer
Synonyms RIPA buffer; Radio-immunoprecipitation assay buffer, RIPA Lysis and Extraction Buffer
Description RIPA Lysis Buffer is a complete cell lysis solution reagent used for rapid and efficient total cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells, effectively extracting cytoplasmic, nuclear and membrane proteins. RIPA lysis buffer is highly compatible with various downstream protein analysis applications.
Application Western blotting, ELISA, immunofluorescence, Immunoprecipitation; protein purification; SDS-PAGE; assays for protein expression, activity, modification, profiling, characterization and quantitative measurement; epitope tagging; reporter gene analysis
Pack Size 50 ml
Reagent Type Western Blotting related reagent; Cell lysis buffer; Universal tissue extraction buffer; Detergent solution
Content 1% NP-40, 0.5% Sodium deoxycholate, 0.1% SDS
Usage Extraction of cytoplasmic, membrane and nuclear proteins

Properties

Form Supplied Ready-to-use 1X solution
Physical State Liquid
Pack Size 50 ml
Recommended working concentration 10 ml RIPA Lysis Buffer per gram of tissue
0.5 ml RIPA Lysis Buffer per 5.0x106 cells in suspension
0.5 ml RIPA Lysis Buffer per 5.0x106 adherent mammalian cells
0.5-1 ml RIPA lysis buffer per subconfluent monolayer on a 100 mm plate
Compatibility with reagents Fully compatible with Broad Spectrum Protease Inhibitor Cocktail and Broad Spectrum Phosphatase Inhibitor Cocktail
Storage Store at 4˚C for one year.

Chemical Information

Component: Effects:
NP-40 Non-ionic detergent to extract proteins
Sodium deoxycholate Ionic detergent to extract membrane proteins
SDS Ionic detergents to extract proteins

Usage and Handling

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. RIPA lysis buffer is highly compatible with immunoassays, protein purification procedures, immunoprecipitation, and western blotting.

Background

RIPA lysis extraction buffer contains non-ionic and ionic detergents which are able to extract protein from wide variety of cell types and membrane structures. RIPA buffer ensures efficient cell lysis and protein solubilization preventing protein degradation and interference with protein immunoreactivity and biological activity. Since most antibodies and protein antigens are not adversely affected by the components of this solution, RIPA buffer-conducted protein extraction is compatible with various downstream immunoprecipitation and molecular pull-down assays. RIPA buffer reagent minimizes non-specific protein-binding interactions to keep background low, while allowing most specific interactions to occur, enabling studies of relevant protein-protein interactions.

RIPA Lysis Buffer Images

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RIPA Lysis Buffer
RIPA Lysis Buffer (AR0105)
50 mL per pack, Solution (liquid)
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Publications

