Recombinant Antibody Production Services

From sequence to purified antibody in ~2 weeks (transient expression)

CHO stable-expression options available for larger-scale projects (~4–6 weeks). Transparent pricing and documented QC data you can trust.

Price starting from $600 / 1 mg. Bulk pricing from $500/antibody for multi-clone orders. Able to scale to gram level production.

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About recombinant antibodies

Recombinant antibodies are monoclonal antibodies produced in vitro from synthetic gene sequences. Compared to traditional antibody production methods like hybridoma, recombinantly produced antibodies are faster, more consistent, and scalable. They can achieve high purity and low endotoxin levels in controlled, animal-free environments, and can be adapted to GMP standards for therapeutic applications.

Service Highlights

High success rate

Decades of experience and optimized platform maximizes success across standard IgG and common formats.

Defined as delivering ≥1 mg purified antibody from provided VH/VL sequences (transient expression), excluding sequence errors.

Yield guarantee

From $600 (1 mg package). Bulk pricing from $500/antibody for multi-clone orders.

Multiple antibody formats

scFv, VHH, chimeric, bi/tri-specific, Fc-fusion, full length etc.

Easy scale up

High-throughput screening for 100+ clones, Gram level in 4 weeks.

Fast turnaround

From sequence to antibody in ~2 weeks (transient). Stable cell line: ~4–6 weeks.

US based support

Mitigate project risk by utilizing US-based project support.

Antibody engineering

Humanization, affinity purification, Isotype and framework switching.

Antibody validation

We offer protein production CRO and assay CRO service in WB, IHC, IF, ELISA, FACS, Biacore SPR, etc.

Production Scale Options

Scale from pilot to gram-level production with clear turnaround times and success-rate guarantees

Purified antibody (requested mg range)

QC docs: SDS-PAGE (>95%), endotoxin (<1 EU/mg)

Concentration by A280 (UV)

0.22 μm sterile filtration

PBS (preservative-free) or custom buffer

Pilot Scale

1-10 mg

Perfect for initial screening and functional validation. Small-scale expression lets you test multiple candidates quickly.

Turnaround: 2 weeks
Volume: 100 mL - 1 L

Standard Scale

10-100 mg

Most common for research applications, in vitro assays, and early-stage in vivo studies. Reliable yields with quality guarantees.

Turnaround: 3-4 weeks
Volume: 1-5 L

Large Scale

100 mg - 1+ gram

For extensive in vivo studies, therapeutic development, or large-scale production of monoclonal antibodies in commercial manufacturing. GMP-compatible processes available.

Turnaround: 4-6 weeks
Volume: 5-20+ L

High-throughput antibody production and screening options available for 100+ antibody candidates in parallel

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Facility images

Cell Line Construction Lab

Applikon Fermentor

Cell Incubators

Recombinant Monoclonal antibody production
Service Workflow & Timeline

Pricing note: Gene synthesis is priced separately at $0.20/bp.

Plasmid/vector construction, optional cell pool screening

Key output: Expression-ready vector / plasmid (and optional pool screening plan) | Timeline: ~1 week (transient) / ~2 weeks (stable)

We will synthesize the DNA sequence for the antibody's VH and VL variable regions and clone the gene into the expression vector. For transient expression, we will create an expression plasmid that's used to transfect the CHO cell line. For stable cell line expression, we will create a stable cell line (not included in the deliverables).
Recombinant expression

Key output: Expression harvest + in-process SDS-PAGE check | Timeline: ~1 week (transient) / ~2-3 weeks (stable)

We will express the antibody in a fermentor (large scale) or cell culture (small scale), similar to custom recombinant protein production workflows, until we harvest enough antibody fragments for purification. We will validate with SDS-PAGE at this stage and ensure the purity measured by SDS-PAGE is >95%. Additional purification service can be ordered.
Purification, characterization and Quality control

Key output: Purified antibody + standard QC results | Timeline: ~3-4 days

We will purify the antibody and characterize it for its affinity and titer. We will also ensure the endotoxin level is <1 EU/mg. Additional validation services can be ordered, as part of our CRO services, including Western blotting Service, ELISA service, IHC service, and Biacore SPR.
Delivery

Key output: Packaged & shipped antibody | Timeline: ~3-5 days prep + shipping time

We will prepare and ship the antibody for final delivery.

Timeline: 3-5 days internal prep; shipping time varies by location and method (typically 1-3 days domestic, 3-7 days international).

Total timeline: ~2 weeks for transient expression and ~4-6 weeks for stable cell line expression (excluding shipping).

High-Quality Purification & Documented QC

Every antibody undergoes rigorous purification and quality control testing. You receive comprehensive documentation that you can quickly interpret and trust, allowing you to proceed confidently.

