Customer Testimonials

Source: Biocompare.com

"Nicely detects degranulated LTF in supernatant mouse neutrophils stimulated with bacteria. Used at 1:2000 in 5% BSA."

Source: Biocompare.com

"This antibody of MTHFD2 is good for WB with mouse tissue and human cell. Signal is strong and the background is ok."

Source: Biocompare.com

"Used for western blotting. The antibody worked good, used skim milk for blocking. It was used for investigating silenced HEK293T cells."

Source: Biocompare.com

"The purpose of this experiment was to quantify the level of TSH in a dried blood spot. The application of dried blood spots over whole blood or serum is to cater to remote patients and provide at-home healthcare."

Source: Biocompare.com

"This experiment was used to quantify the levels of T3 in humans from dried blood spots for the use of at-home test kits."

Source: Biocompare.com

"The goal of this experiment was to quantify the levels of progesterone in saliva for at-home test kits. In combination with other kits, we want to provide accurate healthcare to men and women."

Source: Biocompare.com

"The goal of this experiment is to quantify the levels of human T4 in dried blood spots from at-home test kits. This assay in combination with other hormone tests is aimed at providing accurate and timely healthcare to patients remotely."

Source: Biocompare.com

"The experiment goal was to quantify the levels of testosterone in human saliva with this booster kit designed for blood or serum samples. The aim is to provide at-home test kits for remote patients using a non-invasive collection method."

Source: Biocompare.com

"We ordered this antibody as a custom antibody from Boster Bio to stain for zebrafish eosinophils in FFPE tissue sections and fixed cells. It works wonderfully: very bright, with no relevant background or off-target staining, and highly reproducible."

Boster bio Iba1 1:100 PFA (3-4) - cortex 12x - with scale bar

Boster bio Iba1 1:100 PFA (3-4) - cortex 25x - with scale bar

Images that were made from fresh frozen cryostat sections, were thaw-mounted onto microscope slides. Mounted sections were later post-fixed with 300 µL of 4% paraformaldehyde (PFA) in PBS at room temperature for 10 minutes. After fixation and prior to incubation with antibodies, slides were washed 3 times with 300 µL of 1X PBS with 0.01% sodium azide for 3 minutes per rinse, followed by permeabilization with 300 µL of 0.3% Triton X-100 in 1X PBS with 0.01% sodium azide for 30 minutes at room temperature, then blocking with 700 µL of 4% donkey serum diluted in 1X PBS with 0.01% sodium azide + Triton X-100 (blocking buffer) for 30 minutes at room temperature. Slides were incubated with 325 µL of Iba1 (1:100). Concentrations of 1:100 were achieved by diluting 10 µL of antibody in 1,000 µL of blocking buffer; concentrations of 1:250 were achieved by diluting 4 µL of antibody in 1,000 µL of blocking buffer. Sections were incubated overnight at 4 ˚C. On the next day, slides were washed 3 times with 300 µL of 1X PBS with 0.01% sodium azide for 3 minutes per rinse, then incubated with the secondary antibodies at room temperature, in the dark, for 1 hour. Washing step was repeated then slides were left in the dark to dry. Mounting media was added to cover slip the sections. Slides were kept in the dark at 4 ˚C prior to imaging.