Deng A, Zhang H, Hu M, Liu S, Gao Q, Wang Y, Guo C. Mol Med Rep. 2017 Dec;16(6):7987-7992. doi: 10.3892/mmr.2017.7669. Epub 2017 Sep 29. Knockdown of Indian hedgehog protein induces an inhibition of cell growth and differentiation in osteoblast MC3T3-E1 cells
Ma X, Yuan T, Yang C, Wang Z, Zang Y, Wu L, Zhuang L. Ther Adv Med Oncol. 2017 Nov;9(11):665-677. doi: 10.1177/1758834017731052. Epub 2017 Sep 21. X-inactive-specific transcript of peripheral blood cells is regulated by exosomal Jpx and acts as a biomarker for female patients with hepatocellular carcinoma
Zhang Y, Wu B, Zhang H, Ge X, Ying S, Hu M, Li W, Huang Y, Wang L, Chen C, Shan X, Liang G. J Cell Mol Med. 2018 Feb;22(2):936-947. doi: 10.1111/jcmm.13395. Epub 2017 Oct 27. Inhibition of MD2
Deng A, Zhang H, Hu M, Liu S, Wang Y, Gao Q, Guo C. Exp Ther Med. 2018 Jan;15(1):789-794. doi: 10.3892/etm.2017.5458. Epub 2017 Nov 7. The inhibitory roles of Ihh downregulation on chondrocyte growth and differentiation.
Li L, Li X, Gong P, Zhang X, Yang Z, Yang J, Li J. Front Microbiol. 2018 Apr 10;9:712. doi: 10.3389/fmicb.2018.00712. eCollection 2018. Trichomonas vaginalis Induces Production of Proinflammatory Cytokines in Mouse Macrophages Through Activation of MAPK and NF-κB Pathways Partially Mediated by TLR2
He YM, Zhang ZL, Liu QY, Xiao YS, Wei L, Xi C, Nan X. J Cell Mol Med. 2018 May;22(5):2569-2579. doi: 10.1111/jcmm.13499. Epub 2018 Mar 8. Effect of CLIC1 gene silencing on proliferation, migration, invasion and apoptosis of human gallbladder cancer cells
Huang XP, Peng JH, Pang JW, Tian XC, Li XS, Wu Y, Li Y, Jiang Y, Sun XC. Front Mol Neurosci. 2017 Nov 30;10:398. doi: 10.3389/fnmol.2017.00398. eCollection 2017. Peli1 Contributions in Microglial Activation, Neuroinflammatory Responses and Neurological Deficits Following Experimental Subarachnoid Hemorrhage
Wang B, Lin Y, Hu Y, Shan W, Liu S, Xu Y, Zhang H, Cai S, Yu X, Cai Z, Huang H. Stem Cell Res Ther. 2017 Dec 29;8(1):292. doi: 10.1186/s13287-017-0744-6. mTOR inhibition improves the immunomodulatory properties of human bone marrow mesenchymal stem cells by inducing COX-2 and PGE2
Li B, Shi C, Zhao J, Li B. Oncotarget. 2017 Oct 7;8(54):92855-92863. doi: 10.18632/oncotarget.21612. eCollection 2017 Nov 3. Long noncoding RNA CCAT1 functions as a ceRNA to antagonize the effect of miR-410 on the down-regulation of ITPKB in human HCT-116 and HCT-8 cells
Liu S, Li X, Wu Y, Duan R, Zhang J, Du F, Zhang Q, Li Y, Li N. PLoS One. 2017 Dec 14;12(12):e0189722. doi: 10.1371/journal.pone.0189722. eCollection 2017. Effects of vaspin on pancreatic β cell secretion via PI3K/Akt and NF-κB signaling pathways
Wu YX, Sun Y, Ye YP, Zhang P, Guo JC, Huang JM, Jing XZ, Xiang W, Yu SY, Guo FJ. Mol Med Rep. 2017 Dec;16(6):8200-8208. doi: 10.3892/mmr.2017.7648. Epub 2017 Sep 28. Iguratimod prevents ovariectomy-induced bone loss and suppresses osteoclastogenesis via inhibition of peroxisome proliferator-activated receptor-γ
