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Peer Reviews from Real Labs

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Filter by category, reactivity, and host to find the most relevant peer results.

Immunocytochemistry for Anti-E Cadherin 1/CDH1 Antibody

Excellent, submitted by on
PB9561 Immunohistochemistry

The antibody worked perfectly, Great sensitivity

SKU PB9561
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
SampleMouse Liver
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 2ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation 4°C overnight

Immunohistochemistry for Anti-E Cadherin 1 CDH1 Antibody

Excellent, submitted by on
SKU M00063-2
Application Immunohistochemistry (Paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Oesophagus
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 2ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Immunohistochemistry for Anti-CD5 Antibody

Excellent, submitted by on
SKU A00480-2
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Tonsil
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 0.5 ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Immunocytochemistry for Anti-HnRNP D/AUF1/HNRNPD Antibody

Excellent, submitted by on
SKU A09982-1
Application Immunocytochemistry
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Colorectal tissue
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 37°C for 30 minutes
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Immunohistochemistry Review For Anti-CD147 Emmprin BSG Antibody

Excellent, submitted by on
SKU A00248-1
Application Immunohistochemistry (paraffin embedded)
Blocking step 5% BSA as blocking agent for 30 min at 37°C
Sample Placenta
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Great PKC antibody for IHC

Excellent, submitted by on
We used Anti-PKC Alpha Picoband™ Antibody (SKU: PB9318) for IHC experiment from mouse intestine tissues. Clear positive staining with the right subcellular locations. Super easy to determine the dilution ratio! Great quality.

Immunohistochemistry for Anti-Mannose Receptor/MRC1

Excellent, submitted by on
SKU A02285-2
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Mouse skin
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 37°C for 30 minutes
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Verification of Antibody on Recombinant Protein

--Hanjo Hellmann, Washington State University, School of Biological Sciences, Associate Professor

Excellent, submitted by on

Source: Biocompare.com

SKU DZ33980
Application Western Blot
Sample Recombinant protein expressed in and purified from E. coli
Detection Chemiluminescence

"We tested the antibody against recombinant GST-tagged BnaA07g20720D protein. As controls, we used another GST-tagged protein GST-MYB25, and GST alone. The antibody was well capable of detecting specifically BnaA07g20720D. We loaded ~100 ng of either GST:BnaA07g20720D, GST:MYB25, or GST alone, and the antibody was very specifically detecting BnaA07g20720D. It did not cross-react at all with the other two proteins. Highly specific. I would highly recommend the antibody for basic western-blot analysis of purified proteins. Probably one can significantly reduce the dilution to 1:10000 to still get a good result. Unfortunately, we did not have the opportunity with good controls to verify how the antibody works on whole plant extracts or for IP experiments."

A Good PKC Alpha Antibody for IP and WB

--Ramaz Geguchadze, University of New England, Biomedical Sciences, Research Scientist

Excellent, submitted by on

Source: Biocompare.com

SKU PB9318
Application Western Blot
Sample Dorsal root ganglia (neuronal cells)
Detection CyDye

"We used Anti-PKC Alpha Picoband™ Antibody for immunoprecipitation experiment from neuronal cells in order to characterize PKC Alpha expression in different conditions. Strong positive band with right mass. Works great without optimization! Good quality and ready for publish Ab."

Immunohistochemistry for Anti-VE-Cadherin-5 CDH5-Antibody

Excellent, submitted by on
SKU A02632-1
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Liver
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C Overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 2ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min