Customer Testimonials

Source: Biocompare.com

"The goal of this experiment is to quantify the levels of human T4 in dried blood spots from at-home test kits. This assay in combination with other hormone tests is aimed at providing accurate and timely healthcare to patients remotely."

Source: Biocompare.com

"The experiment goal was to quantify the levels of testosterone in human saliva with this booster kit designed for blood or serum samples. The aim is to provide at-home test kits for remote patients using a non-invasive collection method."

Boster bio Iba1 1:100 PFA (3-4) - cortex 12x - with scale bar

Boster bio Iba1 1:100 PFA (3-4) - cortex 25x - with scale bar

Images that were made from fresh frozen cryostat sections, were thaw-mounted onto microscope slides. Mounted sections were later post-fixed with 300 µL of 4% paraformaldehyde (PFA) in PBS at room temperature for 10 minutes. After fixation and prior to incubation with antibodies, slides were washed 3 times with 300 µL of 1X PBS with 0.01% sodium azide for 3 minutes per rinse, followed by permeabilization with 300 µL of 0.3% Triton X-100 in 1X PBS with 0.01% sodium azide for 30 minutes at room temperature, then blocking with 700 µL of 4% donkey serum diluted in 1X PBS with 0.01% sodium azide + Triton X-100 (blocking buffer) for 30 minutes at room temperature. Slides were incubated with 325 µL of Iba1 (1:100). Concentrations of 1:100 were achieved by diluting 10 µL of antibody in 1,000 µL of blocking buffer; concentrations of 1:250 were achieved by diluting 4 µL of antibody in 1,000 µL of blocking buffer. Sections were incubated overnight at 4 ˚C. On the next day, slides were washed 3 times with 300 µL of 1X PBS with 0.01% sodium azide for 3 minutes per rinse, then incubated with the secondary antibodies at room temperature, in the dark, for 1 hour. Washing step was repeated then slides were left in the dark to dry. Mounting media was added to cover slip the sections. Slides were kept in the dark at 4 ˚C prior to imaging.

Boster bio GFAP 1:100 PFA (7-2) - cortex 12x - with scale bar

Boster bio GFAP 1:100 PFA (7-2) - cortex 20x - with scale bar

Boster bio GFAP 1:250 12x (free-floating)

Images that were made from fresh frozen cryostat sections, were thaw-mounted onto microscope slides. Mounted sections were later post-fixed with 300 µL of 4% paraformaldehyde (PFA) in PBS at room temperature for 10 minutes. After fixation and prior to incubation with antibodies, slides were washed 3 times with 300 µL of 1X PBS with 0.01% sodium azide for 3 minutes per rinse, followed by permeabilization with 300 µL of 0.3% Triton X-100 in 1X PBS with 0.01% sodium azide for 30 minutes at room temperature, then blocking with 700 µL of 4% donkey serum diluted in 1X PBS with 0.01% sodium azide + Triton X-100 (blocking buffer) for 30 minutes at room temperature. Slides were incubated with 325 µL of GFAP (1:100). Concentrations of 1:100 were achieved by diluting 10 µL of antibody in 1,000 µL of blocking buffer; concentrations of 1:250 were achieved by diluting 4 µL of antibody in 1,000 µL of blocking buffer. Sections were incubated overnight at 4 ˚C. On the next day, slides were washed 3 times with 300 µL of 1X PBS with 0.01% sodium azide for 3 minutes per rinse, then incubated with the secondary antibodies at room temperature, in the dark, for 1 hour. Washing step was repeated then slides were left in the dark to dry. Mounting media was added to cover slip the sections. Slides were kept in the dark at 4 ˚C prior to imaging.

Immunohistochemistry was also performed with free-floating sections, which were exposed to the same primary antibodies diluted (1:250) in blocking buffer overnight and, subsequently, exposed to secondary antibodies diluted in blocking buffer for 1 hour, following the same procedure as the mounted sections. After incubation, sections were washed 3 times with 300 µL of 1X PBS with 0.01% sodium azide for 3 minutes per rinse and transferred with a painting brush to a container filled with 1X PBS with 0.01% sodium azide, from which they were mounted onto slides. Slides were left to dry in the dark, after which mounting media was added to cover slip the sections. As with slide-mounted sections, these slides were kept in the dark at 4 ˚C prior to imaging.

Source: Customer Feedback Submission

"Overlay histogram showing C3H10T1/2 cells stained with A00451-1 (red line). The cells were blocked with 5% normal goat serum, then incubated with rabbit anti-Smoothened/SMO Antibody (A00451-1, 1:50, 2 μg/0.5x10^6 cells) for 30 min at RT. Alexa Fluor® 647 AffiniPure F(ab')₂ Fragment Goat Anti-Rabbit IgG (H+L) (1:250) was used as a secondary antibody for 30 minutes at RT. Isotype control antibody (blue line) used under the same conditions. Unlabelled sample (yellow line) was also used as a control."

Source: Biocompare.com

"I used this antibody for western blotting and immunoprecipitation. It works well in both of them. Very good antibody."

Source: Biocompare.com

"Positive:(1) Very straight forward and easy to use kits.(2) Accurate results(3) Removable well-strips so experiments can be staggered.Negative:(1) Price point - could be cheaper(2) Reagents have to be used within 1 month upon defrosting."