Huang JM, Bao Y, Xiang W, Jing XZ, Guo JC, Yao XD, Wang R, Guo FJ. Evid Based Complement Alternat Med. 2017;2017:8085325. doi: 10.1155/2017/8085325. Epub 2017 Dec 27. Icariin Regulates the Bidirectional Differentiation of Bone Marrow Mesenchymal Stem Cells through Canonical Wnt Signaling Pathway
Guo J, Li W, Wu Y, Jing X, Huang J, Zhang J, Xiang W, Ren R, Lv Z, Xiao J, Guo F. Front Pharmacol. 2017 Oct 4;8:693. doi: 10.3389/fphar.2017.00693. eCollection 2017. Meclizine Prevents Ovariectomy-Induced Bone Loss and Inhibits Osteoclastogenesis Partially by Upregulating PXR
Li Y, He S, Tang J, Ding N, Chu X, Cheng L, Ding X, Liang T, Feng S, Rahman SU, Wang X, Wu J. Andrographolide Inhibits Inflammatory Cytokines Secretion in LPS-Stimulated RAW264.7 Cells through Suppression of NF-κB/MAPK Signaling Pathway
Xiao B, Li L, Xu C, Zhao S, Lin L, Cheng J, Yang W, Cong W, Kan G, Cui S. Biol Open. 2017 Dec 15;6(12):1904-1912. doi: 10.1242/bio.028548. Transcriptome sequencing of the naked mole rat (Heterocephalus glaber) and identification of hypoxia tolerance genes
Bailin N, Nan C, Peizhi L, Kun H, Xiwen Z, Guosheng R, Jianping G, Wenfeng Z. Oncotarget. 2017 Aug 7;8(44):76783-76796. doi: 10.18632/oncotarget.20011. eCollection 2017 Sep 29. Changes of Foxo3a in PBMCs and its associations with stress hyperglycemia in acute obstructive suppurative cholangitis patients
Chen H, Li J, Liang S, Lin B, Peng Q, Zhao P, Cui J, Rao Y. Exp Ther Med. 2017 Mar;13(3):861-866. doi: 10.3892/etm.2017.4049. Epub 2017 Jan 16. Effect of hypoxia-inducible factor-1/vascular endothelial growth factor signaling pathway on spinal cord injury in rats
Peng Z, Wang Y, Fan J, Lin X, Liu C, Xu Y, Ji W, Yan C, Su C. Sci Rep. 2017 Jan 24;7:41254. doi: 10.1038/srep41254. Costunolide and dehydrocostuslactone combination treatment inhibit breast cancer by inducing cell cycle arrest and apoptosis through c-Myc/p53 and AKT/14-3-3 pathway
Fang Q, Guo S, Zhou H, Han R, Wu P, Han C. Sci Rep. 2017 Jan 27;7:41440. doi: 10.1038/srep41440. Astaxanthin protects against early burn-wound progression in rats by attenuating oxidative stress-induced inflammation and mitochondria-related apoptosis
Hu GQ, Du X, Li YJ, Gao XQ, Chen BQ, Yu L. Neural Regen Res. 2017 Jan;12(1):96-102. doi: 10.4103/1673-5374.198992. Inhibition of cerebral ischemia/reperfusion injury-induced apoptosis: nicotiflorin and JAK2/STAT3 pathway
Liu D, Zhou Y, Peng Y, Su P, Li Z, Xu Q, Tu Y, Tian X, Yang H, Wu Z, Mei W, Gao F. Front Mol Neurosci. 2018 Mar 6;11:72. doi: 10.3389/fnmol.2018.00072. eCollection 2018. Endoplasmic Reticulum Stress in Spinal Cord Contributes to the Development of Morphine Tolerance
Deng C, Cao J, Han J, Li J, Li Z, Shi N, He J. Comput Intell Neurosci. 2018 Feb 12;2018:3094504. doi: 10.1155/2018/3094504. eCollection 2018. Liraglutide Activates the Nrf2/HO-1 Antioxidant Pathway and Protects Brain Nerve Cells against Cerebral Ischemia in Diabetic Rats
