• Compare Products

  • Table of Contents

Customer Testimonials

Peer Reviews from Real Labs

Browse peer-submitted results linked to specific products—ratings, images, and practical notes included. Contribute your own result to strengthen the evidence base and claim rewards after verification.

Share Your Review Browse Reviews ↓

Video Interviews

See real workflows and decision points—what researchers tried, what worked, and why. Share your story in a short interview and claim rewards!

Get Interview Rewards

Filter by category, reactivity, and host to find the most relevant peer results.

The Anti-α-SMA antibody (Cat# BM3902) shows clear and specific bands with low background in WB analysis of mouse heart tissues across different ages, with results consistent with expected trends.

Excellent, submitted by on
SKU M01072-1
Application Western Blot
Sample mouse cardiac tissue
Sample Processing Description Mouse cardiac tissues were collected from 3-, 6-, 12-, and 18-month-old mice, and total protein was extracted.
Other Reagents RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:10000, 1 h at RT
Detection Substrate: ECL substrate
Imaging system: ChemiDoc MP
Results Summary This experiment aimed to investigate the changes in α-SMA expression in mouse cardiac tissues from 3-, 6-, 12-, and 18-month-old mice (representing young, middle-aged, and aged stages). The results show that α-SMA levels are generally similar across different ages, with a slight but not significant increase as age progresses.

Western blot using Anti-ER/ESR1 Antibody (M00057-2) in mouse brain tissues showed clear, specific ESR1 bands across normal, disease model, and AB drug-treated groups, with β‑actin as a consistent loading control.

Excellent, submitted by on
SKU M00057-2
Application Western Blot
Sample mouse brain tissue
Sample Processing Description Mouse brain tissues were lysed in RIPA buffer containing a protease inhibitor cocktail at 4°C for 2 hours, centrifuged to collect the supernatant, and protein concentration was determined. After adjusting the concentration, samples were mixed with 5× protein loading buffer, denatured by heating at 95–100°C for 10 minutes, and 15 μL of protein was loaded per lane for SDS-PAGE.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody goat anti rabbit secondary antibodies
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary This antibody is highly sensitive, produces clear WB bands, is reusable, offers excellent cost-effectiveness, and demonstrates a clear advantage over similar international products, making it highly recommended for use.

Anti-PRLR Antibody (PA2087) demonstrated clear and specific detection of PRLR in mouse brain tissue by Western blot, with distinct differences observed among the control, model, and AB treatment groups.

Excellent, submitted by on
SKU PA2087
Application Western Blot
Sample mouse brain tissue
Sample Processing Description Mouse brain tissues were lysed in RIPA buffer containing a protease inhibitor cocktail at 4 °C for 2 hours. After centrifugation, the supernatant was collected for protein quantification. The protein concentration was adjusted accordingly, mixed with 5× protein loading buffer, and denatured by heating for 10 minutes. Then, 15 μl of protein sample was loaded per lane for electrophoresis.
Other Reagents blocking buffer
Primary Antibody Prolactin Receptor/PRLR Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:2000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary The figure shows representative Western blot results of PRLR and the internal control β-actin in brain tissues from normal mice, the model group, and mice treated with low and high doses of AB. The antibody produced clear bands, and distinct differences among the experimental groups were clearly observed.

In WB using Anti-CDK1 (Phospho-T450) antibody (Cat# PB9533-50 µL), CDK1 expression in rat colon was increased in the model group and most effectively reduced in the high-dose herbal treatment group, with clear and well-defined target bands.

Excellent, submitted by on
SKU PB9533
Application Western Blot
Sample rat colon tissue
Sample Processing Description Samples were lysed in RIPA buffer containing PMSF protease inhibitor (100:1) for 10 min, centrifuged at 12,000 rpm for 15 min, and the supernatant was mixed with 5× loading buffer, boiled at 100 °C for 10 min, and loaded onto SDS-PAGE.
Other Reagents 5% Non-fat milk
Primary Antibody CDK1 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The image shows WB results of CDK1 and the loading control Actin in rat colon across different groups; CDK1 expression was elevated in the model group and most effectively reduced in the high-dose herbal treatment group, with clear and distinct target bands.

In this Western blot experiment using Anti-AQP1 antibody (Cat# BM5035) on MADB106 rat mammary carcinoma cells, AQP1 protein levels were markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor.

Excellent, submitted by on
SKU M00865
Application Western blot
Sample MADB106 rat mammary carcinoma cells
Sample Processing Description MADB106 cells under normal culture conditions , MADB106 cells treated with agonist , MADB106 cells treated with inhibitor
Other ReagentsRIPA Lysis Buffer, Protease Inhibitor, Resolving Gel Solution ,Transfer Buffer ,Blocking Buffer
Primary Antibody AQP1 Rabbit Monoclonal Antibody
Primary Incubation 1:3000, overnight at 4 ℃
Secondary Antibody 1:10,000, HRP-conjugated Goat Anti-Rabbit IgG
Secondary Incubation 1h at RT
Detection Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary AQP1 (Aquaporin 1) is the first discovered water channel protein, whose primary function is efficient water transport. Recent studies have revealed that AQP1 plays a critical role in cancer progression, promoting tumor growth in breast cancer by enhancing angiogenesis and cell migration. In this experiment, AQP1 protein levels were markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor.

