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In this WB experiment using Anti-CAMP antibody (Cat# A05475-1) on MADB106 rat mammary carcinoma cells, CAMP protein secretion was markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor.

Excellent, submitted by on
SKU A05475-1
Application Western blot
Sample MADB106 rat mammary carcinoma cells
Sample Processing Description MADB106 cells under normal culture conditions , MADB106 cells treated with agonist , MADB106 cells treated with inhibitor
Other ReagentsRIPA Lysis Buffer, Protease Inhibitor, Resolving Gel Solution ,Transfer Buffer ,Blocking Buffer
Primary Antibody Camp Antibody Picoband®
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody 1:10,000, HRP-conjugated Goat Anti-Rabbit IgG
Secondary Incubation 1h at 37℃
Detection Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary CAMP, also known as cathelicidin antimicrobial peptide or LL-37, is an important antimicrobial peptide with dual functions: directly killing pathogens and modulating immune responses. It plays a complex role in maintaining the homeostasis of barrier tissues such as skin and in influencing tumor progression. In breast cancer, CAMP may promote tumor development by enhancing angiogenesis and cancer cell proliferation. In this experiment, CAMP secretion was markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor.

In this Western blot (WB) experiment using Anti-AQP5 antibody (Cat# A03085) on MADB106 rat mammary carcinoma cells, AQP5 protein levels were significantly increased with agonist treatment and markedly reduced with inhibitor treatment.

Excellent, submitted by on
SKU A03085
Application Western blot
Sample MADB106 rat mammary carcinoma cells
Sample Processing Description MADB106 cells under normal culture conditions , MADB106 cells treated with agonist , MADB106 cells treated with inhibitor
Other ReagentsRIPA Lysis Buffer, Protease Inhibitor, Resolving Gel Solution ,Transfer Buffer ,Blocking Buffer
Primary Antibody Aquaporin 5/AQP5 Antibody Picoband®
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody 1:10,000, HRP-conjugated Goat Anti-Rabbit IgG
Secondary Incubation 1h at 37℃
Detection Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary AQP5 (Aquaporin 5) is a transmembrane water channel protein that primarily regulates cellular water transport and plays a critical and complex role in tumor progression. In breast cancer, high AQP5 expression is often associated with increased tumor invasiveness, lymph node metastasis, and poor patient prognosis. In this experiment, AQP5 protein levels were markedly increased in cells treated with the agonist, whereas they were significantly reduced in cells treated with the inhibitor.

The Anti-TRPV1 antibody (A00128-4) showed clear and correctly positioned bands in WB of MADB106 rat breast cancer cells, with increased TRPV1 expression upon agonist treatment and decreased expression upon inhibitor treatment.

Excellent, submitted by on
SKU A00128-4
Application Western blot
Sample Rat MADB106 breast cancer cells
Sample Processing Description Normally cultured MADB106 cells, MADB106 cells treated with an agonist, MADB106 cells treated with an inhibitor
Other ReagentsRIPA lysis buffer (Cat. No. AR0102, Bosterbio, Wuhan) Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody TRPV1 Antibody Picoband®
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody 1:10,000, HRP-conjugated Goat Anti-Rabbit IgG
Secondary Incubation 1h at 37℃
Detection Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary The role of TRPV1 in breast cancer research is highly complex, varying with cell type, tumor subtype, and tumor microenvironment, and in some cases even showing contradictory effects. The current consensus is that TRPV1 is not only a potential therapeutic target for breast cancer but also an important prognostic and regulatory factor. Experimental results show that TRPV1 protein expression markedly increases in cells treated with an agonist, while it decreases in cells treated with an inhibitor.

The Anti-c-FOS antibody (M00297) showed clear and specific immunofluorescence staining in mouse brain, with markedly increased c-Fos expression in neurons of the acute ischemic region compared to normal brain.

Excellent, submitted by on
SKU M00297
Application Immunocytochemistry
Sample mouse brain
Sample Processing Description Normal brain tissue and brain tissue from a cerebral infarction model
Other ReagentsGoat serum, DAB Chromogenic Solution, Anti-fade mounting medium
Primary Antibody c-Fos Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody DyLight 594 Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 45 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary c-Fos is a marker of activated neural cells, especially neurons. During the acute phase of cerebral infarction, as a strong stress and injury stimulus, it drives c-Fos expression in the brain—particularly in the peri-infarct region—far higher than in normal resting brain. The experimental results show clear staining of positive cells, and the expression levels are consistent with expectations.

The Anti-FAP antibody (M00422) showed clear and specific IHC staining in human hepatocellular carcinoma, with low expression in adjacent normal tissue and strong expression in tumor stroma.

Excellent, submitted by on
SKU M00422
Application Immunohistochemistry
Sample human liver cancer
Sample Processing Description Collected clinical surgical human hepatocellular carcinoma and adjacent peritumoral tissues were fixed in formalin and embedded in paraffin.
Other ReagentsGoat serum, DAB Chromogenic Solution
Primary Antibody FAP1 Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Two-step IHC kit (Immunohistochemistry kit
Secondary Incubation 30 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary FAP (fibroblast activation protein) expression is mainly associated with stromal cells in the tumor microenvironment and is very low in normal human tissues. Its role in hepatocellular carcinoma (HCC) is complex, but overall it functions as an important pro-tumor factor. High FAP expression is associated with poor prognosis. It can remodel the extracellular matrix, promote tumor invasion and metastasis, and suppress anti-tumor immunity. In this study, FAP showed low expression in peritumoral tissues (close to normal tissue) and high expression in the tumor stroma of HCC.

