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Thank you for all the work on this project

Excellent, submitted by on
SKU DZ01481
Application Immunofluorescence
Sample wildtype and KO mutant zebrafish retina
Primary Incubation 1:200
Blocking Agent 10% normal goat serum and 2% bovine serum albumin in PBS
Secondary Antibody Alexa Fluor 568 goat anti-rabbit was used in blocking solution.

The antibody performs efficiently and specifically, with very few nonspecific bands.

Excellent, submitted by on
WB result image
SKU A03213-2
Application Western Blot
Sample MCF-7 cell
Sample Processing Description Cells were directly lysed in NP-40 buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98 °C. Then, 20 µL of protein sample was loaded per lane onto SDS-PAGE.
Primary Antibody UBA6 Antibody
Primary Incubation 1:1000, overnight at 4 °C
Blocking Agent 5% Non-fat milk
Secondary Antibody HRP-conjugated Goat Anti-Rabbit IgG
Secondary Incubation Incubate at room temperature for 1 hour
Detection Signal was developed using ECL substrate on a Tanon system.
Results Summary The antibody performs efficiently and specifically, with very few nonspecific bands.

This antibody shows excellent consistency and reproducibility across batches, ensuring reliable and stable experimental results. Its performance remains consistent across different experiments, providing strong technical support for our research.

Excellent, submitted by on
PA1079 wb
SKU PA1079
Application Western Blot
Sample Mouse lung tissue
Sample Processing Description Tissue samples were directly lysed in RIPA buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98 °C. Load 20 µL of protein sample per lane onto SDS-PAGE.
Primary Incubation The membrane was incubated with TNF-α primary antibodies (1:1000) overnight at 4 °C.
Secondary Antibody HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody
Secondary Incubation Incubate at room temperature for 1 hour
Other Reagents used 5% Non-fat milk
Detection Signal was developed using ECL substrate on an ChemiDoc MP system.
Results Summary This antibody shows excellent consistency and reproducibility across batches, ensuring reliable and stable experimental results. Its performance remains consistent across different experiments, providing strong technical support for our research.

This antibody shows excellent consistency and reproducibility across batches, ensuring reliable and stable experimental results. Its performance remains consistent across different experiments, providing strong technical support for our research.

Excellent, submitted by on
SKU M03918
Application Western Blot
Sample Mouse brown adipose tissue
Sample Processing Description Tissue and cell proteins were extracted using RIPA buffer. After BCA quantification, 5× loading buffer was added, and the samples were boiled for 5 minutes for denaturation.
Primary Incubation The membrane was incubated with the PLIN1 primary antibodies (1:1000) overnight at 4 °C.
Secondary Antibody Goat Anti-Rabbit IgG Antibody (1:5000)
Secondary Incubation Incubate at room temperature for 1 hour
Other Reagents used Non-fat milk
Detection Signal was developed using ECL substrate
Results Summary This antibody shows excellent consistency and reproducibility across batches, ensuring reliable and stable experimental results. Its performance remains consistent across different experiments, providing strong technical support for our research.

The antibody is highly efficient and specific, showing a clear target band with no nonspecific bands.

Excellent, submitted by on
SKU M00220-1
Application Western Blot
Sample Mouse lung cancer tissue
Sample Processing Description Tissue samples were directly lysed in RIPA buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98 °C. Load 20 µL of protein sample per lane onto SDS-PAGE.
Primary Incubation The membrane was incubated with the CD86 primary antibodies (1:2000) overnight at 4 °C.
Secondary Antibody HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody
Secondary Incubation Incubate at room temperature for 1 hour
Other Reagents used 5% non-fat milk
Detection Signal was developed using ECL substrate on an Tanon system.
Results Summary The antibody is highly efficient and specific, showing a clear target band with no nonspecific bands. I will definitely continue using BosterBio products and will recommend them to my classmates and colleagues.

IL-6 and IL-1β levels in the pancreas are key for assessing the anti-inflammatory effect of this nanomedicine. Antibodies from two previous suppliers showed poor specificity, while BosterBio antibodies proved highly specific, potent, and cost-effective.

