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ADO Antibody Works in Western Blot

--Hua Jiang, Pediatrics, University of Colorado Denver, Research Associate

Excellent, submitted by on

Source: Biocompare.com

SKU PB10032
Applications Western Blot
Sample Human liver, rat liver, mouse liver, mouse kidney, and mouse brain
Detection Typhoon 940

"We are studying the metabolism of amino acid and we are interested in knowing if the pathway involved this protein has changed under the background of a knockout mouse. The western blot image for this antibody looks clean and the signal looks strong. The band shown on western blot is at the right size and the intensity is strong enough. Equally important, the price is generally lower than other companies. This antibody works ok in western blot experiments with human, rat, and mouse tissues."

It works far better compared to other vendors

--Saikat Chakraborty, PhD, Feinberg School of Medicine, Northwestern University

Excellent, submitted by on

Source: Customer Feedback Submission

SKU PA1373
Applications Western Blot
Sample Mouse brain lysate
Detection Licor Imaging and ImageJ

"We have tested PA1373 trial sample in our lab and it works far better compared to other vendors. We are extremely happy to get the results. We looked for 5HT2A expression in various brain regions. Our bands show clear expression level of 5HT2A. We now plan to buy this antibody for all our laboratory needs."

PB9920 successfully extracted rat osteocalcin from rat EDTA plasma

--Chad Borges, Arizona State University, Biodesign Virginia G. Piper Center for Personalized Diagnostics, Assistant Professor

Excellent, submitted by on

Source: Customer Feedback Submission

SKU PB9920
Applications Picomole scale immunoprecipitation
Sample Rat EDTA plasma
Detection Bruker maXis 4G mass spectrometer (LC-MS; Q-TOF)

"The immobilized antibody successfully extracted rat osteocalcin from rat EDTA plasma. The results shown align very well with mass and known posttranslational modifications of rat osteocalcin."

BosterBio Sparcl1 Works

--Ruby C, NEUROSCIENCE, TUFTS UNIVERSITY, RESEARCH ASSISTANT

Excellent, submitted by on

Source: Biocompare.com

SKU PA2088
Applications Immunohistochemistry
Sample Mouse astrocytes
Detection Confocal/Fluorescent Scope

"The general aim of the experiment was to determine molecular differences of astrocytes in the central nervous system. We wanted to see if there are different expression levels of Sparcl1 in different regions of the mouse brain as a way to compare and possibly validate qPCR data. The product was found on One World Lab and it worked so we decided to buy a greater quantity as opposed to a trial size. The bottom line: Good."

Good enough for publishing

--Chun-Chih Tseng, NORRIS CANCER CENTER, UNIVERSITY OF SOUTHERN CALIFORNIA, RESEARCH ASSISTANT

Excellent, submitted by on

Source: Biocompare.com

SKU PB9333
Applications Western Blot
Sample Human breast cancer cell line
Detection Bio-Rad imager

"I used this anti-CD44 C-terminus antibody to detect cleaved/non-cleaved CD44 product. The results for Western blot are okay. The signal (1:1500) is not super strong but good enough to see clear bands. This antibody could detect a band the same size as another antibody that I used against the extracellular region of CD44, indicating this antibody could detect CD44. CD44 has multiple spliced isoforms. The WB result showed multiple bands, some of the bands I am interested in overlapped with the bands generated by another CD44 antibody targeting the extracellular domains. So, the specificity of this antibody is probably okay. The intensity of bands are acceptable, good enough for publishing. Relatively inexpnsive, okay specificity. Good enough to detect CD44 C-term."

We got bands as expected...

--Christina Klinger, DEPT. OF TRANSLATIONAL AND REGENERATIVE MEDICINE, UNIVERSITY OF ARIZONA, MANAGER OF LABORATORY OPERATIONS

Excellent, submitted by on

Source: Biocompare.com

SKU PA1129-2
Applications Western Blot
Sample Mouse lung lysates
Detection SuperSignal West Femto Maximum Sensitivity Substrate

"We sought to see differences in expression of EPAS1 in protein from mouse lung samples. We selected this product because we have used other antibodies from Boster in the past with success. We got bands as expected, but they were not indicative of good conjugation as they were very light. We believe this is because we did not use the recommended Enhanced Chemiluminscent kit with anti-rabbit IgG (EK1002), so we will repeat the experiment with this chemi kit before concluding whether the antibody worked or not. This antibody should work using the chemiluminescent kit recommended...Heed the recommendation of the website and use the chemi kit suggested for high quality visualization."

Anti GRK5 (PB 9708) is useful, good.

--Yongzhen Guo, EMERGENCY MEDICINE, THOMAS JEFFERSON UNIVERSITY, RESEARCH ASSISTANT

Excellent, submitted by on

Source: Biocompare.com

SKU PB9708
Applications Western Blot
Sample Rat endothelial cells
Detection Kodak Image Station 4000R Pro;Thermo Scientific West Femto Maximum Sensitivity Substrate(#34096)

"After using this antibody for western blot to analyze rat endothelial cells' protein, I found PB9708 Anti-GRK5 antibody worked very well. The blot of 68 Kda can be seen, however, we can see another 3 blots at least. In a word, Anti GRK5 (PB 9708) is useful and good. The blot of 68 Kda can be detected."

Reliable and robust

--R.P., BIOLOGY, PENN STATE UNIVERSITY, TECHNICIAN

Excellent, submitted by on
Rat il 6 elisa kit

Source: Biocompare.com

SKU PA1340
Applications Western Blot
Sample Cell lysate
Detection Enhanced chemiluminescence

"This is an excellent antibody to endoplasmin in HC11 cells. Clean, reliable detection with very little if any background. Great antibody for Westerns. Could probably be optimized for 1 hour primary incubation."

Working Antibody for Versican

--Gang Ye, BIOLOGY, YORK UNIVERSITY, RESEARCH ASSOCIATE/LAB MANAGER

Excellent, submitted by on

Source: Biocompare.com

SKU PB9453
Applications Western Blot
Sample Human cell lines
Detection ECL

"Since versican has several isoforms, the current antibodies targeting versican on the market is very tricky. Most of them didn't give us a reasonable result. Some of them only detect a band at 70 KD, which is unlikely a potent component of versican protein. Bosterbio claims this antibody dectects a band over 250 KD. Since versican is a very big protein. It sounds this is a real antibody. After testing, we found we can see a clear detected band around 245 KD. We realized that this might be the true result...Although 245 KD is still smaller than the versican core protein, it is likely the true result of versican dectection."

High specificity and stability

--Elena Deryugina, CELL AND MOLECULAR BIOLOGY, THE SCRIPPS RESEARCH INSTITUTE, ASSISTANT PROFESSOR

Excellent, submitted by on

Source: Biocompare.com

SKU PB9333
Applications Western Blot
Sample Tumor cell lysate and recombinant protein
Detection SuperSignal West Pico Chemiluminescent Substrate

"The rabbit antibody PB9333 from Boster produced very good results in western blotting with the lysate of human CD44-positive cancer cells as well as with the recombinant N-terminal protein. The detection was specific, resulting only in the protein bands of expected mol. weights. The antibody is also capable of detecting shed CD44 in conditioned medium. High specificity and stability. I would highly recommend this antibody for detection of CD44 in human cells by western blotting."