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This antibody is highly specific and efficient, suitable for Western blot detection of Anti-PI3K p85 alpha Antibody protein in mouse hippocampus, with only minor nonspecific bands observed.

Excellent, submitted by on
SKU A00318-1
Application Western Blot
Sample Mouse brain tissue
Sample Processing Description Mouse brain tissue was lysed in RIPA buffer supplemented with protease inhibitors at 4°C for 2 hours. The lysate was centrifuged to collect the supernatant, and protein concentration was determined. After adjusting the concentration, samples were mixed with 5× protein loading buffer and heated at 95–100°C for 10 minutes to denature. Then, 15 μL of each protein sample was loaded per lane for electrophoresis.
Other Reagents Blocking buffer
Primary Antibody PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband®
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows a schematic representation of Western blot results for PIK3R1 and the loading control β-actin in mouse brain tissues from normal mice, model group, and mice treated with high and low doses of drug AB. Although the expression differences between experimental groups are not pronounced, the Western blot results obtained with this antibody are still clear and well-defined.

The antibody has good specificity, with a clear target band at the correct position and minimal non-specific bands.

Excellent, submitted by on
SKU M00566
Application Western Blot
Sample Human A549 cells, HCC1833 cells, LU65 cells, PC-9 cells
Sample Processing Description After normal cell culture, total proteins were extracted using RIPA lysis buffer with protease inhibitors. Protein concentration was determined by BCA assay, and then the four samples were adjusted to a uniform concentration of 3 mg/mL using loading buffer.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody c-Fos Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The purpose of the experiment is to examine the differences in ADAM10 protein expression among different types of human lung cancer cells, in order to select suitable cells for subsequent experiments.

The antibody shows a clear target band at the correct position, and the results are good.

Excellent, submitted by on
SKU M00297
Application Western Blot
Sample Mouse hippocampal tissue
Sample Processing Description An Alzheimer’s disease (AD) model was induced by intracerebroventricular injection of streptozotocin (STZ) in mice. The mice were subsequently treated with four different doses of Qianlenta Heji, and total protein was extracted from the hippocampal tissues.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody c-Fos Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary c-FOS serves as a marker of neuronal activation under pathological conditions. Its expression is low in normal brain tissue but elevated in AD model mice, and is reduced after treatment. In the WB assay, lane 1 corresponds to hippocampal tissue from normal mice, lane 2 to AD model mice, lanes 3–5 to hippocampi treated with increasing doses of Qianlenta Heji, and lane 6 to a positive control drug. The results demonstrate that Qianlenta Heji exhibits therapeutic effects in the AD model.

The antibody has high specificity and strong potency, producing excellent results.

Excellent, submitted by on
SKU A00220-4
Application Immunofluorescence
Sample Normal and preterm human placenta
Sample Processing Description Clinically collected term and preterm placentas were processed for paraffin embedding and sectioning in the longitudinal orientation, allowing visualization of both the amnion and chorion.
Other Reagents Goat serum, DAPI (AR1176,Boster Bio),Anti-fade mounting medium
Primary Antibody Cd86 Antibody Picoband®
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody DyLight 594 Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:500, 1 hour in room temperature
Detection Imaging system:Leica DM2500
Results Summary CD86 is a marker of M1 macrophages. In normal placenta, macrophages are generally absent or very few, and M1 macrophages are even less. In preterm placenta, due to inflammation, the number of macrophages increases and they tend to polarize toward the M1 type. Our experimental results strongly support this theory and are consistent with the expected data.

The antibody shows a clear target band at the correct position, with minimal background, and the results are excellent.

Excellent, submitted by on
SKU A03449-3
Application Western Blot
Sample Human keratinocytes isolated from skin
Sample Processing Description Human Caco-2 cell line
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody ME1 Antibody Picoband®
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The protein level of ME1 in LPS-stimulated CACO-2 cells is higher than that in normal CACO-2 cells.

The target band of this antibody is clear and at the correct position, and it performs better than antibodies from other brands.

Excellent, submitted by on
SKU M01245
Application Western Blot
Sample Human keratinocytes isolated from skin
Sample Processing Description Keratinocytes isolated from normal skin tissue.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody Albumin Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary Human serum albumin (ALB) is the most abundant protein in plasma, with key functions including maintaining colloid osmotic pressure, transporting substances, providing antioxidant activity, and regulating inflammation. Clinically, ALB is widely used to treat hypoalbuminemia, shock, burns, and other conditions. ALB was long thought to be secreted exclusively by hepatocytes, but recent studies suggest that other cell types may also be capable of synthesizing it. The purpose of this experiment was to verify whether human skin keratinocytes can synthesize ALB. The results show that skin keratinocytes are indeed capable of producing ALB.

The target band of this antibody is clear and at the correct position, and it performs better than antibodies from other brands.

Excellent, submitted by on
SKU A00339-2
Application Western Blot
Sample Human keratinocytes isolated from skin
Sample Processing Description Keratinocytes isolated from normal skin tissue.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody Cytochrome P450 3A4/CYP3A4 Antibody
Primary Incubation 1:5000, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary CYP3A4 is a major enzyme responsible for drug metabolism in the body. More than 50% of commonly used drugs in humans are metabolized and cleared through CYP3A4. It catalyzes various chemical reactions, such as oxidation and reduction, converting drugs into more water-soluble forms that are easier to excrete. This experiment aimed to verify whether human skin keratinocytes synthesize CYP3A4 protein and to estimate its approximate level. The results show that skin keratinocytes do synthesize CYP3A4 protein, and its expression level is considerable.

The target band of this antibody is clear and at the correct position, with minimal nonspecific bands, and the results are good.

Excellent, submitted by on
SKU M00207-1
Application Western Blot
Sample huaman 293 cells
Sample Processing Description RHOB detection was performed using total protein extracted from normal 293 cells and 293 cells with RHOB overexpression.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody ATF4 Rabbit Monoclonal Antibody
Primary Incubation 1:2500, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary We generated a stable RHOB overexpression cell line using 293 cells. The results show that the RHOB expression level in the transfected samples is significantly higher than in the normal 293 cells, indicating successful transfection.

The antibody shows a clear target band at the correct position and has strong affinity for phosphorylated IRE1 protein from pig.

Excellent, submitted by on
SKU M00371
Application Western Blot
Sample Porcine intestinal tissue
Sample Processing Description Intestinal tissues from different segments of pigs infected with swine fever.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody ATF4 Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary ATF4 is a key regulator of the integrated stress response. When cells encounter conditions such as oxidative stress or endoplasmic reticulum stress, the expression of ATF4 is rapidly induced and upregulated. The aim of this experiment is to determine the expression levels of ATF4 protein in different intestinal segments infected with classical swine fever.

The antibody shows a clear target band at the correct molecular weight and exhibits strong affinity for phosphorylated EIF2A protein in pig samples.

Excellent, submitted by on
SKU P04387
Application Western Blot
Sample Porcine intestinal tissue
Sample Processing Description Intestinal tissues from different segments of pigs infected with swine fever.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody Anti-Phospho-EIF2S1 (S51) Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary Phosphorylation of eIF2α is one of the most important switches controlling intracellular protein synthesis. When cells encounter conditions such as oxidative stress or endoplasmic reticulum stress, phosphorylated eIF2α rapidly suppresses the majority of protein synthesis to help the cell cope with the crisis. The purpose of this experiment is to determine the expression levels of phosphorylated EIF2A protein in different intestinal segments infected with classical swine fever virus.