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Anti-BDNF (PB9075) shows clear, specific bands in mouse brain tissues by WB, with BDNF levels markedly reduced in depressed mice and restored after treatment, demonstrating excellent antibody performance.

Excellent, submitted by on
PB9075 wb
SKU PB9075
Application Western Blot
Sample mouse brain tissue
Sample Processing Description ① Hippocampal tissue from normal mice, ② Hippocampal tissue from depressed mice, ③ Hippocampal tissue from depressed mice treated with our in-house drug, ④ Hippocampal tissue from depressed mice treated with a positive control drug. Total protein was extracted from all samples.
Other Reagents RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody BDNF Antibody Picoband®
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:10000, 1 h in RT
Detection Substrate: ECL substrate, Image system: ChemiDoc MP
Results Summary BDNF belongs to the neurotrophin family and functions by binding to the TrkB receptor on the surface of neurons, activating intracellular signaling pathways to maintain, protect, and enhance neuronal function. In simple terms, it helps brain cells stay healthier, more active, and better connected. Experimental results show that BDNF levels are significantly decreased in the brains of depressed mice, but are restored after treatment.

The PI3K p85 alpha Antibody (M00318-2) showed clear and specific bands in WB of rat skeletal muscle, with PI3K p85 expression increased in the model group and decreased in a dose-dependent manner in the treatment groups, consistent with expectations.

Excellent, submitted by on
M00318-2 wb
SKU M00318-2
Application Western Blot
Sample Rat skeletal muscle tissue
Sample Processing Description Normal rat skeletal muscle, injury model, and high-, medium-, and low-dose treatment groups, with total protein extracted.
Other Reagents RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer,Blocking buffer
Primary Antibody PI3 Kinase p85 alpha Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:10000, 1 h in RT
Detection Substrate: ECL substrate, Image system: ChemiDoc MP
Results Summary Based on the experimental results, the band of PI3K p85 is clear. The PI3K p85 level in the model group is significantly higher than in the normal group, whereas in the treatment group, the PI3K p85 level decreases with increasing drug dose, which is consistent with expectations.

The AKT1 Antibody (M00024-1) showed clear, specific bands in WB of rat skeletal muscle, with expression changes consistent with expectations.

Excellent, submitted by on
SKU M00024-1
Application Western Blot
Sample Rat skeletal muscle tissue
Sample Processing Description Normal rat skeletal muscle, injury model, and high-, medium-, and low-dose treatment groups, with total protein extracted.
Other Reagents RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody AKT1 Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:10000, 1 h in RT
Detection Substrate: ECL substrate, Image system: ChemiDoc MP
Results Summary The AKT1 bands are clear and correctly positioned; expression is significantly higher in the model group than in the control, and decreases with increasing drug dose in the treatment groups, consistent with expectations.

MTOR Antibody (M00003) WB on rat skeletal muscle shows clear bands, with higher MTOR in the model and dose-dependent reduction in treatment groups.

Excellent, submitted by on
SKU M00003
Application Western Blot
Sample Rat skeletal muscle tissue
Sample Processing Description Total protein was extracted from rat skeletal muscle tissue: ① normal, ② injury model, ③ high-dose drug treatment, ④ medium-dose drug treatment, ⑤ low-dose drug treatment.
Other ReagentsRIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody mTOR/Tor Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:10000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary The results show clear MTOR bands, with higher levels in the injury model compared to normal, and a dose-dependent decrease in the treatment groups, as expected.

This Western blot experiment using COL3A1 antibody (M00788-1) on mouse cardiac tissues from 3-, 6-, 12-, and 18-month-old mice shows that COL3A1 levels are generally similar across ages, with a slight but not significant increase as age progresses.

Excellent, submitted by on
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SKU M00788-1
Application Western Blot
Sample mouse cardiac tissue
Sample Processing Description Total protein was extracted from mouse cardiac tissues at 3, 6, 12, and 18 months of age.
Other Reagents RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody Collagen III Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:10000, 1 h at RT
Detection Substrate: ECL substrate, Image system: ChemiDoc MP
Results Summary This experiment aimed to investigate changes in COL3A1 expression in mouse cardiac tissues from 3-, 6-, 12-, and 18-month-old mice (representing young, middle-aged, middle-old, and old stages), and the results show that COL3A1 levels are generally similar across ages, with a slight but not significant increase as age progresses.
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WB of COL1A1 (Cat# PA2140-1) in mouse cardiac tissues from 3-, 6-, 12-, and 18-month-old mice shows that COL1A1 levels clearly increase with age.

