| SKU | PA1050 |
|---|---|
| Application | Immunofluorenscence |
| Sample | Mouse spinal cord |
| Sample Processing Description | Mouse spinal cord fixed with 2% paraformaldehyde for 6–8 hours, dehydrated in 30% sucrose, embedded in OCT, and sectioned using a cryostat. |
| Primary Antibody | Anti-MBP9 antibody |
| Primary Incubation | 1:100, overnight at 4 ℃ |
| Blocking Agent | 3% BSA |
| Secondary Antibody | Anti-rabbit IgG-CY3 conjugated antibody. |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Detection | Immunofluorescence images were acquired using a confocal laser microscope (Leica SP8, Zeiss LSM 880 Airyscan). |
| Results Summary | BOSTER’s rabbit anti-MBP antibody (catalog BA0094) has high specificity and low background, enabling sensitive detection of demyelination and myelin regeneration processes, greatly facilitating this study. |