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Anti-C-Terminal CD44 Antibody for Western Blotting

Excellent, submitted by on

Source: Biocompare.com

SKU PA1021-2
Application Western Blot
Sample Tumor cell lysate
Primary Incubation Overnight at 8-10 degrees Celcius, with rocking, 1 ug/ml in 5% milk/BPS/Tw
Blocking Agent 5% milk in PBS/0.05% Tween-20 (5% milk/PBS/Tw)
Secondary Incubation Goat anti-Rabbit antibody conjugated with HRP at 1:3,000 in 5% milk/PBS/Tw
Tertiary Incubation HRP-bound secondary antibodies were detected by WestPico from ThermoScientific/Tw
Detection Chemiluminescence: West Pico from Thermo Scientific
Results Summary The antibody recognizes the expected ~80 kDa full-length CD44 and its low mol. wt. fragments containing C-terminal domain. The antibody is highly specific, produces "clean", definitive results; does not produce any non-specific bands. The antibody is sensitive and detects CD44 band when total protein per lane is loaded at 10-20 ug. The antibody is stable and could be re-used for blotting several times when stored in the original 5% milk/PBS/Tw solution at -20℃.

"The antibody was used to detect the full length and cleaved fragments of human transmembrane protein CD44. The rabbit antibody PA1021-2 is sensitive, i.e. detects CD44 protein bands under reducing conditions and also when tested material is loaded at low total protein per lane. The antibody is highly specific, i.e. does not recognize any bands of unknown nature on the membrane."

A Nice Antibody For Gp91phox Subunit Of NOX System

Excellent, submitted by on

Source: Biocompare.com

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SKU PA1667
Application Western Blot
Sample 22RV1 cytosolic and membrane fractions
Primary Incubation 1:1000, overnight at 4 degree
Blocking Agent 5% Milk
Secondary Incubation 1:5000, 1 hour RT.
Detection ECL
Results Summary Please refer our paper (Scientific Reports 6, Article number: 23135 (2016)doi:10.1038/srep23135).

"We used this antibody to study the inhibitory effect of GPE on hypoxia induced translocation of gp91phox subunit from cytosol to membrane in our recently published paper (Scientific Reports 6, Article number: 23135 (2016); doi:10.1038/srep23135). This antibody is very much specific in our in vitro system."

Successful MMP-9 measurements

Excellent, submitted by on

Source: Biocompare.com

SKU EK0465
Application Test the quantity of MMP-9 released from neutrophils after challenge with bacteria
Starting Material Human Neutrophils
Protocol Overview 1. collect supernatant from bacteria-challenged neutrophils2. Run samples on MMP-9 ELISA kit3. Read results on plate reader
Tips Frozen supernatants work just as well as fresh ones
Results Summary I highly recommend the Boster line of ELISA kits because of their cost and their effectiveness. I had one last round of experiments to run for a publication and we regrettably decided to purchase a lower cost kit from another company. I ran the same samples on two different plates from that company and even my controls were all over the place, resulting in a waste of time and money. This is when we purchased the Boster kit, and by only using one plate, I was able to see the real results of my experiment. My negative and positive controls had the necessary jump and my samples followed the expected trend.

"The instructions are clear, concise, and the process is more streamlined than other kits. I highly recommend investing in these kits compared to other, less expensive ones."

Anti-phospho Lyn (Y396) Rabbit Monoclonal Antibody

Excellent, submitted by on

Source: Biocompare.com

SKU P01424-1
Application Flow Cytometry
Sample B cells
Primary Incubation 0.25 µl/10^6 cells 50min RT
Blocking Agent FBS 5%
Secondary Incubation F(ab')2-Donkey anti-Rabbit IgG (H+L), PE 0.1 µl/10^6
Detection Flow Cytometry
Results Summary Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million cells with pLYN antibody (P01424-1) during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In red secondary antibody only and in blue primary anti-phospho lyn + secondary antibody.

A New and Reliable ELISA for Rat FABP4

Excellent, submitted by on

Source: Biocompare.com

SKU EK1573
Application ELISA
Starting Material Rat Plasma
Tips Kit suggested sample dilution is 1:50 but I used 1:25 and got results within standard range.
Results Summary We compared plasma FABP4 levels in pregnant and nonpregnant rats and results were consistent with reported studies.

"There were a number of ELISA kits for human and mice FABP4 but rat-specific kits were not easily available. We tried this Kit from Boster and are happy with the kit performance. The steps were easy to follow and standers and sample values were in an acceptable range."

User-Friendly & Reliable AGP1 ELISA Kit

Excellent, submitted by on

Source: Biocompare.com

SKU EK1486
Application Urinary Biomarker Discovery
Starting Material Urine
Tips Fast and good product.
Results Summary Orosomucoid-1 (AGP1) may have important effects on urological malignancies, both with its altering the pharmacokinetics of chemotherapeutics and its immunomodulatory and pro-angiogenicproperties.

"I have used other ELISA kits from different brands, but this specific ELISA kit from Booster is very user-friendly and fast. Consistent test results while working with many patients."

Anti-ULK2 Antibody Works Great for Western Blotting

Excellent, submitted by on

Source: Biocompare.com

SKU A05219
Application Western Blot
Sample HEK293T
Primary Incubation 1:5000 for 24hours at 4degrees celsius.
Blocking Agent Skim milk
Secondary Incubation 1 hour room temperature 1:3000
Detection ECL
Results Summary We silenced ULK1 and ULK2 genes in HEK293T cells, the antibody could differentiate the two proteins.

"Used for western blotting. The antibody worked good, used skim milk for blocking. It was used for investigating silenced HEK293T cells."

Thyroid Stimulating Hormone ELISA Kit From Boster Bio Works Well

Excellent, submitted by on

Source: Biocompare.com

SKU EK7098
Application Human TSH in dried blood spots
Starting Material Dried blood spots
Tips Use the 0 standard as a diluent, PBS works decently if not.
Results Summary The standard curve results consistently gave an excellent curve. No values on the curve or within our patient samples fell outside of the range.

"The purpose of this experiment was to quantify the level of TSH in a dried blood spot. The application of dried blood spots over whole blood or serum is to cater to remote patients and provide at-home healthcare."

Reliable Human T3 ELISA Kit From Boster

Excellent, submitted by on

Source: Biocompare.com

SKU EK7010
Application Human progesterone quantification in dried blood spots
Starting Material Dried Blood Spots
Tips Use the 0 standard as a diluent, don't include it on the standard curve.
Results Summary Our data concluded that dried blot spots can be used to quantify T3 levels in humans. The sensitivity remains relevant. Results are consistent and repeatable.

"This experiment was used to quantify the levels of T3 in humans from dried blood spots for the use of at-home test kits."

Superb Human Progesterone ELISA Kit From Boster

Excellent, submitted by on

Source: Biocompare.com

SKU EK7004
Application Human Progesterone saliva levels
Starting Material Saliva
Tips This is a competitive assay. If you use a blank well to subtract background noise, make sure to not add any reagents except stop.
Results Summary This product gave repeatable and reliable results. Results in the lower range (below 0.5) went OWR, consider adjusting the sensitivity.

"The goal of this experiment was to quantify the levels of progesterone in saliva for at-home test kits. In combination with other kits, we want to provide accurate healthcare to men and women."