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I was so thrilled that I took the photo below — it felt like a true treasure.

Excellent, submitted by on
SKU PB9234
Application ICC/IF
Sample Endothelial cells
Sample Processing Description Endothelial cells were seeded in collagen gel-treated chips for 12 hours.
Primary Antibody Anti-ZO1 tight junction protein/TJP1 Antibody Picoband®
Primary Incubation 1:200, overnight at 4 ℃
Blocking Agent Ready-to-use Goat Serum
Secondary Antibody Goat Anti-Rabbit IgG (H+L) Secondary Antibody, DyLight®488 Conjugated(BA1127)
Secondary Incubation Incubate at room temperature for 1 hour
Other reagents DAPI (AR1176)， Anti-fade mounting medium
Detection Fluorescence microscope
Results Summary At a critical stage of our manuscript preparation, we needed to stain tight junction proteins in endothelial cells. After trying primary antibodies from many companies without achieving satisfactory results, it was ultimately Boster’s product that solved the problem (Figure 5A). I was so excited that I took the photo below — treating it like a true treasure.

Highly prone to positive signals, with precise localization of expression

Excellent, submitted by on
MA1083 Immunofluorenscence
SKU MA1083
Application Immunofluorenscence
Sample Mouse MC-8 cells
Sample Processing Description 4% paraformaldehyde for 15 minutes
Primary Antibody Anti-PCNA Antibody (Monoclonal, PC 10)
Primary Incubation 1:1000, overnight at 4 ℃
Blocking Agent Goat serum
Secondary Antibody DyLight 550-conjugated goat anti-rabbit antibody.
Secondary Incubation Incubate at room temperature for 1 hour
Detection Laser confocal microscopy
Results Summary I will purchase Boster products again and recommend them to my classmates and colleagues.

This antibody is highly efficient and specific, with virtually no non-specific bands.

Excellent, submitted by on
A00284-1 Immunofluorenscence
SKU A00284-1
Application Immunofluorenscence
Sample Mouse cell climbing slides
Sample Processing Description Cells were directly lysed in NP40 lysis buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98°C. The samples were then loaded onto SDS-PAGE, with 20 μl of cell protein sample per lane.
Primary Antibody Anti-NF-kB p65/RELA Antibody Picoband®
Primary Incubation 1:2000, overnight at 4 ℃
Blocking Agent 5% Non-fat milk
Secondary Antibody HRP-conjugated Goat Anti-Rabbit IgG
Secondary Incubation Incubate at room temperature for 1 hour
Detection Imaging system: Tanon, ECL substrate
Results Summary I will purchase Boster products again and recommend them to my classmates and colleagues.

Highly prone to positive signals, with precise expression localization

Excellent, submitted by on
SKU M00656
Application Immunofluorenscence
Sample Mouse MC-8 cells
Sample Processing Description 4% paraformaldehyde for 15 minutes
Primary Antibody Anti-MMP14/Mt1 Mmp Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Blocking Agent Goat serum
Secondary Antibody DyLight 488-conjugated Goat Anti-Rabbit IgG.
Secondary Incubation Incubate at room temperature for 1 hour
Detection Laser confocal microscopy
Results Summary I will purchase Boster products again and recommend them to my classmates and colleagues.

BOSTER’s rabbit anti-MBP antibody (catalog BA0094) exhibits high specificity and low background, enabling sensitive detection of demyelination and myelin regeneration processes, greatly facilitating this study.

Excellent, submitted by on
SKU PA1050
Application Immunofluorenscence
Sample Mouse spinal cord
Sample Processing Description Mouse spinal cord fixed with 2% paraformaldehyde for 6–8 hours, dehydrated in 30% sucrose, embedded in OCT, and sectioned using a cryostat.
Primary Antibody Anti-MBP9 antibody
Primary Incubation 1:100, overnight at 4 ℃
Blocking Agent 3% BSA
Secondary Antibody Anti-rabbit IgG-CY3 conjugated antibody.
Secondary Incubation Incubate at room temperature for 1 hour
Detection Immunofluorescence images were acquired using a confocal laser microscope (Leica SP8, Zeiss LSM 880 Airyscan).
Results Summary BOSTER’s rabbit anti-MBP antibody (catalog BA0094) has high specificity and low background, enabling sensitive detection of demyelination and myelin regeneration processes, greatly facilitating this study.

