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Product Info Summary
|Size:||1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.|
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STAT1 Luciferase Reporter-HeLa Cell Line
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
The STAT1 Luciferase Reporter cell line is a stably transfected HeLa cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the interferon (IFN) gamma activation sequence-based STAT1 response element, so that the cell line is designed to measure the transcriptional activity of STAT1. As a transcription factor, Signal Transducer and Activator of Transcription 1 (STAT1) is activated through phosphorylation at tyrosine 701 in response to various cytokines and growth factors such as IFN-alpha, IFN-gamma, IL-6, EGF and PDGF. The phosphorylated STAT1 forms homodimers or heterodimers with STAT3, and the dimers translocate to nucleus in which DNA binding/promoter induction occurs. The STAT1 induction by IFN-gamma is shown in Figure 1.
Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Storage & Handling
Immediately upon receipt, store in liquid nitrogen.
Cite This Product
STAT1 Luciferase Reporter-HeLa Cell Line (Boster Biological Technology, Pleasanton CA, USA, Catalog # RC1012)
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Application:Monitor the STAT1 signaling pathway activity. Screen for activators or inhibitors of the STAT1 signaling pathway.
Culture conditions:Cells should be grown at 37°C with 5% CO2 using DMEM medium supplemented with 10% FBS and 1% Pen/Strep, plus 3 µg/ml of Puromycin. It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a 37°C water-bath, transfer to a tube containing 10 ml of growth medium without Puromycin, spin down cells, resuspend cells in pre-warmed growth medium without Puromycin, transfer resuspended cells to T25 flask and culture in 37°C-CO2 incubator. Leave the T25 flask in the incubator for 1~3 days without disturbing or changing the medium until cells completely recover viability and become adherent. Once cells are over 90% adherent, remove growth medium and passage the cells through trypsinization and centrifugation. At first passage, switch to growth medium containing Puromycin. Cells should be split before they reach complete confluence. To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly.
Functional validation:A. Response of STAT1 HeLa cells to IFN-gamma.1. Harvest STAT1 HeLa cells and seed cells into a white solid-bottom 96-well microplate in 100 µl of growth medium at 5 x 10^4 cells/well. 2. Incubate cells at 37°C in a CO2 incubator for overnight. 3. The next day, stimulate cells with different concentrations of human IFN-gamma. 4. Incubate at 37°C in a CO2 incubator for 6-16 hours. 5. Add 50 µl of luciferase assay reagent per well. 6. Incubate at room temperature for 1-5 minutes and measure luminescence using a microplate luminometer.
Validation Images & Assay Conditions
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Fig-1: Induction of STAT1 activity by IFN-gamma in STAT1 HeLa cells.
Gene/Protein Information For STAT1 (Source: Uniprot.Org, NCBI)
Signal transducer and activator of transcription 1-alpha/beta
transcription factor STAT family
CANDF7; DKFZp686B04100; ISGF-3; ISGF-3,91kD; signal transducer and activator of transcription 1, 91kDa; signal transducer and activator of transcription-1; STAT1; STAT91 STAT1 CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91 signal transducer and activator of transcription 1 signal transducer and activator of transcription 1-alpha/beta|signal transducer and activator of transcription 1, 91kD|signal transducer and activator of transcription 1, 91kDa|transcription factor ISGF-3 components p91/p84*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
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