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Works very well in Western blot for rat enteric glial cells and mouse FGL-2 protein, with only slight nonspecific bands.

Excellent, submitted by on
SKU A06303-3
Application Western Blot
Sample rat enteric glial cells and mouse tissue
Sample Processing Description Samples (5 µL per lane) were run on 10% resolving and stacking gels at 80 V for 20 min, then 150 V for 70 min, and transferred at 400 mA for 100 min.
Primary Incubation The membrane was incubated with the FGL-2 primary antibody (1:1000) overnight at 4 °C.
Secondary Antibody HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody
Secondary Incubation Incubate at room temperature for 2 hour
Other Reagents used 5% non-fat milk
Detection Signal was developed using ECL substrate on an ImageQuant LAS4000mini system.
Results Summary Works very well in Western blot for rat enteric glial cells and mouse FGL-2 protein, with only slight nonspecific bands.

The antibody shows high efficiency and specificity in Western blot detection of hemolymph cell proteins from Pacific oyster, with only slight nonspecific bands.

Excellent, submitted by on
SKU PB9148
Application Western Blot
Sample hemolymph of the Pacific oyster
Sample Processing Description Add 10 µL of hemolymph cells to each lane and directly lyse in 1× Laemmli sample buffer. After adding β-mercaptoethanol, load the samples onto SDS-PAGE.
Primary Incubation The membrane was incubated with the MyD88 primary antibody (1:1000) overnight at 4 °C.
Secondary Antibody HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody
Secondary Incubation Incubate at room temperature for 1 hour
Other Reagents used Milk
Detection Signal was developed using ECL substrate on a ChemiDoc MP system.
Results Summary The antibody shows high efficiency and specificity in Western blot detection of hemolymph cell proteins from Pacific oyster, with only slight nonspecific bands.

High-Quality Serinc5 Antibody From Boster Bio Produces a Clear Band

Excellent, submitted by on

Source: Biocompare.com

SKU A06603
Application Western Blot
Sample Ripa buffer lysis transfected HEK293 cell
Primary Incubation +4°C overnight
Blocking Agent 5% milk in 1X TBST
Secondary Incubation 30 mins
Tertiary Incubation 1:1000
Detection Clarity Western ECL Substrate
Results Summary The antibody is excellent at recognizing and expressing the protein at the correct position. I have tried samples from different vendors, but only this vendor offers high-quality products.

Anti-C-Terminal CD44 Antibody for Western Blotting

Excellent, submitted by on

Source: Biocompare.com

SKU PA1021-2
Application Western Blot
Sample Tumor cell lysate
Primary Incubation Overnight at 8-10 degrees Celcius, with rocking, 1 ug/ml in 5% milk/BPS/Tw
Blocking Agent 5% milk in PBS/0.05% Tween-20 (5% milk/PBS/Tw)
Secondary Incubation Goat anti-Rabbit antibody conjugated with HRP at 1:3,000 in 5% milk/PBS/Tw
Tertiary Incubation HRP-bound secondary antibodies were detected by WestPico from ThermoScientific/Tw
Detection Chemiluminescence: West Pico from Thermo Scientific
Results Summary The antibody recognizes the expected ~80 kDa full-length CD44 and its low mol. wt. fragments containing C-terminal domain. The antibody is highly specific, produces "clean", definitive results; does not produce any non-specific bands. The antibody is sensitive and detects CD44 band when total protein per lane is loaded at 10-20 ug. The antibody is stable and could be re-used for blotting several times when stored in the original 5% milk/PBS/Tw solution at -20℃.

"The antibody was used to detect the full length and cleaved fragments of human transmembrane protein CD44. The rabbit antibody PA1021-2 is sensitive, i.e. detects CD44 protein bands under reducing conditions and also when tested material is loaded at low total protein per lane. The antibody is highly specific, i.e. does not recognize any bands of unknown nature on the membrane."

A Nice Antibody For Gp91phox Subunit Of NOX System

Excellent, submitted by on

Source: Biocompare.com

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SKU PA1667
Application Western Blot
Sample 22RV1 cytosolic and membrane fractions
Primary Incubation 1:1000, overnight at 4 degree
Blocking Agent 5% Milk
Secondary Incubation 1:5000, 1 hour RT.
Detection ECL
Results Summary Please refer our paper (Scientific Reports 6, Article number: 23135 (2016)doi:10.1038/srep23135).

