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Western blot of OCI-LY1 cells treated with SPHINX31 showed stable GAPDH expression with clear bands and clean background, demonstrating high specificity of A00227-1.

Excellent, submitted by on
SKU A00227-1
Application Western Blot
Sample human OCI-LY1 cellls
Sample Processing Description Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio, boiled for 10 minutes, and 15 μL of protein was loaded per well.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:5000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary In OCI-LY1 cells treated with different concentrations of SPHINX31 for 24 h, GAPDH expression remained stable with no significant differences, showing clear bands and a clean background without nonspecific signals.

The Anti-GAPDH antibody (A00227-1) demonstrated high sensitivity and clear WB bands in mouse intestinal tissue, offering excellent cost-effectiveness and reliability, and is highly recommended for use.

Excellent, submitted by on
SKU A00227-1
Application Western Blot
Sample mouse intesinal tissue
Sample Processing Description Mouse colon tissue was lysed in RIPA buffer containing a protease inhibitor cocktail. Protein concentration was determined using the Pierce™ BCA Protein Assay Kit, and equal amounts of protein were loaded after boiling denaturation.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody goat anti rabbit secondary antibodies
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary This antibody is highly sensitive, produces clear WB bands, is reusable, offers excellent cost-effectiveness, and demonstrates a clear advantage over similar international products, making it highly recommended for use.

In WB using β-tubulin antibody (Cat# A01857-1), a clear, specific band was observed in OCI-LY8 cells with clean background, showing stable expression after SRPK1 knockdown.

Excellent, submitted by on
SKU A01857-1
Application Western Blot
Sample human OCI-LY1 cells
Sample Processing Description Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio and boiled for 10 minutes. Fifteen microliters of each sample were loaded per well.
Other Reagents 5% Non-fat milk
Primary Antibody Beta Tubulin/TUBB Antibody
Primary Incubation 1:3000, overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL luminescent reagent, Imaging system:ChemiDoc MP
Results Summary The antibody is highly specific and efficient, with a clean background and no nonspecific bands. The target band has clear and well-defined edges.

Accurate localization and good imaging performance.

Excellent, submitted by on
SKU PB9093
Application Immunofluorenscence
Sample Mouse 4T1 cell xenograft tumor
Sample Processing Description Fixed in 4% paraformaldehyde for 48 hours, followed by paraffin embedding and sectioning.
Primary Incubation 1:200, overnight at 4 ℃
Blocking Agent Goat serum
Secondary Antibody DyLight 550-conjugated goat anti-rabbit antibody.
Secondary Incubation Incubate at room temperature for 1 hour
Detection Laser confocal microscopy
Results Summary The delivery time for antibodies is impressively fast — I usually receive the products within a week, which saves me a lot of valuable time. Both the pre-sales and after-sales support are efficient and reliable. Since my background is in chemistry, I often consulted Boster’s technical specialists about various details of biological experiments. They were always patient and thorough in their explanations, helping me avoid many detours during my experiments. Most importantly, these antibodies offer excellent value for money — they have strong binding performance, produce clear imaging results, and ensure a high experiment success rate, which has provided a solid foundation for my research.

The CLIPB4 antibody was efficiently used to detect CLIPB4 protein secreted in the mosquito hemolymph. The protein migrates at ~40 kDa in SDS-PAGE.

Excellent, submitted by on
CLIPB4 antibody Western blot result
SKU DZ33989-1
Application Western Blot
Sample β-galactosidase gene knockdown mosquito hemolymph, CLIPB4 knockdown mosquito hemolymph
Primary Incubation Overnight at 4°C
Secondary Incubation For 1 hour at room temperature
Secondary Antibody Dilution 1:3000
Detection Clarity Max Western ECL substrate
Results Summary Image showing the specificity of CLIPB4 antibody. Lane 1 includes hemolymph extracted from mosquitoes silenced for the bacterial β-galactosidase gene. Lane 2 contains hemolymph extracted from mosquitoes silenced for the CLIPB4 gene.

The CTL4 antibody was efficiently used to detect CTL4 protein secreted in the mosquito hemolymph. The protein migrates at ~15 kDa in SDS-PAGE.

Excellent, submitted by on
SKU DZ41074
Application Western Blot
Sample β-galactosidase gene knockdown mosquito hemolymph, CTL4 knockdown mosquito hemolymph
Primary Incubation +4°C overnight
Secondary Incubation For 1 hour at room temperature
Tertiary Incubation 1:1000
Detection Clarity Max western ECL substrate
Results Summary Image showing the specificity of CTL4 antibody. Lane 1 includes hemolymph extracted from mosquitoes silenced for the bacterial β-galactosidase gene. Lane 2 contains hemolymph extracted from mosquitoes silenced for the CTL4 gene.

The CLIPA14 antibody was efficiently used to detect CLIPA14 protein secreted in the mosquito hemolymph. The protein migrates at ~59 kDa in SDS-PAGE.

Excellent, submitted by on
SKU DZ41073
Application Western Blot
Sample β-galactosidase gene knockdown mosquito hemolymph, CLIPA14 knockdown mosquito hemolymph
Primary Incubation +4°C overnight
Secondary Incubation For 1 hour at room temperature
Tertiary Incubation 1:3000
Detection Clarity Max western ECL substrate
Results Summary Image showing the specificity of CLIPA14 antibody. Lane 1 includes hemolymph extracted from mosquitoes silenced for the bacterial β-galactosidase gene. Lane 2 contains hemolymph extracted from mosquitoes silenced for the CLIPA14 gene.