Deliver Custom DNA Quickly with our custom DNA synthesis platform

Custom Gene Synthesis Service

Precision DNA synthesis with guaranteed 100% sequence accuracy, optimized for expression and delivered ready-to-use in your vector of choice

Fast Turnaround
From 5 business days

100% Accuracy
Sequence-verified genes

Competitive Pricing
Starting at $0.20/base

Begin Inquiry

Gene Synthesis Options Matched to Your Project

Choose from standard, complex, or high-throughput gene synthesis services tailored to your specific research requirements and timelines

Standard Gene Synthesis

Ideal for routine cloning projects up to 12 kb. Fast turnaround with proven reliability for most protein-coding sequences and standard constructs.

Gene Length	Base Price	Non-Complex Turnaround	Complex Turnaround
100-1,000 bp	$0.20/bp (min $50)	5-10 business days	12-15 business days
1,001-3,000 bp	$0.21/bp	12-15 business days	15-20 business days
3,001-5,000 bp	$0.22/bp	15-20 business days	20-25 business days
5,001-7,000 bp	Custom quote	As quoted	As quoted
7,001-12,000 bp	Custom quote	As quoted	As quoted

What's Included:

Free codon optimization using advanced algorithms
100% sequence verification by Sanger sequencing
Cloning into your vector of choice or standard shuttle vector
5 µg plasmid DNA vacuum-dried and ready to resuspend
Free vector storage for future orders

Complex Sequence Synthesis

Specialized synthesis for challenging sequences with high GC content, repetitive regions, secondary structures, or sequences over 12 kb requiring expert handling.

Request Quote

Rush Service

Express delivery options available for time-sensitive projects. SuperSPEED service delivers genes up to 1.2 kb in just 5 business days.

Request Quote

Flexible Delivery Formats for Your Lab Setup

Receive your synthesized genes in the format that best suits your workflow

Vector Formats

Standard Cloning Vectors

Choose from our extensive library of expression vectors for bacterial, yeast, insect, and mammalian systems, comparable in breadth to a typical GenScript plasmid list used by many researchers. All major promoters and tags available.

pUC-based shuttle vectors
T7 expression vectors
CMV mammalian expression vectors
Yeast 2-hybrid vectors
Gateway-compatible vectors

Custom Vector Onboarding

Need your gene in a proprietary vector? We offer custom vector onboarding services that effectively function as a custom plasmid synthesis solution tailored to your constructs. Your vector remains confidential and exclusively available for your future orders.

DNA Formats

Standard Delivery

Plasmid DNA: 5 µg vacuum-dried in 1.5 mL tubes
Linear DNA fragments: PCR-ready format
Gene fragments: Optimized for assembly methods

High-Throughput Options

96-well plate format: For screening projects
384-well plate format: For library construction
Pooled libraries: Custom complexity

Scaled-Up Preparations

Need more DNA? Add scaled-up plasmid preparation services for ready-to-use quantities up to 10 mg, with clear guidelines comparable to a typical GenScript plasmid resuspension protocol.

Advanced Features and Capabilities

Leverage our technology to optimize your gene constructs for maximum expression and stability

Codon Optimization

AI-powered codon optimization goes beyond simple codon usage to consider mRNA secondary structure, ribosome binding sites, and expression optimization for your target organism.

Complex Sequence Design

Confidently design genes with extreme GC content, long repeats, or intricate secondary structures. Our proprietary algorithms handle sequences others cannot synthesize.

No Size Limits

From short gene fragments to entire metabolic pathways and mini-genomes. We have successfully synthesized constructs exceeding 50 kb for synthetic biology applications.

Applications Across Research Areas

Our DNA synthesis services support diverse applications in molecular biology, synthetic biology, and biotechnology and are positioned alongside leading DNA synthesis companies in these fields

Research Applications

Protein expression and purification: Optimized genes for high-yield production
Antibody engineering: Custom antibody sequences and humanization
CRISPR gene editing: Guide RNA templates and Cas9 variants
Metabolic engineering: Pathway construction and optimization
Vaccine development: Antigen optimization and codon adaptation

Therapeutic Development

Gene therapy vectors: AAV, lentiviral, and adenoviral constructs
mRNA therapeutics: Optimized coding sequences
CAR-T development: Chimeric antigen receptor designs
Biologics production: Monoclonal antibodies and fusion proteins
Enzyme replacement therapy: Human-optimized enzymes

Complete Workflow Support

We provide end-to-end solutions from gene design through expression validation

1. Sequence Design & Optimization

Submit your sequence or protein sequence. Our team applies advanced optimization algorithms and checks for synthesis feasibility.
2. Vector Selection & Construct Design

Choose from our vector library or onboard your custom vector. We design the complete construct with your required elements.
3. Gene Synthesis & Cloning

Your gene is synthesized with 100% accuracy and cloned into the specified vector using our automated platform.
4. Quality Control & Sequencing

Every construct is verified by complete Sanger sequencing to ensure perfect accuracy before delivery.
5. Delivery & Expression Support (Optional)

Receive your sequence-verified plasmid ready for transformation. Add-on protein expression and purification services available.

Frequently Asked Questions

Common questions about our gene synthesis services

What is gene synthesis and how does it work?