The Anti-TH antibody (M01917) demonstrated excellent specificity and clear fluorescence staining in IF analysis of paraffin-embedded mouse striatum sections, accurately labeling dopaminergic neurons with minimal background.

Excellent, submitted by on
SKU M01917
Application Immunofluorescence
Sample mouse brain
Sample Processing Description Sagittal sections of mouse striatum were fixed in formaldehyde for 48 hours and paraffin-embedded.
Other ReagentsGoat serum，DAPI，Anti-fade mounting medium
Primary Antibody Iba1 Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody 1:500, Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro594 Conjugated
Secondary Incubation 45 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary TH is a marker of catecholaminergic neurons and can specifically label and identify dopaminergic, noradrenergic, and adrenergic neurons. In this experiment, TH immunofluorescence was performed to observe the distribution of dopaminergic neurons in the striatum. The results show clear staining and precise localization.

In IHC of human skin cancer sections, the CEACAM5 antibody (A00356) clearly distinguished melanoma from other skin cancers, showing specific staining with low background.

Excellent, submitted by on
SKU M12477-15
Application Immunohistochemistry
Sample normal and preterm human placentas
Sample Processing Description Clinically collected normal and preterm placentas were sectioned longitudinally (with amnion and chorion visible) and prepared as paraffin-embedded sections.
Other ReagentsTris-EDTA Antigen Retrieval Solution, DAB Chromogenic Solution
Primary Antibody Histone H3 (acetyl K27) Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Polymer anti-rabbit IgG-HRP IHC detection kit
Secondary Incubation 45 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary Histone H3 (acetyl K27) is the acetylated form of histone H3 at lysine 27 and serves as a sensitive indicator of epigenetic abnormalities in placental cells; IHC results showed higher expression in preterm placentas than in normal placentas.

In an immunofluorescence experiment using normally cultured Caco-2 cells, the ZO-1 antibody (PB9234) showed clear and specific membrane staining with low background, demonstrating reliable performance.

Excellent, submitted by on
SKU PB9234
Application Immunofluorescence
Sample human Caco-2 cell line
Sample Processing Description Cells were normally cultured in 24-well plates using MEM supplemented with 20% fetal bovine serum. When the cell density reached approximately 60%, the culture was terminated. The medium was removed, cells were washed three times with PBS, fixed with 4% paraformaldehyde for 15 minutes, and then washed three times with PBS before further use.
Other ReagentsGoat serum, DAPI, and an anti-fade mounting medium.
Primary Antibody ZO1 tight junction protein/TJP1 Antibody Picoband®
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro488 Conjugated
Secondary Incubation 45 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary Caco-2 cells are a “gold standard” in vitro model for studying intestinal epithelial function, and ZO-1 is a key marker of barrier integrity. The images show immunofluorescence staining of ZO-1 in normally cultured Caco-2 cells to evaluate antibody performance. Clear membrane localization and accurate staining indicate that this antibody is suitable for downstream research applications.

Using IHC on paraffin-embedded human placental tissues, the Anti-Histone H3 (acetyl K27) antibody (M12477-15) showed high specificity and clear staining, with higher H3K27 acetylation in preterm placentas than in normal controls.

Excellent, submitted by on
SKU M12477-15
Application Immunohistochemistry
Sample normal and preterm human placentas
Sample Processing Description Clinically collected normal and preterm placentas were sectioned longitudinally (with amnion and chorion visible) and prepared as paraffin-embedded sections.
Other ReagentsTris-EDTA Antigen Retrieval Solution, DAB Chromogenic Solution
Primary Antibody Histone H3 (acetyl K27) Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Polymer anti-rabbit IgG-HRP IHC detection kit
Secondary Incubation 45 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary Histone H3 (acetyl K27) is the acetylated form of histone H3 at lysine 27 and serves as a sensitive indicator of epigenetic abnormalities in placental cells; IHC results showed higher expression in preterm placentas than in normal placentas.

IF using Anti-CD31 antibody (A01513-2) showed specific, strong, and stable endothelial staining, revealing expanded and inflamed microvessels in treated mouse skin compared to controls.

Excellent, submitted by on
SKU A01513-2
Application Immunofluorescence
Sample Paraffin-embedded mouse skin sections
Sample Processing Description Mouse skin samples were divided into three groups: (i) normal control, (ii) congestion group, treated with ethanol, and (iii) inflammation group, stimulated with LPS.
Other Reagents Goat serum, DAPI, Anti-fade mounting medium
Primary Antibody CD31/Pecam1 Antibody Picoband®
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody DyLight 488–conjugated Goat Anti-Rabbit IgG (H+L)) (Boster, BA1127)
Secondary Incubation 1:500, 45 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary IF staining with Boster CD31 antibody produced high-quality vascular images. In the control group, vessels were small and well-defined. In the congestion group (ethanol-treated), CD31 staining revealed markedly dilated vascular lumens. In the inflammation group (LPS-treated), vessel dilation was accompanied by changes in tissue spacing. Combined with Prussian blue staining and magnetic signal measurements, we observed that despite extreme vessel dilation in the congestion group, magnetic signals were not significantly elevated; only the inflammation group, with extensive macrophage infiltration and uptake, showed high magnetic signals.