The Anti-c-FOS antibody (M00297) showed clear and specific immunofluorescence staining in mouse brain, with markedly increased c-Fos expression in neurons of the acute ischemic region compared to normal brain.

Excellent, submitted by on
SKU M00297
Application Immunocytochemistry
Sample mouse brain
Sample Processing Description Normal brain tissue and brain tissue from a cerebral infarction model
Other ReagentsGoat serum, DAB Chromogenic Solution, Anti-fade mounting medium
Primary Antibody c-Fos Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody DyLight 594 Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 45 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary c-Fos is a marker of activated neural cells, especially neurons. During the acute phase of cerebral infarction, as a strong stress and injury stimulus, it drives c-Fos expression in the brain—particularly in the peri-infarct region—far higher than in normal resting brain. The experimental results show clear staining of positive cells, and the expression levels are consistent with expectations.

The Anti-FAP antibody (M00422) showed clear and specific IHC staining in human hepatocellular carcinoma, with low expression in adjacent normal tissue and strong expression in tumor stroma.

Excellent, submitted by on
SKU M00422
Application Immunohistochemistry
Sample human liver cancer
Sample Processing Description Collected clinical surgical human hepatocellular carcinoma and adjacent peritumoral tissues were fixed in formalin and embedded in paraffin.
Other ReagentsGoat serum, DAB Chromogenic Solution
Primary Antibody FAP1 Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Two-step IHC kit (Immunohistochemistry kit
Secondary Incubation 30 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary FAP (fibroblast activation protein) expression is mainly associated with stromal cells in the tumor microenvironment and is very low in normal human tissues. Its role in hepatocellular carcinoma (HCC) is complex, but overall it functions as an important pro-tumor factor. High FAP expression is associated with poor prognosis. It can remodel the extracellular matrix, promote tumor invasion and metastasis, and suppress anti-tumor immunity. In this study, FAP showed low expression in peritumoral tissues (close to normal tissue) and high expression in the tumor stroma of HCC.

The Anti-TH antibody (M01917) demonstrated excellent specificity and clear fluorescence staining in IF analysis of paraffin-embedded mouse striatum sections, accurately labeling dopaminergic neurons with minimal background.

Excellent, submitted by on
SKU M01917
Application Immunofluorescence
Sample mouse brain
Sample Processing Description Sagittal sections of mouse striatum were fixed in formaldehyde for 48 hours and paraffin-embedded.
Other ReagentsGoat serum，DAPI，Anti-fade mounting medium
Primary Antibody Iba1 Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody 1:500, Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro594 Conjugated
Secondary Incubation 45 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary TH is a marker of catecholaminergic neurons and can specifically label and identify dopaminergic, noradrenergic, and adrenergic neurons. In this experiment, TH immunofluorescence was performed to observe the distribution of dopaminergic neurons in the striatum. The results show clear staining and precise localization.

In an immunofluorescence experiment using normally cultured Caco-2 cells, the ZO-1 antibody (PB9234) showed clear and specific membrane staining with low background, demonstrating reliable performance.

Excellent, submitted by on
SKU PB9234
Application Immunofluorescence
Sample human Caco-2 cell line
Sample Processing Description Cells were normally cultured in 24-well plates using MEM supplemented with 20% fetal bovine serum. When the cell density reached approximately 60%, the culture was terminated. The medium was removed, cells were washed three times with PBS, fixed with 4% paraformaldehyde for 15 minutes, and then washed three times with PBS before further use.
Other ReagentsGoat serum, DAPI, and an anti-fade mounting medium.
Primary Antibody ZO1 tight junction protein/TJP1 Antibody Picoband®
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro488 Conjugated
Secondary Incubation 45 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary Caco-2 cells are a “gold standard” in vitro model for studying intestinal epithelial function, and ZO-1 is a key marker of barrier integrity. The images show immunofluorescence staining of ZO-1 in normally cultured Caco-2 cells to evaluate antibody performance. Clear membrane localization and accurate staining indicate that this antibody is suitable for downstream research applications.

Immunofluorescence using Anti-GFAP antibody (MA1045) clearly labeled protoplasmic astrocytes in the spinal cord gray matter, showing dense, bushy processes surrounding neurons, with excellent specificity and staining.

Excellent, submitted by on
SKU MA1045
Application Immunofluorescence
Sample rat spinal tissue
Sample Processing Description Paraffin-embedded transverse sections of rat spinal cord were prepared after formalin fixation.
Other ReagentsTris-EDTA Antigen Retrieval Buffer (50×, pH 9.0), DAPI
Primary Antibody GFAP Antibody (Monoclonal, G-A-5)
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Goat Anti-Mouse IgG (H+L) Secondary Antibody, Fluoro488 Conjugated
Secondary Incubation 45 minutes in 37℃
Detection Imaging system:Leica DM2500
Results Summary GFAP is a marker of astrocytes. In this experiment, immunostaining for GFAP was used to label astrocytes in the gray matter of the spinal cord to observe their distribution, density, and morphology. The results showed that the labeled protoplasmic astrocytes in the gray matter had short, thick, and highly branched processes with rough surfaces, forming a dense “bushy” network tightly surrounding neuronal cell bodies and synapses, consistent with theoretical expectations and demonstrating excellent staining.