Standard QC Testing Included:

Purity Analysis: SDS-PAGE (>95% purity guaranteed)
Endotoxin Testing: <1 EU/mg for all antibodies
Concentration: UV spectroscopy (A280)
Identity Confirmation: Mass spectrometry available
Sterility: 0.22 µm sterile filtration
Storage Buffer: PBS (preservative-free) or custom buffer

Optional Add-on Services:

Functional validation (ELISA, SPR, flow cytometry)
Aggregation analysis (DLS, analytical SEC)
SEC-HPLC for additional purity confirmation
Binding affinity determination (Biacore SPR)
Cell-based activity assays

Expression System Specifications

HEK293 Expression System

Transient transfection for rapid production
Human glycosylation patterns
Ideal for antibody fragments and complex formats
2-3 week turnaround from sequence

CHO Expression System

Industry-standard for therapeutic antibodies
Stable cell lines for consistent large-scale production
GMP-compatible processes
Superior for full-length IgG antibodies

All systems include:

Serum-free, chemically-defined media
Optimized transfection protocols
Expression enhancers for yield optimization
Scalable from mL to 20+ L

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Antibody Engineering Services

Engineer advanced constructs like bispecifics, fragments, and CAR components with expert support

Engineering Service	Description	Typical Applications
Chimeric Antibody Production	Graft your variable domains onto human constant regions to reduce immunogenicity before full humanization	In vivo studies, preclinical development, therapeutic candidates
Bispecific Antibodies	Engineer antibodies targeting two different antigens or epitopes simultaneously using validated formats	Cancer immunotherapy, dual-targeting therapeutics, research tools
Antibody Fragments	Generate scFv, Fab, or VHH formats for enhanced tissue penetration and specialized applications	Diagnostics, imaging, intracellular targets, blood-brain barrier penetration
Fc Engineering	Modify Fc region to enhance ADCC, eliminate effector functions, or extend half-life	Therapeutic optimization, in vivo model matching, side-effect reduction
Isotype & Species Switching	Convert between IgG subclasses or species to match your experimental model	Mouse to human conversion, subclass optimization, model compatibility
CAR Components	Design and produce single-chain variable fragments optimized for CAR-T construct assembly	Cell therapy development, CAR-T research, immunotherapy

Discuss Engineering Options

Sample Report

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FAQs

Frequently asked questions for recombinant antibodies production service.

Q1. What is recombinant antibody production?

Recombinant antibody production services are the process of generating antibodies in a laboratory setting using genetic engineering techniques. It involves cloning the genes encoding the antibody's variable regions into an expression vector and then performing monoclonal antibody synthesis in cells using suitable host systems.

Q2. What are the advantages of using recombinant antibodies?

Recombinant antibodies offer several advantages, including consistent quality, scalability, and the ability to modify antibody properties for specific applications. They also minimize the need for animal-based antibody generation.

Q3. Which host cells are used for recombinant antibody production?

Common host cells include mammalian cells (e.g., CHO, HEK293), bacterial cells (e.g., E. coli), and yeast cells (e.g., Pichia pastoris). Each has specific advantages based on the intended use.

Q4. Can you produce custom antibodies with specific characteristics?

Yes, we offer custom antibody production services. We can engineer antibodies with desired properties, such as humanization, affinity maturation, or incorporation of specific tags or modifications.

Q5. What is the typical timeline for recombinant antibody production?

The timeline varies depending on the complexity and scale of the project. In general, transient expression can yield antibodies within ~2 weeks, while stable cell line generation may take ~4 to 6 weeks.

Q6. What is the pricing structure for recombinant antibody production?

Our standard pricing starts from $600 for a 1 mg transient expression package. For multi-clone orders, bulk pricing is available starting from $500 per antibody. Gene synthesis is calculated separately at $0.20 per base pair. Contact us for a detailed quote on custom antibody production cost based on your specific requirements.

Q7. What formats of antibodies can you produce?

We can produce various antibody formats, including full-length IgG (all subclasses), scFv, Fab, VHH (nanobodies), chimeric antibodies, bispecific antibodies, and Fc-fusion proteins. Custom formats can also be developed upon request.

Q8. What quality control measures are in place?

All antibodies undergo comprehensive QC testing including SDS-PAGE for purity (>95%), endotoxin testing (<1 EU/mg), UV spectroscopy for concentration, and sterile filtration. Additional testing such as SEC-HPLC, mass spectrometry, and functional validation are available upon request.

Why Choose Boster Bio

Proven track record and state-of-the-art facilities

High Success Rate
Across standard formats

Fast Turnaround
~2 Weeks (transient)

Low Endotoxin
<1 EU/mg

Antibody Formats
Multiple