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IF using Anti-CD31 antibody (A01513-2) showed specific, strong, and stable endothelial staining, revealing expanded and inflamed microvessels in treated mouse skin compared to controls.

Excellent, submitted by on
SKU A01513-2
Application Immunofluorescence
Sample Paraffin-embedded mouse skin sections
Sample Processing Description Mouse skin samples were divided into three groups: (i) normal control, (ii) congestion group, treated with ethanol, and (iii) inflammation group, stimulated with LPS.
Other Reagents Goat serum, DAPI, Anti-fade mounting medium
Primary Antibody CD31/Pecam1 Antibody Picoband®
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody DyLight 488–conjugated Goat Anti-Rabbit IgG (H+L)) (Boster, BA1127)
Secondary Incubation 1:500, 45 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary IF staining with Boster CD31 antibody produced high-quality vascular images. In the control group, vessels were small and well-defined. In the congestion group (ethanol-treated), CD31 staining revealed markedly dilated vascular lumens. In the inflammation group (LPS-treated), vessel dilation was accompanied by changes in tissue spacing. Combined with Prussian blue staining and magnetic signal measurements, we observed that despite extreme vessel dilation in the congestion group, magnetic signals were not significantly elevated; only the inflammation group, with extensive macrophage infiltration and uptake, showed high magnetic signals.

IHC using Tyrosine Hydroxylase/TH Antibody Picoband® (P00683) showed clear staining with no background, revealing markedly reduced TH expression in the medulla of Alzheimer’s mouse brain compared to normal mouse brain.

Excellent, submitted by on
SKU PB9449
Application Immunohistochemistry
Sample Normal mouse brain and Alzheimer’s model mouse brain tissue
Sample Processing Description Paraffin-embedded normal mouse brain and Alzheimer’s model mouse brain.
Other Reagents Goat serum, DAB
Primary Antibody Tyrosine Hydroxylase/TH Antibody Picoband®
Primary Incubation 1:500, overnight at 4 ℃
Secondary Antibody Two-step IHC kit
Secondary Incubation 37 minutes in 37 ℃
Detection Imaging system:Leica DM2500
Results Summary TH is a marker of catecholaminergic neurons, specifically labeling dopaminergic, noradrenergic, and adrenergic neurons. IHC results showed that TH expression in the medulla was markedly lower in Alzheimer’s mouse compared to normal mouse.

IHC with HDAC5 Antibody Picoband® (A01230-6) showed clear staining and low background, with significantly higher HDAC5 levels in the cortex of Alzheimer’s mice, suggesting potential as a biomarker.

Excellent, submitted by on
SKU A01230-6
Application Immunohistochemistry
Sample Normal mouse brain and Alzheimer’s model mouse brain
Sample Processing Description Paraffin-embedded normal mouse brain and Alzheimer’s model mouse brain.
Other Reagents Goat serum, DAB chromogen
Primary Antibody Anti-HDAC5 Antibody Picoband®
Primary Incubation 1:400, overnight at 4 ℃
Secondary Antibody Two-step IHC kit
Secondary Incubation 30 minutes in 37 ℃
Detection Imaging system: Leica DM2500
Results Summary The results showed that HDAC5 levels in the cortex were significantly higher in Alzheimer’s mice than in normal mice, suggesting its potential as a biomarker for further validation.

Using Myeloperoxidase/MPO Antibody Picoband® (PA1054) in IHC, MPO showed low expression in normal mouse skin and was markedly increased in burned skin, with clear staining and expected results.

Excellent, submitted by on
SKU PA1054
Application Immunohistochemistry
Sample mouse skin tissue
Sample Processing Description Male BALB/c mice aged 6–8 weeks were used. (1) Normal dorsal skin was collected from untreated mice. (2) Burn injury was induced on the dorsal skin using a burn device. After one week of housing, hair was removed with depilatory cream. Skin samples were collected from normal mice (dorsal skin) and burned mice (burned area), fixed in formalin for 72 hours, and embedded in paraffin for sectioning./td>
Other ReagentsTris-EDTA Antigen Retrieval Buffer (50×, pH 9.0), DAB Chromogen Kit
Primary Antibody Myeloperoxidase/MPO Antibody Picoband®
Primary Incubation 1:100, overnight at 4 ℃
Secondary Antibody Polymer Anti-Rabbit IgG–HRP Immunohistochemistry Kit
Detection Imaging system:Leica DM2500
Results Summary MPO (myeloperoxidase) is a lysosomal enzyme mainly present in neutrophils and monocytes/macrophages. Its core function is to generate reactive oxygen species, and therefore it plays a “double-edged sword” role in innate immune defense and inflammation-related diseases. MPO is present at very low levels in normal skin, but in burned skin, a large number of macrophages accumulate around the injured tissue, accompanied by a significant increase in MPO expression. Based on the immunohistochemical results, the staining is clear and the findings are consistent with expectations.