Gene synthesis is the process of artificially creating DNA sequences in the laboratory without using a biological template and is often referred to as artificial DNA synthesis in modern molecular biology, a field that grew rapidly after the first artificial synthesis of DNA was done by pioneering molecular biologists. Our gene synthesis service uses advanced oligonucleotide synthesis technology combined with assembly methods to build your exact sequence from scratch, replacing the need for legacy instruments such as an ABI DNA synthesizer in routine workflows, so you never have to worry about a DNA synthesizer how it works or is maintained in-house. This allows you to obtain genes from any organism, create novel sequences, add specific mutations, or optimize codons for expression—all without the need for PCR amplification, a benchtop DNA synthesizer, or traditional cloning, giving you full control over how synthetic DNA is made for your experiments. The process includes automated sequence design, synthesis, error correction, assembly, and quality verification by Sanger sequencing, covering all key gene synthesis steps from design to final QC without requiring you to purchase or maintain a dedicated gene synthesis machine.

What are the essential questions to ask before choosing a gene synthesis service for my lab?

Before selecting a gene synthesis provider, consider these key factors: (1) Sequence complexity handling—Can they synthesize genes with high/low GC content, repeats, or secondary structures? (2) Turnaround time flexibility—Do they offer standard and rush services that match your project timelines? (3) Vector compatibility—Can they clone into your preferred expression vector or onboard custom vectors? (4) Quality assurance—Is full sequence verification included? (5) Pricing structure—Are costs transparent with no hidden fees for complexity? (6) Size capabilities—What is the maximum construct size they can deliver? (7) Additional services—Do they offer codon optimization, expression validation, or protein production? Our service addresses all these needs with transparent pricing, guaranteed accuracy, and comprehensive support.

What key features should I look for when purchasing gene synthesis for high-throughput projects?

For high-throughput gene synthesis projects, prioritize these features: (1) Scalability—Ability to handle 96-well, 384-well, or larger batch orders with consistent quality. (2) Automated ordering systems—Online platforms that support bulk uploads and project management. (3) Flexible delivery formats—Options for plate formats, pooled libraries, or individual tubes based on your workflow. (4) Standardized quality control—Automated sequencing verification for all constructs. (5) Batch pricing discounts—Cost advantages for large orders. (6) Vector library compatibility—Support for standard vector formats used in screening. (7) Data management—Comprehensive tracking and delivery of sequence data. Our high-throughput gene synthesis service includes all these features plus specialized support for library construction, variant generation, and synthetic biology applications.

How much does gene synthesis cost, how do DNA synthesis costs compare between providers, and what factors affect the price?

Our gene synthesis pricing starts at $0.20 per base pair with a minimum order of $50. The cost depends primarily on sequence length, with longer genes receiving per-base discounts. Other factors affecting price include: sequence complexity (GC content, repeats, secondary structure), rush service requests, and special delivery formats, whereas instrument-related expenses such as an oligo synthesizer machine price are not part of our service fees. Standard pricing includes free codon optimization, sequence verification, cloning into vector, and 5 µg of plasmid DNA. Additional services like scaled-up DNA preparation, protein expression validation, or custom vector onboarding are available at additional cost. For complex sequences over 7 kb or high-throughput projects, contact us for a custom quote tailored to your specific requirements.

What is the difference between gene synthesis companies like Twist gene synthesis offerings from Twist Bioscience, GenScript, and Eurofins?

Major gene synthesis providers differ in their technology platforms, turnaround times, pricing models, and service breadth, whether you are evaluating Genewiz gene synthesis, a GenScript gene synthesis service, Twist, or other vendors. Twist Bioscience uses silicon-based DNA synthesis technology for high-throughput applications with products like Twist Gene Fragments, Twist plasmids, Twist plasmid synthesis solutions for complex constructs, and Twist Clonal Genes. GenScript offers comprehensive plasmid synthesis services with competitive pricing and established global presence, and our own platform similarly supports whole plasmid synthesis for complex constructs in the same way a GenScript plasmid synthesis workflow would. Eurofins Genomics leverages their oligonucleotide synthesis expertise with their GENEius optimization software, and many labs are familiar with Eurofins gene synthesis offerings when comparing providers. Our service combines the best aspects: AI-powered optimization for maximum expression, proprietary algorithms for complex sequence synthesis, transparent per-base pricing, flexible vector options, and comprehensive quality control. We offer standard and rush turnaround times comparable to industry leaders while maintaining 100% accuracy guarantee and providing personalized support for your specific research needs.

Can you synthesize complex genes with high GC content or repetitive sequences?

Yes, we specialize in synthesizing complex and challenging sequences that other providers may decline. Our proprietary synthesis technology and advanced algorithms can handle genes with extreme GC content (very high or very low), long homopolymeric runs, repetitive regions, strong secondary structures, and intricate hairpin formations. For complex sequences, we employ specialized assembly strategies, optimized reaction conditions, and additional quality control steps. While turnaround times may be extended for highly complex constructs, we provide realistic timelines during the quotation process and maintain our commitment to 100% sequence accuracy. Our sequence analysis tools automatically assess complexity during design and recommend optimization strategies when appropriate. Contact our technical team to discuss specific challenging sequences—we have successfully synthesized genes that other companies couldn't produce.

100% Accuracy
Sequence-verified
Every gene guaranteed

Fast Turnaround
From 5 business days
Rush options available

10+ Billion
Bases synthesized
Proven reliability

AI-Powered
Optimization technology
Maximum expression