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Source: Biocompare.com
| SKU | DZ02095 |
|---|---|
| Application | Immunofluorescence |
| Sample | Zebrafish embryos 5dpf |
| Detection | Confocal Microscopy |
"Antibody labels the retina outer segments and inner nuclear layers as expected based on in situ hybridization studies. The staining is clean. A zebrafish-specific antibody for Siah1 that actually WORKS!"
Source: Biocompare.com
| SKU | DZ12892 |
|---|---|
| Application | Immunofluorescence |
| Sample | Zebrafish embryos 24hpf |
| Detection | Confocal Microscopy |
"Zebrafish-specific antibody. Used in whole mount IF on 24hpf zebrafish embryos. The signal is not precisely clean, but we do observe nlz2 expression in the ventral region of the retina as expected from in situ hybridization results. This antibody shows some promising results in whole mount applications using 24hpf embryos. Applications with cryosections may be more precise."
| SKU | PB9435 |
|---|---|
| Application | Immunohistochemistry (paraffin-embedded) |
| Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
| Sample | Rat Brain Tissue |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Ab Incubation | 37°C for 30 minutes |
| Primary Ab Incubation diluent | 5% BSA in TBS |
| Primary Ab Concentration | 1ug/ml |
| Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
| Secondary Ab Dilution | The kit was ready to use, no dilution needed |
| Secondary Ab Incubation | 4°C overnight |
| SKU | PB9008 |
|---|---|
| Application | Immunohistochemistry (Paraffin-embedded) |
| Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
| Sample | Human Ovary |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Ab Incubation | 4°C overnight |
| Primary Ab Incubation diluent | 5% BSA in TBS |
| Primary Ab Concentration | 1ug/ml |
| Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
| Secondary Ab Dilution | The kit was ready to use, no dilution needed |
| Secondary Ab Incubation | 4°C overnight |
| SKU | PB9026 |
|---|---|
| Application | Immunohistochemistry (Paraffin-embedded) |
| Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
| Sample | Human tonsil |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Ab Incubation | 37°C for 30 minutes |
| Primary Ab Incubation diluent | 5% BSA in TBS |
| Primary Ab Concentration | 2ug/ml |
| Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
| Secondary Ab Dilution | The kit was ready to use, no dilution needed |
| Secondary Ab Incubation | 4°C overnight |
| SKU | PB10059 |
|---|---|
| Application | Immunofluorescence (IF) |
| Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
| Sample | Human rectal |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Ab Incubation | 4°C overnight |
| Primary Ab Incubation diluent | 5% BSA in TBS |
| Primary Ab Concentration | 1ug/ml |
| Secondary Antibody | SABC
kit from Boster Bio, (SA1022 |
| Secondary Ab Dilution | The kit was ready to use, no dilution needed |
| Secondary Ab Incubation | at 37°C for 30 min |
Source: Biocompare.com
| SKU | PA1302-1 |
|---|---|
| Application | Immunohistochemistry |
| Sample | Rat spinal cord |
| Detection | Fluorescent microscope |
"I purchased this product to stain rat spinal cords exposed to injury. The injured spinal cords showed apoptotic cells obviously. Injured spinal cord sample showed high apoptosis compared to control (uninjured) spinal cord. Excellent, reliable product and easy to use."
Source: Biocompare.com
| SKU | PB9271 |
|---|---|
| Application | Western Blot |
| Sample | Human Tissue |
| Detection | Fluorescent based |
"We are comparing the expression of the TRPC5 protein in psychological symptoms characterized in a human tissue lysate. This Anti-TRPC5 antibody worked perfectly in detection in human tissue. Perfect band obtained at desired molecular weight. No extra bands. I recommend."
Source: Biocompare.com
| SKU | PA1385 |
|---|---|
| Application | Western Blot |
| Sample | Human NSC cell lysates |
| Detection | ECL chemiluminescence |
"We study neurodegeneration in our lab and I was looking for an antibody for human TIMP-1. The antibody detects a band at 28kD, which is the predicted molecular weight of TIMP-1. Good antibody for TIMP-1, cleaner than other antibodies I have tested. In summary, this antibody is good for human samples and I highly recommend it."
| SKU | PB9094 |
|---|---|
| Application | Immunohistochemistry (paraffin-embedded) |
| Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
| Sample | Human Bowel |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Ab Incubation | 37°C for 30 min |
| Primary Ab Incubation diluent | 5% BSA in TBS |
| Primary Ab Concentration | 1ug/ml |
| Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
| Secondary Ab Dilution | The kit was ready to use, no dilution needed |
| Secondary Ab Incubation | at 37°C for 30 min |