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Successful MMP-9 measurements

Excellent, submitted by on

Source: Biocompare.com

SKU EK0465
Application Test the quantity of MMP-9 released from neutrophils after challenge with bacteria
Starting Material Human Neutrophils
Protocol Overview 1. collect supernatant from bacteria-challenged neutrophils2. Run samples on MMP-9 ELISA kit3. Read results on plate reader
Tips Frozen supernatants work just as well as fresh ones
Results Summary I highly recommend the Boster line of ELISA kits because of their cost and their effectiveness. I had one last round of experiments to run for a publication and we regrettably decided to purchase a lower cost kit from another company. I ran the same samples on two different plates from that company and even my controls were all over the place, resulting in a waste of time and money. This is when we purchased the Boster kit, and by only using one plate, I was able to see the real results of my experiment. My negative and positive controls had the necessary jump and my samples followed the expected trend.

"The instructions are clear, concise, and the process is more streamlined than other kits. I highly recommend investing in these kits compared to other, less expensive ones."

Anti-phospho Lyn (Y396) Rabbit Monoclonal Antibody

Excellent, submitted by on

Source: Biocompare.com

SKU P01424-1
Application Flow Cytometry
Sample B cells
Primary Incubation 0.25 µl/10^6 cells 50min RT
Blocking Agent FBS 5%
Secondary Incubation F(ab')2-Donkey anti-Rabbit IgG (H+L), PE 0.1 µl/10^6
Detection Flow Cytometry
Results Summary Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million cells with pLYN antibody (P01424-1) during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In red secondary antibody only and in blue primary anti-phospho lyn + secondary antibody.

Mettl23 Detection in Cell Lines

Excellent, submitted by on

Source: Biocompare.com

SKU DZ41142
Application Western Blot
Sample Human cell lines
Primary Incubation 1:500 with 1% BSA in TBS, incubated overnight, at 4oC, with shaking
Blocking Agent 5% BSA in TBS
Secondary Incubation Licor IRDye 800 Goat anti Rabbit diluted 1:10000 with 1% BSA in TBS, incubated for 1 hour at RT , with shaking
Detection Licor Odyssey IR Scanner
Additional Notes The antibody has very good stability at room temperature (transit took over 2 weeks and the ice packs had long melted before delivery).
Results Summary The antibody gave a detectable band at the expected size for all cell lines tested with no non-specific bands.

"This antibody was used to determine the level of protein expression in various cell lines in order to set up two groups (high vs low expression). This antibody was custom made because numerous suppliers have either stopped their production for this antibody or quality/specificity was low."

User-Friendly & Reliable AGP1 ELISA Kit

Excellent, submitted by on

Source: Biocompare.com

SKU EK1486
Application Urinary Biomarker Discovery
Starting Material Urine
Tips Fast and good product.
Results Summary Orosomucoid-1 (AGP1) may have important effects on urological malignancies, both with its altering the pharmacokinetics of chemotherapeutics and its immunomodulatory and pro-angiogenicproperties.

"I have used other ELISA kits from different brands, but this specific ELISA kit from Booster is very user-friendly and fast. Consistent test results while working with many patients."

Very Clean Anti-Lactoferrin/LTF Antibody Picoband

Excellent, submitted by on

Source: Biocompare.com

SKU A00633-1
Application Western Blot
Sample Mouse Neutrophils
Primary Incubation 1:2000 1 hr
Blocking Agent 5% BSA
Secondary Incubation 1:3000
Detection HRP
Results Summary Nicely compared total LTF in neutrophils to degranulated LTF. In the image below, Lane 1: Total LTF from mouse neutrophil cell lysates. Lane 2: degranulated LTF. Ratio 1:20.

"Nicely detects degranulated LTF in supernatant mouse neutrophils stimulated with bacteria. Used at 1:2000 in 5% BSA."

MTHFD2 Antibody for Western Blot

Excellent, submitted by on

Source: Biocompare.com

SKU M06465
Application Western Blot
Sample Hello cell and mouse tissues
Primary Incubation 1:2000 dilution at 4oC overnight
Blocking Agent 5% non-fat milk
Secondary Incubation 1:2500 dilution at RT for 1 hour
Detection Fluorescence
Results Summary This antibody works in WB with tested samples. The signal is strong enough to get a decent image. It works for human and mouse samples.

"This antibody of MTHFD2 is good for WB with mouse tissue and human cell. Signal is strong and the background is ok."

Anti-ULK2 Antibody Works Great for Western Blotting

Excellent, submitted by on

Source: Biocompare.com

SKU A05219
Application Western Blot
Sample HEK293T
Primary Incubation 1:5000 for 24hours at 4degrees celsius.
Blocking Agent Skim milk
Secondary Incubation 1 hour room temperature 1:3000
Detection ECL
Results Summary We silenced ULK1 and ULK2 genes in HEK293T cells, the antibody could differentiate the two proteins.

"Used for western blotting. The antibody worked good, used skim milk for blocking. It was used for investigating silenced HEK293T cells."

Thyroid Stimulating Hormone ELISA Kit From Boster Bio Works Well

Excellent, submitted by on

Source: Biocompare.com

SKU EK7098
Application Human TSH in dried blood spots
Starting Material Dried blood spots
Tips Use the 0 standard as a diluent, PBS works decently if not.
Results Summary The standard curve results consistently gave an excellent curve. No values on the curve or within our patient samples fell outside of the range.

"The purpose of this experiment was to quantify the level of TSH in a dried blood spot. The application of dried blood spots over whole blood or serum is to cater to remote patients and provide at-home healthcare."

Reliable Human T3 ELISA Kit From Boster

Excellent, submitted by on

Source: Biocompare.com

SKU EK7010
Application Human progesterone quantification in dried blood spots
Starting Material Dried Blood Spots
Tips Use the 0 standard as a diluent, don't include it on the standard curve.
Results Summary Our data concluded that dried blot spots can be used to quantify T3 levels in humans. The sensitivity remains relevant. Results are consistent and repeatable.

"This experiment was used to quantify the levels of T3 in humans from dried blood spots for the use of at-home test kits."

Superb Human Progesterone ELISA Kit From Boster

Excellent, submitted by on

Source: Biocompare.com

SKU EK7004
Application Human Progesterone saliva levels
Starting Material Saliva
Tips This is a competitive assay. If you use a blank well to subtract background noise, make sure to not add any reagents except stop.
Results Summary This product gave repeatable and reliable results. Results in the lower range (below 0.5) went OWR, consider adjusting the sensitivity.

"The goal of this experiment was to quantify the levels of progesterone in saliva for at-home test kits. In combination with other kits, we want to provide accurate healthcare to men and women."