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Anti-Smoothened/SMO Antibody for Flow Cytometry

--Yair Fisher, GliXogen Therapeutics, CSO

Excellent, submitted by on

Source: Customer Feedback Submission

SKU A00451-1
Application Flow Cytometry
Sample C3H10T1/2 mouse cell line

"Overlay histogram showing C3H10T1/2 cells stained with A00451-1 (red line). The cells were blocked with 5% normal goat serum, then incubated with rabbit anti-Smoothened/SMO Antibody (A00451-1, 1:50, 2 μg/0.5x10^6 cells) for 30 min at RT. Alexa Fluor® 647 AffiniPure F(ab')₂ Fragment Goat Anti-Rabbit IgG (H+L) (1:250) was used as a secondary antibody for 30 minutes at RT. Isotype control antibody (blue line) used under the same conditions. Unlabelled sample (yellow line) was also used as a control."

Good Zebrafish Mef2 Antibody from Boster Bio

--Jingli Cao, Assistant Professor, Weill Cornell Medical College

Excellent, submitted by on
SKU DZ01398-1
Application Immunofluorescence
Sample Zebrafish heart
Detection Confocal Microscopy

"Mef2a was detected in a cryosection of zebrafish heart using the rabbit anti-Mef2a antibody (cat# DZ01398-1, 1:200, 4 degrees overnight). AlexaFluor546 Conjugated Goat Anti-Rabbit IgG was used as a secondary antibody at 1:200 dilution and incubated for 1.5 h at room temperature."

Good Mouse IL-12 ELISA Kit

--Daisy Dai University of Miami Surgery Lab Director / Manager

Excellent, submitted by on

Source: Biocompare.com

SKU EK0422
Application ELISA
Starting Material Crispr MEK and STAT3 KO CAFs
Tips This kit doesn't provide PC

"We investigate how alterations in RAS and STAT3 signaling pathways affect the tumor stroma and immune microenvironment in pancreatic cancer. This kit was used to detect the IL-12 level in CM from Crispr MEK and STAT3 KO CAFs. The whole process was about 7 hours including sample preparation."

Immunohistochemistry for Anti-TPR Antibody

Excellent, submitted by on
SKU A00695-1
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Mouse Brain
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 37 °C for 30 minutes
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 2 ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Immunocytochemistry for Anti-E Cadherin 1/CDH1 Antibody

Excellent, submitted by on
PB9561 Immunohistochemistry

The antibody worked perfectly, Great sensitivity

SKU PB9561
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
SampleMouse Liver
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 2ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation 4°C overnight

Immunohistochemistry for Anti-CD5 Antibody

Excellent, submitted by on
SKU A00480-2
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Tonsil
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 0.5 ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Immunocytochemistry for Anti-HnRNP D/AUF1/HNRNPD Antibody

Excellent, submitted by on
SKU A09982-1
Application Immunocytochemistry
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Colorectal tissue
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 37°C for 30 minutes
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Great PKC antibody for IHC

Excellent, submitted by on
We used Anti-PKC Alpha Picoband™ Antibody (SKU: PB9318) for IHC experiment from mouse intestine tissues. Clear positive staining with the right subcellular locations. Super easy to determine the dilution ratio! Great quality.

Immunohistochemistry for Anti-Mannose Receptor/MRC1

Excellent, submitted by on
SKU A02285-2
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Mouse skin
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 37°C for 30 minutes
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

A Good PKC Alpha Antibody for IP and WB

--Ramaz Geguchadze, University of New England, Biomedical Sciences, Research Scientist

Excellent, submitted by on

Source: Biocompare.com

SKU PB9318
Application Western Blot
Sample Dorsal root ganglia (neuronal cells)
Detection CyDye

"We used Anti-PKC Alpha Picoband™ Antibody for immunoprecipitation experiment from neuronal cells in order to characterize PKC Alpha expression in different conditions. Strong positive band with right mass. Works great without optimization! Good quality and ready for publish Ab."