
| SKU | PA2290 |
|---|---|
| Application | Western Blot |
| Sample | Protein extracts from LA795 and 16HBE cells. |
| Sample Processing Description | After collecting the cells, lyse them with RIPA buffer containing protease inhibitors, quantify the protein using the BCA assay, and add loading buffer proportionally. Denature by heating at 98 °C. Load the samples onto SDS-PAGE, with 30 μg of total protein per lane. |
| Primary Antibody | Anti-TMEM16A/ANO1 Antibody Picoband® |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Blocking Agent | 5% non-fat milk |
| Secondary Antibody | HRP-conjugated goat anti-rabbit IgG |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Detection | Substrate: ECL substrate, Imaging system: Tanon |
| Results Summary | The TMEM16A antibody we previously used was never ideal; despite long-term optimization, it was difficult to obtain clear bands. This time, by using BOSTER’s polyclonal antibody, we achieved much better experimental results. The bands were successfully exposed in essentially one attempt, with clear signals and no non-specific bands. We are very satisfied with Boster’s antibody. |