Excellent, submitted by on
SKU PA2140-1
Application Western Blot
Sample mouse cardiac tissue
Sample Processing Description Total protein was extracted from mouse cardiac tissues at 3, 6, 12, and 18 months of age.
Other Reagents RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody Collagen I/COL1A1 Antibody Picoband®
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:10000, 1 h in RT
Detection Substrate: ECL substrate, Image system: ChemiDoc MP
Results Summary This experiment aimed to investigate changes in COL1A1 expression in mouse cardiac tissues from 3-, 6-, 12-, and 18-month-old mice (representing young, middle-aged, middle-old, and old stages), and the results show that COL1A1 levels clearly increase with age.

The Anti-α-SMA antibody (Cat# BM3902) shows clear and specific bands with low background in WB analysis of mouse heart tissues across different ages, with results consistent with expected trends.

Excellent, submitted by on
SKU M01072-1
Application Western Blot
Sample mouse cardiac tissue
Sample Processing Description Mouse cardiac tissues were collected from 3-, 6-, 12-, and 18-month-old mice, and total protein was extracted.
Other Reagents RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:10000, 1 h at RT
Detection Substrate: ECL substrate
Imaging system: ChemiDoc MP
Results Summary This experiment aimed to investigate the changes in α-SMA expression in mouse cardiac tissues from 3-, 6-, 12-, and 18-month-old mice (representing young, middle-aged, and aged stages). The results show that α-SMA levels are generally similar across different ages, with a slight but not significant increase as age progresses.

The Anti-CD31 antibody (Cat# A01513-2) shows high specificity in mouse lung IF, clearly labeling endothelial cells with accurate localization.

Excellent, submitted by on
SKU A01513-2
Application Immunofluorescence
Sample mouse lung tissue
Sample Processing Description Left lung from normal mouse was washed 3 times with PBS to remove blood cells, fixed in formaldehyde for 24 h, and paraffin-embedded for sectioning.
Other ReagentsGoat serum, DAPI (Cat# AR1176, Boster Bio)
Primary Antibody CD31/Pecam1 Antibody Picoband®
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro594 Conjugated (Cat# BA1142, Boster Bio)
Secondary Incubation 1:500, 45min in 37℃
Detection Image system:Leica DM2500
Results Summary CD31, also known as PECAM-1, is a key marker of vascular endothelial cells and plays a central role in biomedical research, especially in angiogenesis and tumor microenvironment studies. In this experiment, CD31 IF was performed to label endothelial cells, facilitating subsequent evaluation of vascular density distribution. The results show clear endothelial staining with precise localization, yielding excellent outcomes.

Western blot analysis using Anti-COL3A1 Antibody (M00788-1) showed clear and specific bands in mouse myocardium samples from 3-, 6-, 12-, and 18-month-old mice, with overall comparable COL3A1 expression and a slight, non-significant increase with age.

Excellent, submitted by on
SKU M00788-1
Application Western Blot
Sample mouse brain tissue
Sample Processing Description Myocardial tissues were collected from mice at 3, 6, 12, and 18 months of age, and total protein was extracted.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, blocking buffer
Primary Antibody Collagen III Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:10000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary This experiment aimed to study the changes in COL3A1 protein in mouse myocardium at 3, 6, 12, and 18 months of age (representing young, adult, middle-aged, and old mice). The results show that COL3A1 levels are largely similar across ages, with a slight, non-significant increase as age progresses.

Western blot using Anti-ER/ESR1 Antibody (M00057-2) in mouse brain tissues showed clear, specific ESR1 bands across normal, disease model, and AB drug-treated groups, with β‑actin as a consistent loading control.

Excellent, submitted by on
SKU M00057-2
Application Western Blot
Sample mouse brain tissue
Sample Processing Description Mouse brain tissues were lysed in RIPA buffer containing a protease inhibitor cocktail at 4°C for 2 hours, centrifuged to collect the supernatant, and protein concentration was determined. After adjusting the concentration, samples were mixed with 5× protein loading buffer, denatured by heating at 95–100°C for 10 minutes, and 15 μL of protein was loaded per lane for SDS-PAGE.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody goat anti rabbit secondary antibodies
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary This antibody is highly sensitive, produces clear WB bands, is reusable, offers excellent cost-effectiveness, and demonstrates a clear advantage over similar international products, making it highly recommended for use.