Accurate localization and good imaging quality

Excellent, submitted by on
SKU A00066-1
Application Immunofluorenscence
Sample Mouse 4T1 cell climbing slides
Sample Processing Description Mouse 4T1 cells fixed with 4% paraformaldehyde for 15 minutes
Primary Antibody Anti-HMGB1 Antibody Picoband®
Primary Incubation 1:500, overnight at 4 ℃
Blocking Agent Goat serum
Secondary Antibody DyLight 550-conjugated goat anti-rabbit antibody.
Secondary Incubation Incubate at room temperature for 1 hour
Detection Laser confocal microscopy
Results Summary The ordering and delivery cycle of the antibodies is very short — I was able to receive the products within a week, which saved a lot of time. The pre-sales and after-sales services are convenient and efficient. Since my background is in chemistry, I consulted Boster’s technical team about many details of biological experiments. The technical specialists were patient and thorough in their analysis and explanations, which helped me avoid many detours during the actual experimental procedures. Most importantly, these antibodies offer a high cost-performance ratio, with good binding performance, excellent imaging quality, and a high experimental success rate, providing a solid foundation for conducting related experiments.

Accurate localization and good imaging quality

Excellent, submitted by on
SKU PB9640
Application Immunofluorenscence
Sample Mouse 4T1 cell climbing slides
Sample Processing Description Mouse 4T1 cells fixed with 4% paraformaldehyde for 15 minutes
Primary Antibody Anti-GRP78 antibody
Primary Incubation 1:400, overnight at 4 ℃
Blocking Agent Goat serum
Secondary Antibody DyLight 550-conjugated goat anti-rabbit antibody.
Secondary Incubation Incubate at room temperature for 1 hour
Detection Laser confocal microscopy
Results Summary The ordering and delivery cycle of the antibodies is short — I was able to receive the products within a week, which saved a lot of time. The pre-sales and after-sales services are convenient and efficient. Since my background is in chemistry, I consulted Boster’s technical staff about many details of biological experiments. The technical specialists were patient and helpful in their analysis and explanations, which allowed me to avoid many detours during the actual experimental procedures. Most importantly, these antibodies offer a high cost-performance ratio, with good binding performance, excellent imaging results, and a high experimental success rate, providing a solid foundation for conducting related experiments.

Accurate localization and good imaging quality

Excellent, submitted by on
SKU PA1239
Application Immunofluorenscence
Sample Rat Brain
Sample Processing Description Fixed in 4% paraformaldehyde for 48 hours, followed by paraffin embedding and sectioning.
Primary Antibody Anti-GFAP antibody
Primary Incubation 1:500, overnight at 4 ℃
Blocking Agent Goat serum
Secondary Antibody DyLight 550-conjugated goat anti-rabbit antibody.
Secondary Incubation Incubate at room temperature for 1 hour
Detection Laser confocal microscopy
Results Summary During the experiment, this primary antibody can be conveniently used with the complimentary antibody diluent provided. The results obtained showed good quality and reproducibility, with no false-positive signals. Overall, this primary antibody offers excellent cost performance and provides key data and strong support for publication.

This antibody exhibits high specificity, an excellent signal-to-noise ratio, and clear, stable staining, and has been successfully applied in immunofluorescent labeling of mouse cartilage tissue.

Excellent, submitted by on
SKU PA2141-1
Application Immunofluorescence
Sample mouse cartilage tissue
Sample Processing Description Freshly dissected bone samples were first fixed overnight in 4% paraformaldehyde (PFA), followed by decalcification for 3 days in 10% ethylenediaminetetraacetic acid (EDTA) solution, and then dehydrated for 1 day in 30% sucrose solution. The bone samples were subsequently embedded in optimal cutting temperature compound (OCT; Sakura, Cat. No. OCT-4583) and cryosectioned at a thickness of 10 μm using a Leica CryoJane cryostat.
Primary Antibody Anti-Collagen II/COL2A1 Antibody Picoband®
Primary Incubation 1:100, overnight at 4 °C.
Secondary Antibody donkey anti-rabbit Alexa Fluor 555 Secondary Antibody
Secondary Incubation 1:500, Incubate at room temperature for 1 hour
Other Reagents used PBS with 10% horse serum, DAPI
Detection fluorescence microscope (Leica)
Results Summary I will purchase BosterBio products again and recommend them to my classmates and colleagues.

Accurate localization and good imaging performance.

Excellent, submitted by on
SKU PB9093
Application Immunofluorenscence
Sample Mouse 4T1 cell xenograft tumor
Sample Processing Description Fixed in 4% paraformaldehyde for 48 hours, followed by paraffin embedding and sectioning.
Primary Incubation 1:200, overnight at 4 ℃
Blocking Agent Goat serum
Secondary Antibody DyLight 550-conjugated goat anti-rabbit antibody.
Secondary Incubation Incubate at room temperature for 1 hour
Detection Laser confocal microscopy
Results Summary The delivery time for antibodies is impressively fast — I usually receive the products within a week, which saves me a lot of valuable time. Both the pre-sales and after-sales support are efficient and reliable. Since my background is in chemistry, I often consulted Boster’s technical specialists about various details of biological experiments. They were always patient and thorough in their explanations, helping me avoid many detours during my experiments. Most importantly, these antibodies offer excellent value for money — they have strong binding performance, produce clear imaging results, and ensure a high experiment success rate, which has provided a solid foundation for my research.