Li M, Lai X, Zhao Y, Zhang Y, Li M, Li D, Kong J, Zhang Y, Jing P, Li H, Qin H, Shen L, Yao L, Li J, Dou K0, Zhang J. Cell Death Dis. 2018 Feb 14;9(2):248. doi: 10.1038/s41419-018-0284-8. Loss of NDRG2 in liver microenvironment inhibits cancer liver metastasis by regulating tumor associate macrophages polarization
Yang Y, Fang X, Yang R, Yu H, Jiang P, Sun B, Zhao Z. Sci Rep. 2018 Jan 11;8(1):417. doi: 10.1038/s41598-017-18804-x. MiR-152 Regulates Apoptosis and Triglyceride Production in MECs via Targeting ACAA2 and HSD17B12 Genes
Maimaitiaili A, Li J, Aibibula A, Abudureheman M. Am J Transl Res. 2018 Jan 15;10(1):77-85. eCollection 2018. Inhibition of nuclear factor kappa B pathway protects myocardial ischemia/reperfusion injury in rats under treatment with abnormal savda munziq
Tian, J., Liu, J., & Liang, J. (2017). HBx enhances cell viability, migration and invasion of hepatocellular carcinoma via PD-L1. Cancer Cell Research, 4(14), 351-355. Retrieved from http://www.cancercellresearch.org/PDF/20171405.pdf
Hu, Q.X., Li, X.D., Xie, P., Wu, C.C., Zheng, G.Z., Lin, F.X.,…, & Du, S.X. (2017). All-trans-retinoic acid activates SDF-1/CXCR4/ROCK2 signaling pathway to inhibit chondrogenesis. American Journal of Translational Research, 9(5), 2296-2305. Retrieved from http://www.ajtr.org/files/ajtr0048341.pdf
Liu, X., Guo, J., Zhou, M., Yang, Y., Liang, M., Bai, C., Zhao, Z., & Sun, B. (2017). RNA binding protein QKI contributes to WT1 mRNA and suppresses apoptosis in ST cells. Genes & Genomics, 1-8. doi:10.1007/s13258-017-0560-5
Xiao-Lei Xue, Xiao-Dan Han, Yuan Li, Xiao-Fei Chu, Wei-Min Miao, Jun-Ling ZhangEmail author and Sai-Jun FanEmail author Stem Cell Research & Therapy20178:7 DOI: 10.1186/s13287-016-0464-3 Astaxanthin attenuates total body irradiation-induced hematopoietic system injury in mice via inhibition of oxidative stress and apoptosis
Qian Yu, Minchao Li Journal of Biomechanics Available online 9 December 2016 Effects of transient receptor potential canonical 1 (TRPC1) on the mechanical stretch-induced expression of airway remodeling-associated factors in human bronchial epithelioid cells
Gaofeng Liang, Yang Li, Wenpo Feng, Xinshuai Wang, Aihua Jing, Jinghua Li, and Kaiwang Ma Int J Nanomedicine. 2016; 11: 6079–6088. Published online 2016 Nov 15. doi: 10.2147/IJN.S120828 Polyethyleneimine-coated quantum dots for miRNA delivery and its enhanced suppression in HepG2 cells
Tang W, Yang J, Zhang F, Guo H, Peng F, Wang X. Cytotherapy. 2014 Feb;16(2):225-33. Doi: 10.1016/J.Jcyt.2013.08.009. Epub 2013 Oct 8. Activation Of Extracellular Signal-Regulated Kinase 1/2 And Sp1 May Contribute To The Expression Of Tissue Inhibi...
Chen Z, Ma T, Huang C, Zhang L, Zhong J, Han J, Hu T, Li J. Eur J Pharm Sci. 2014 Mar 12;53:69-76. Doi: 10.1016/J.Ejps.2013.12.010. Epub 2013 Dec 21. Efficiency Of Transcellular Transport And Efflux Of Flavonoids With Different Glycosidic Units Fr...
