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Western blot of OCI-LY1 cells treated with SPHINX31 showed stable GAPDH expression with clear bands and clean background, demonstrating high specificity of A00227-1.

Excellent, submitted by on
SKU A00227-1
Application Western Blot
Sample human OCI-LY1 cellls
Sample Processing Description Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio, boiled for 10 minutes, and 15 μL of protein was loaded per well.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:5000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary In OCI-LY1 cells treated with different concentrations of SPHINX31 for 24 h, GAPDH expression remained stable with no significant differences, showing clear bands and a clean background without nonspecific signals.

The Anti-GAPDH antibody (A00227-1) demonstrated high sensitivity and clear WB bands in mouse intestinal tissue, offering excellent cost-effectiveness and reliability, and is highly recommended for use.

Excellent, submitted by on
SKU A00227-1
Application Western Blot
Sample mouse intesinal tissue
Sample Processing Description Mouse colon tissue was lysed in RIPA buffer containing a protease inhibitor cocktail. Protein concentration was determined using the Pierce™ BCA Protein Assay Kit, and equal amounts of protein were loaded after boiling denaturation.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody goat anti rabbit secondary antibodies
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary This antibody is highly sensitive, produces clear WB bands, is reusable, offers excellent cost-effectiveness, and demonstrates a clear advantage over similar international products, making it highly recommended for use.

One major band was detected at the expected size (84 kDa), while multiple minor bands were detected in the egg lysate under the condition tested.

Excellent, submitted by on
SKU DZ41739
Application Western Blot
Sample egg lysate
Sample Processing Description Egg lysate was prepared and loaded onto a 10% polyacrylamide gel for electrophoresis.
Primary Antibody Anti-Purple sea urchin LOC581356 Antibody
Primary Incubation 1:2000, incubated overnight at 4°C.
Secondary Antibody Goat anti-rabbit IgG-HRP
Secondary Incubation 2 hour at room temperature.
Detection Chemiluminescent detection.
Results Summary One major band was detected at the expected size (84 kDa), while multiple minor bands were detected in the egg lysate under the condition tested.

A single band was detected at the expected size of 60kDa in the gastrula stage lysate.

Excellent, submitted by on
SKU DZ41738
Application Western Blot
Sample Embryo lysate
Sample Processing Description Embryonic lysate was prepared and loaded onto a 10% polyacrylamide gel for electrophoresis.
Primary Antibody Anti-Purple sea urchin LOC584590 Antibody
Primary Incubation 1:2000, incubated overnight at 4°C.
Secondary Antibody Goat anti-rabbit IgG-HRP
Secondary Incubation 2 hour at room temperature.
Detection Chemiluminescent detection.
Results Summary A single band was detected at the expected size of 60kDa in the gastrula stage lysate.

This antibody is highly efficient and specific, suitable for detecting AQP4 protein in rat colon by Western blot, with only minimal nonspecific bands.

Excellent, submitted by on
SKU PB9475
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody Aquaporin 4/AQP4 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows a schematic representation of Western blot results for the target protein AQP4 and the loading control Actin in rat colon across the normal, model, traditional Chinese medicine, and western medicine groups. The target bands are clear and distinct, and the experimental results are satisfactory.

Was able to detect tfap2a expression in the retinal ganglion cells and anterior segment at 3dpf.

Excellent, submitted by on
SKU DZ41119
Application Immunofluorescence
Sample Zebrafish retinal cryo-section
Sample Processing Description Embryos fixed in 4% PFA for 4h. Embryos washed in PBST and 30% then 50% sucrose. Embedded in OCT and cryo-sectioned at 20nm
Primary Antibody Zebrafish Tfap2a Antibody
Primary Incubation 1:100, overnight at 4 ℃
Detection Used a Nikon C2+ confocal microscope
Results Summary Was able to detect tfap2a expression in the retinal ganglion cells and anterior segment at 3dpf.

Thank you for all the work on this project

Excellent, submitted by on
SKU DZ01481
Application Immunofluorescence
Sample wildtype and KO mutant zebrafish retina
Primary Incubation 1:200
Blocking Agent 10% normal goat serum and 2% bovine serum albumin in PBS
Secondary Antibody Alexa Fluor 568 goat anti-rabbit was used in blocking solution.

Both Are Confirmed Specific In Zebrafish Using One Of Our KO Lines.

Excellent, submitted by on
SKU DZ4103
Application Immunofluorescence
Sample wildtype and KO mutant zebrafish retina
Primary Incubation 1:100
Blocking Agent 10% normal goat serum and 2% bovine serum albumin in PBS
Secondary Antibody Alexa Fluor 488 goat anti-rabbit and was used in a 1:800 dilution.

Both are confirmed specific in zebrafish using one of our KO lines.

Excellent, submitted by on
SKU DZ41032
Application Immunofluorescence
Sample wildtype and KO mutant zebrafish retina
Primary Incubation 1:100
Blocking Agent 10% normal goat serum and 2% bovine serum albumin in PBS
Secondary Antibody Alexa Fluor 568 goat anti-rabbit was used in a 1:800 dilution.

Beautiful Imaging of Zebrafish Eosinophils

Excellent, submitted by on

Source: Biocompare.com

SKU DZ41383
Application Immunofluorescence
Sample Zebrafish WKM, IPEX and FFPE tissue sections
Primary Incubation Overnight
Blocking Agent 3% goat serum in PBS
Secondary Incubation 1 hour
Tertiary Incubation None
Detection Fluorescence microscopy
DOI or PMID # https://doi.org/10.1101/2024.04.29.591640
Results Summary Antibody detects eosinophils and stains eosinophil granula. It is highly specific and can be used in dilutions such as 1:1000 for IF.

"We ordered this antibody as a custom antibody from Boster Bio to stain for zebrafish eosinophils in FFPE tissue sections and fixed cells. It works wonderfully: very bright, with no relevant background or off-target staining, and highly reproducible."