"We used this antibody to study the inhibitory effect of GPE on hypoxia induced translocation of gp91phox subunit from cytosol to membrane in our recently published paper (Scientific Reports 6, Article number: 23135 (2016); doi:10.1038/srep23135). This antibody is very much specific in our in vitro system."

Successful MMP-9 measurements

Excellent, submitted by on

Source: Biocompare.com

SKU EK0465
Application Test the quantity of MMP-9 released from neutrophils after challenge with bacteria
Starting Material Human Neutrophils
Protocol Overview 1. collect supernatant from bacteria-challenged neutrophils2. Run samples on MMP-9 ELISA kit3. Read results on plate reader
Tips Frozen supernatants work just as well as fresh ones
Results Summary I highly recommend the Boster line of ELISA kits because of their cost and their effectiveness. I had one last round of experiments to run for a publication and we regrettably decided to purchase a lower cost kit from another company. I ran the same samples on two different plates from that company and even my controls were all over the place, resulting in a waste of time and money. This is when we purchased the Boster kit, and by only using one plate, I was able to see the real results of my experiment. My negative and positive controls had the necessary jump and my samples followed the expected trend.

"The instructions are clear, concise, and the process is more streamlined than other kits. I highly recommend investing in these kits compared to other, less expensive ones."

Anti-phospho Lyn (Y396) Rabbit Monoclonal Antibody

Excellent, submitted by on

Source: Biocompare.com

SKU P01424-1
Application Flow Cytometry
Sample B cells
Primary Incubation 0.25 µl/10^6 cells 50min RT
Blocking Agent FBS 5%
Secondary Incubation F(ab')2-Donkey anti-Rabbit IgG (H+L), PE 0.1 µl/10^6
Detection Flow Cytometry
Results Summary Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million cells with pLYN antibody (P01424-1) during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In red secondary antibody only and in blue primary anti-phospho lyn + secondary antibody.

Mettl23 Detection in Cell Lines

Excellent, submitted by on

Source: Biocompare.com

SKU DZ41142
Application Western Blot
Sample Human cell lines
Primary Incubation 1:500 with 1% BSA in TBS, incubated overnight, at 4oC, with shaking
Blocking Agent 5% BSA in TBS
Secondary Incubation Licor IRDye 800 Goat anti Rabbit diluted 1:10000 with 1% BSA in TBS, incubated for 1 hour at RT , with shaking
Detection Licor Odyssey IR Scanner
Additional Notes The antibody has very good stability at room temperature (transit took over 2 weeks and the ice packs had long melted before delivery).
Results Summary The antibody gave a detectable band at the expected size for all cell lines tested with no non-specific bands.

"This antibody was used to determine the level of protein expression in various cell lines in order to set up two groups (high vs low expression). This antibody was custom made because numerous suppliers have either stopped their production for this antibody or quality/specificity was low."

User-Friendly & Reliable AGP1 ELISA Kit

Excellent, submitted by on

Source: Biocompare.com

SKU EK1486
Application Urinary Biomarker Discovery
Starting Material Urine
Tips Fast and good product.
Results Summary Orosomucoid-1 (AGP1) may have important effects on urological malignancies, both with its altering the pharmacokinetics of chemotherapeutics and its immunomodulatory and pro-angiogenicproperties.

"I have used other ELISA kits from different brands, but this specific ELISA kit from Booster is very user-friendly and fast. Consistent test results while working with many patients."

Very Clean Anti-Lactoferrin/LTF Antibody Picoband

Excellent, submitted by on

Source: Biocompare.com

SKU A00633-1
Application Western Blot
Sample Mouse Neutrophils
Primary Incubation 1:2000 1 hr
Blocking Agent 5% BSA
Secondary Incubation 1:3000
Detection HRP
Results Summary Nicely compared total LTF in neutrophils to degranulated LTF. In the image below, Lane 1: Total LTF from mouse neutrophil cell lysates. Lane 2: degranulated LTF. Ratio 1:20.

"Nicely detects degranulated LTF in supernatant mouse neutrophils stimulated with bacteria. Used at 1:2000 in 5% BSA."