Xiao W, Gao Z, Duan Y, Yuan W, Ke Y. Oncol Rep. 2015 Aug;34(2):739-46. Doi: 10.3892/Or.2015.4041. Epub 2015 Jun 8. Downregulation Of Mir-19A Exhibits Inhibitory Effects On Metastatic Renal Cell Carcinoma By Targeting Pik3Ca And Inactivating Notch ...
Sun Y, Chen H, Ma S, Liang L, Zheng Y, Guo X, Wang M, Wang W, Li G, Zhong D. Neurochem Res. 2015 Jul;40(7):1410-20. Doi: 10.1007/S11064-015-1608-Z. Epub 2015 May 22. Administration Of Sb203580, A P38 Mapk Inhibitor, Reduced The Expression Of Mmp9,...
Wang J, Liao Y, Fan J, Ye T, Sun X, Dong S. Inflammation. 2012 Aug;35(4):1466-76. Doi: 10.1007/S10753-012-9460-7. Apigenin Inhibits The Expression Of Il-6, Il-8, And Icam-1 In Dehp-Stimulated Human Umbilical Vein Endothelial Cells And In Vivo.
Wang Z, Dao R, Bao L, Dong Y, Wang H, Han P, Yue Y, Yu H. J Cell Mol Med. 2014 Sep;18(9):1807-15. Doi: 10.1111/Jcmm.12306. Epub 2014 May 30. Epigenetic Reprogramming Of Human Lung Cancer Cells With The Extract Of Bovine Parthenogenetic Oocytes.
Lv J, Sun X, Ma J, Ma X, Zhang Y, Li F, Li Y, Zhao Z. Biochem Biophys Res Commun. 2015 Aug 14;464(1):263-8. Doi: 10.1016/J.Bbrc.2015.06.140. Epub 2015 Jun 23. Netrin-1 Induces The Migration Of Schwann Cells Via P38 Mapk And Pi3K-Akt Signaling Path...
Dong L, Wang X, Tan J, Li H, Qian W, Chen J, Chen Q, Wang J, Xu W, Tao C, Wang S. J Cell Mol Med. 2014 Nov;18(11):2213-24. Doi: 10.1111/Jcmm.12353. Epub 2014 Aug 28. Decreased Expression Of Microrna-21 Correlates With The Imbalance Of Th17 And Tre...
Wang Yg, Xie P, Wang Yg, Li Xd, Zhang Tg, Liu Zy, Hong Q, Du Sx. Environ Toxicol Pharmacol. 2014 Sep;38(2):460-8. Doi: 10.1016/J.Etap.2014.07.008. Epub 2014 Jul 21. All-Trans-Retinoid Acid (Atra) Suppresses Chondrogenesis Of Rat Primary Hind Limb ...
Guo Sx, Jin Yy, Fang Q, You Cg, Wang Xg, Hu Xl, Han Cm. Plos One. 2015 Apr 13;10(4):E0124897. Doi: 10.1371/Journal.Pone.0124897. Ecollection 2015. Beneficial Effects Of Hydrogen-Rich Saline On Early Burn-Wound Progression In Rats.
Zhou J, Qu C, Sun Q, Wu L, Liu Y, Yang Z, Zhang J. Chem Biol Interact. 2014 Aug 5;219:57-63. Doi: 10.1016/J.Cbi.2014.05.009. Epub 2014 May 28. Sophoricoside Fails The Embryo Implantation By Compromising The Uterine Endometrial Receptivity At Impla...
Lyu Z, Mao Z, Wang H, Fang Y, Chen T, Wan Q, Wang M, Wang N, Xiao J, Wei H, Li X, Liu Y, Zhou Q. Biochem Biophys Res Commun. 2013 Nov 1;440(4):495-501. Doi: 10.1016/J.Bbrc.2013.09.059. Epub 2013 Sep 20. Mir-181B Targets Six2 And Inhibits The Proli...
Huang W, Lu C, Wu Y, Ouyang S, Chen Y. Biochem Biophys Res Commun. 2015 Mar 6;458(2):294-9. Doi: 10.1016/J.Bbrc.2015.01.103. Epub 2015 Jan 31. Identification And Functional Characterization Of Voltage-Gated Sodium Channels In Lymphocytes.

Customer Q&As

Q: Can I use this buffer for tissue lysate preparation in ELISA? Keywords: protocol, alternative buffer, applications
A: Yes, RIPA lysis buffer can be used for tissue lysate preparation in ELISA.
Q: What is the RIPA Lysis Buffer (SKU: AR0105 ) composed of? Keyword: ingredients, composition
A: It is composed of SDS, NP-40, Sodium deoxycholate and 0.02% Sodium azide.