Excellent, submitted by on
SKU M00101-1
Application Western Blot
Sample Mouse pancreatic acinar cells
Sample Processing Description After centrifugation, collect the supernatant. Take a small portion for protein quantification using the BCA assay. Mix the remaining protein solution with an equal volume of loading buffer and denature in a 100°C water bath for 5 mins.
Primary Incubation The membrane was incubated with the IL-6 and IL-1β primary antibodies (1:1000) overnight at 4 °C.
Secondary Antibody HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody
Secondary Incubation Incubate at room temperature for 1 hour
Other Reagents used Protein-Free Blocking Buffer
Detection Signal was developed using ECL substrate on a ChemiDoc MP system.
Results Summary IL-6 and IL-1β levels in the pancreas are key for assessing the anti-inflammatory effect of this nanomedicine. Antibodies from two previous suppliers showed poor specificity, while BosterBio antibodies proved highly specific, potent, and cost-effective. In our subsequent experiments, our group continued to use BosterBio products, which proved to be highly reliable and provided solid support for the publication of several articles.

Works very well in Western blot for detecting pChk2 in human HeLa cells.

Excellent, submitted by on
SKU P00277-1
Application Western Blot
Sample Hela cell
Sample Processing Description After 12 h of viral infection, cells were lysed in RIPA buffer with PMSF and heated in 1× protein loading buffer for 10 min to denature proteins.
Primary Incubation The membrane was incubated with the CHEK2 (Phospho-T68) primary antibody (1:2000) overnight at 3 hours.
Secondary Antibody HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody
Secondary Incubation Incubate at room temperature for 1 hour
Other Reagents used None
Detection Signal was developed using ECL substrate on an ChemiDoc MP system.
Results Summary Works very well in Western blot for detecting pChk2 in human HeLa cells.

Works very well in Western blot for rat enteric glial cells and mouse FGL-2 protein, with only slight nonspecific bands.

Excellent, submitted by on
SKU A06303-3
Application Western Blot
Sample rat enteric glial cells and mouse tissue
Sample Processing Description Samples (5 µL per lane) were run on 10% resolving and stacking gels at 80 V for 20 min, then 150 V for 70 min, and transferred at 400 mA for 100 min.
Primary Incubation The membrane was incubated with the FGL-2 primary antibody (1:1000) overnight at 4 °C.
Secondary Antibody HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody
Secondary Incubation Incubate at room temperature for 2 hour
Other Reagents used 5% non-fat milk
Detection Signal was developed using ECL substrate on an ImageQuant LAS4000mini system.
Results Summary Works very well in Western blot for rat enteric glial cells and mouse FGL-2 protein, with only slight nonspecific bands.

The antibody shows high efficiency and specificity in Western blot detection of hemolymph cell proteins from Pacific oyster, with only slight nonspecific bands.

Excellent, submitted by on
SKU PB9148
Application Western Blot
Sample hemolymph of the Pacific oyster
Sample Processing Description Add 10 µL of hemolymph cells to each lane and directly lyse in 1× Laemmli sample buffer. After adding β-mercaptoethanol, load the samples onto SDS-PAGE.
Primary Incubation The membrane was incubated with the MyD88 primary antibody (1:1000) overnight at 4 °C.
Secondary Antibody HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody
Secondary Incubation Incubate at room temperature for 1 hour
Other Reagents used Milk
Detection Signal was developed using ECL substrate on a ChemiDoc MP system.
Results Summary The antibody shows high efficiency and specificity in Western blot detection of hemolymph cell proteins from Pacific oyster, with only slight nonspecific bands.

High-Quality Serinc5 Antibody From Boster Bio Produces a Clear Band

Excellent, submitted by on

Source: Biocompare.com

SKU A06603
Application Western Blot
Sample Ripa buffer lysis transfected HEK293 cell
Primary Incubation +4°C overnight
Blocking Agent 5% milk in 1X TBST
Secondary Incubation 30 mins
Tertiary Incubation 1:1000
Detection Clarity Western ECL Substrate
Results Summary The antibody is excellent at recognizing and expressing the protein at the correct position. I have tried samples from different vendors, but only this vendor offers high-quality products.