Produced clear, sharp bands with no background. Excellent specificity and consistency across replicates. Ideal as a loading control for WB.

Excellent, submitted by on
SKU A01263
Application Western Blot
Sample Human 293T and A549 cell lysates, Mouse brain tissue
Sample Processing Description Cells were lysed in RIPA buffer containing protease inhibitors. Lysates were clarified by centrifugation and quantified using a BCA assay. 30–35 µg of protein was loaded per lane on a 5–20% SDS-PAGE gel and transferred to nitrocellulose membranes.
Primary Antibody Anti-beta Actin ACTB Antibody
Primary Incubation 1:1000, incubated overnight at 4°C.
Secondary Antibody Goat anti-rabbit IgG-HRP (Catalog # BA1054).
Secondary Incubation 1:5000 dilution, 1 hour at room temperature.
Other Reagents used 5% non-fat milk/TBST blocking buffer, ECL Plus substrate, Azure Biosystems c600 imaging system.
Detection Chemiluminescent detection. Strong single band observed at ~42 kDa, corresponding to beta actin.
Results Summary Produced clear, sharp bands with no background. Excellent specificity and consistency across replicates. Ideal as a loading control for WB.

This antibody is highly specific and efficient, suitable for Western blot detection of C1QBP protein in mouse liver and brown adipose tissue, with no nonspecific bands observed. However, due to experimental conditions and the particular characteristics of

Excellent, submitted by on
SKU M01439
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody Anti-GC1q R Rabbit Monoclonal Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows Western blot results of the target protein and the loading control in mouse liver and brown adipose tissue from normal and treated groups, with or without enzymatic digestion. The target bands in the liver are clear and well defined, and the experimental results are satisfactory.

This antibody is highly specific and efficient, suitable for Western blot detection of TTF2 protein in rat colon, with clear bands.

Excellent, submitted by on
SKU A07013-2
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody TFF2 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows Western blot results of the target protein TTF2 and the internal control Actin in rat colon from the normal control group, model group, low/medium/high-dose traditional Chinese medicine treatment groups, and the Western medicine treatment group. The target bands are clear and well defined, and the experimental results are satisfactory.

An shRNA targeting Atxn7 displayed a decrease in Atxn7 abondance showing specificity of the antibody.

Excellent, submitted by on
SKU DZ41648
Application Western Blot
Sample 3T3 Cell lysate
Sample Processing Description Nuclear and Cytoplasmic Extraction was performed on 3T3 cells with NE-PER kit by following the protocol and by adding protease and phosphatase inhibitors. Total protein was quantified with Qubit Protein Assay. Lysates were mixed with 4× Laemmli Sample Buffer and 2-mercaptoethanol, and then heated at 95°C for 5 min.
Primary Antibody Anti-Mouse Atxn7 Antibody
Primary Incubation Incubated in non-fat dry milk in TBST overnight at 4°C with antibody dilution at 1:1000 dilution
Secondary Antibody Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
Secondary Incubation 1:4000 dilution in milk for 1 hour at room temperature
Other Reagents used SuperSignal West Pico PLUS Chemiluminescent Substrate
Detection Biorad ChemiDoc
Results Summary I was looking to target Atxn7 isoform 2, named as Atxn7b in the literature, and referenced as ENSMUST00000223714.2 on Ensembl. (It differs only on the C-terminus compared to Atxn7a, isoform 1). Atxn7 is mainly in the nucleus and is between 100-130 kDa. An shRNA targeting Atxn7 displayed a decrease in Atxn7 abondance showing specificity of the antibody.

This antibody is highly specific and efficient, suitable for detecting STAT3 protein in rat colon by Western blot, with clear results.

Excellent, submitted by on
SKU M00007
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows Western blot results of the target protein STAT3 and the loading control Actin in the colon of normal rats, model rats, low/medium/high dose Chinese medicine treatment groups, and Western medicine treatment group. No differences were observed between the groups. The target bands are clear and distinct, and the experimental results are satisfactory.

This antibody is highly efficient and specific, suitable for Western blot detection of STAT3 (Phospho-Y705) protein in rat colon tissue, with only minor nonspecific bands observed.

Excellent, submitted by on
SKU P00007-2
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows representative Western blot results of the target protein STAT3 (Phospho-Y705) and the internal control Actin in rat colon tissue from the normal control group, disease model group, low-, medium-, and high-dose traditional Chinese medicine–treated groups, and the western medicine–treated group. The target bands are clear and well defined, and the experimental results are satisfactory.