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Peer Reviews from Real Labs

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Immunofluorescence for Anti-CG6353 Antibody

--Amin Ghabrial  Pathology and Cell Biology  Columbia University  Principal Investigator

Excellent, submitted by on

Source: Biocompare.com

"My lab is characterizing a mutant we named disjointed. The disjointed gene is also known as CG6353 or Archease. We have used this antibody for IF, and it appears to work well. It gives us a staining pattern for the endogenous protein that matches the pattern we see with an HA-tagged fusion protein detected by anti-HA staining."

SKU DZ33921-1
Application Immunofluorescence
Sample Drosophila larvae
Primary Incubation   1:100 at 4 degrees overnight
Blocking Agent 4% horse serum
Secondary Incubation   1:1000 anti-Rb-A488
Tertiary Incubation   N/A
Detection IF
Results Summary Antibody staining reveals subcellular localization of the protein. In salivary gland and trachea, localization was primarily nuclear.

Anti-Smoothened/SMO Antibody for Flow Cytometry

--Yair Fisher, GliXogen Therapeutics, CSO

Excellent, submitted by on

Source: Customer Feedback Submission

SKU A00451-1
Application Flow Cytometry
Sample C3H10T1/2 mouse cell line

"Overlay histogram showing C3H10T1/2 cells stained with A00451-1 (red line). The cells were blocked with 5% normal goat serum, then incubated with rabbit anti-Smoothened/SMO Antibody (A00451-1, 1:50, 2 μg/0.5x10^6 cells) for 30 min at RT. Alexa Fluor® 647 AffiniPure F(ab')₂ Fragment Goat Anti-Rabbit IgG (H+L) (1:250) was used as a secondary antibody for 30 minutes at RT. Isotype control antibody (blue line) used under the same conditions. Unlabelled sample (yellow line) was also used as a control."

Western blot for Polyclonal Anti-Drosophila melanogaster (Fruit fly) emp Antibody

--Yuzuru Imai, Department of Research for Parkinson's Disease Juntendo University Graduate School of Medicine

Excellent, submitted by on
SKU DZ41152
Application Western blot
Sample Drosophila melanogaster (head and thorax)
Detection Immobilon Forte Western HRP substrate

"There are non-specific bands and immunostaining may not be possible, but Western blotting is sufficient with this antibody."

Immunohistochemistry for Anti-CD31/PECAM1 Antibody

Excellent, submitted by on
SKU M01513-4
Application Immunohistochemistry (Paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Lung
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 37°C for 30 minutes
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Good GATA3 Antibody from BosterBio

--Xiangrong Geng Internal medicine University of Michigan Postdoctoral Researcher

Excellent, submitted by on

Source: Biocompare.com

SKU M00593
Application Western Blot
Sample HEK293T
Detection ECL

"I used this antibody for western blotting and immunoprecipitation. It works well in both of them. Very good antibody."

Easy to Use Human Laminin ELISA Kit

--John Ong, University of Cambridge Engineering Graduate Student

Excellent, submitted by on

Source: Biocompare.com

SKU EK0434
Application Testing laminin concentration in hydrogel
Starting Material Hydrogel
Tips Supernatant used for ELISA

"Positive:(1) Very straight forward and easy to use kits.(2) Accurate results(3) Removable well-strips so experiments can be staggered.Negative:(1) Price point - could be cheaper(2) Reagents have to be used within 1 month upon defrosting."

Immunohistochemistry for Anti-TPR Antibody

Excellent, submitted by on
SKU A00695-1
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Mouse Brain
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 37 °C for 30 minutes
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 2 ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Immunohistochemistry review for CD147 Emmprin BSG antibody

Excellent, submitted by on
SKU A00248-1
Application Immunohistochemistry (paraffin embedded)
Blocking step 5% BSA as blocking agent for 30 min at 37°C
Sample Prostate cancer
Fixative Fixed with 4% paraformaldehyde
Primary Incubation 4°C overnight
Primary Incubation diluent 5% BSA in TBS
Primary Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Dilution The kit was ready to use, no dilution needed
Secondary Incubation at 37°C for 30 min

Immunocytochemistry for Anti-E Cadherin 1/CDH1 Antibody

Excellent, submitted by on
PB9561 Immunohistochemistry

The antibody worked perfectly, Great sensitivity

SKU PB9561
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
SampleMouse Liver
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 2ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation 4°C overnight

Immunohistochemistry for Anti-E Cadherin 1 CDH1 Antibody

Excellent, submitted by on
SKU M00063-2
Application Immunohistochemistry (Paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Oesophagus
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 2ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min