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Produced clear, sharp bands with no background. Excellent specificity and consistency across replicates. Ideal as a loading control for WB.

Excellent, submitted by on
SKU A01263
Application Western Blot
Sample Human 293T and A549 cell lysates, Mouse brain tissue
Sample Processing Description Cells were lysed in RIPA buffer containing protease inhibitors. Lysates were clarified by centrifugation and quantified using a BCA assay. 30–35 µg of protein was loaded per lane on a 5–20% SDS-PAGE gel and transferred to nitrocellulose membranes.
Primary Antibody Anti-beta Actin ACTB Antibody
Primary Incubation 1:1000, incubated overnight at 4°C.
Secondary Antibody Goat anti-rabbit IgG-HRP (Catalog # BA1054).
Secondary Incubation 1:5000 dilution, 1 hour at room temperature.
Other Reagents used 5% non-fat milk/TBST blocking buffer, ECL Plus substrate, Azure Biosystems c600 imaging system.
Detection Chemiluminescent detection. Strong single band observed at ~42 kDa, corresponding to beta actin.
Results Summary Produced clear, sharp bands with no background. Excellent specificity and consistency across replicates. Ideal as a loading control for WB.

The antibody is highly efficient and specific, showing a clear target band with no non-specific bands.

Excellent, submitted by on
SKU A01263
Application Western Blot
Sample HEK293T
Blocking Agent 5% Non-fat milk
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody Anti-Rabbit IgG Secondary Antibody, HRP-conjugated
Secondary Incubation Incubate at room temperature for 1 hour
Detection Azure Biosystems c600, ECL substrate
Results Summary I will definitely purchase BosterBio products again and recommend them to my classmates and colleagues.

Verification of Antibody on Recombinant Protein

--Hanjo Hellmann, Washington State University, School of Biological Sciences, Associate Professor

Excellent, submitted by on

Source: Biocompare.com

SKU DZ33980
Application Western Blot
Sample Recombinant protein expressed in and purified from E. coli
Detection Chemiluminescence

"We tested the antibody against recombinant GST-tagged BnaA07g20720D protein. As controls, we used another GST-tagged protein GST-MYB25, and GST alone. The antibody was well capable of detecting specifically BnaA07g20720D. We loaded ~100 ng of either GST:BnaA07g20720D, GST:MYB25, or GST alone, and the antibody was very specifically detecting BnaA07g20720D. It did not cross-react at all with the other two proteins. Highly specific. I would highly recommend the antibody for basic western-blot analysis of purified proteins. Probably one can significantly reduce the dilution to 1:10000 to still get a good result. Unfortunately, we did not have the opportunity with good controls to verify how the antibody works on whole plant extracts or for IP experiments."