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In this Western blot experiment using Anti-AQP1 antibody (Cat# BM5035) on MADB106 rat mammary carcinoma cells, AQP1 protein levels were markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor.

Excellent, submitted by Xinshuo Wang, Xi’an Jiaotong University on 2026-02-25

SKU	M00865
Application	Western blot
Sample	MADB106 rat mammary carcinoma cells
Sample Processing Description	MADB106 cells under normal culture conditions , MADB106 cells treated with agonist , MADB106 cells treated with inhibitor
Other Reagents	RIPA Lysis Buffer, Protease Inhibitor, Resolving Gel Solution ,Transfer Buffer ,Blocking Buffer
Primary Antibody	AQP1 Rabbit Monoclonal Antibody
Primary Incubation	1:3000, overnight at 4 ℃
Secondary Antibody	1:10,000, HRP-conjugated Goat Anti-Rabbit IgG
Secondary Incubation	1h at RT
Detection	Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary	AQP1 (Aquaporin 1) is the first discovered water channel protein, whose primary function is efficient water transport. Recent studies have revealed that AQP1 plays a critical role in cancer progression, promoting tumor growth in breast cancer by enhancing angiogenesis and cell migration. In this experiment, AQP1 protein levels were markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor.

The Anti-c-FOS antibody (M00297) showed clear and specific immunofluorescence staining in mouse brain, with markedly increased c-Fos expression in neurons of the acute ischemic region compared to normal brain.

Excellent, submitted by Qihang Yang, Huazhong University of Science and Technology on 2026-02-12

SKU	M00297
Application	Immunocytochemistry
Sample	mouse brain
Sample Processing Description	Normal brain tissue and brain tissue from a cerebral infarction model
Other Reagents	Goat serum, DAB Chromogenic Solution, Anti-fade mounting medium
Primary Antibody	c-Fos Rabbit Monoclonal Antibody
Primary Incubation	1:200, overnight at 4 ℃
Secondary Antibody	DyLight 594 Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	45 min at 37℃
Detection	Imaging system:Leica DM2500
Results Summary	c-Fos is a marker of activated neural cells, especially neurons. During the acute phase of cerebral infarction, as a strong stress and injury stimulus, it drives c-Fos expression in the brain—particularly in the peri-infarct region—far higher than in normal resting brain. The experimental results show clear staining of positive cells, and the expression levels are consistent with expectations.

The Anti-FAP antibody (M00422) showed clear and specific IHC staining in human hepatocellular carcinoma, with low expression in adjacent normal tissue and strong expression in tumor stroma.

Excellent, submitted by Fei Long, Zhongnan Hospital of Wuhan University on 2026-02-12

SKU	M00422
Application	Immunohistochemistry
Sample	human liver cancer
Sample Processing Description	Collected clinical surgical human hepatocellular carcinoma and adjacent peritumoral tissues were fixed in formalin and embedded in paraffin.
Other Reagents	Goat serum, DAB Chromogenic Solution
Primary Antibody	FAP1 Rabbit Monoclonal Antibody
Primary Incubation	1:200, overnight at 4 ℃
Secondary Antibody	Two-step IHC kit (Immunohistochemistry kit
Secondary Incubation	30 min at 37℃
Detection	Imaging system:Leica DM2500
Results Summary	FAP (fibroblast activation protein) expression is mainly associated with stromal cells in the tumor microenvironment and is very low in normal human tissues. Its role in hepatocellular carcinoma (HCC) is complex, but overall it functions as an important pro-tumor factor. High FAP expression is associated with poor prognosis. It can remodel the extracellular matrix, promote tumor invasion and metastasis, and suppress anti-tumor immunity. In this study, FAP showed low expression in peritumoral tissues (close to normal tissue) and high expression in the tumor stroma of HCC.

The Anti-TH antibody (M01917) demonstrated excellent specificity and clear fluorescence staining in IF analysis of paraffin-embedded mouse striatum sections, accurately labeling dopaminergic neurons with minimal background.

Excellent, submitted by Zengting Li, Huazhong University of Science and Technology on 2026-02-12

SKU	M01917
Application	Immunofluorescence
Sample	mouse brain
Sample Processing Description	Sagittal sections of mouse striatum were fixed in formaldehyde for 48 hours and paraffin-embedded.
Other Reagents	Goat serum，DAPI，Anti-fade mounting medium
Primary Antibody	Iba1 Rabbit Monoclonal Antibody
Primary Incubation	1:200, overnight at 4 ℃
Secondary Antibody	1:500, Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro594 Conjugated
Secondary Incubation	45 min at 37℃
Detection	Imaging system:Leica DM2500
Results Summary	TH is a marker of catecholaminergic neurons and can specifically label and identify dopaminergic, noradrenergic, and adrenergic neurons. In this experiment, TH immunofluorescence was performed to observe the distribution of dopaminergic neurons in the striatum. The results show clear staining and precise localization.

Produced clear, sharp bands with no background. Excellent specificity and consistency across replicates. Ideal as a loading control for WB.

Excellent, submitted by David Lee on 2026-01-08

SKU	A01263
Application	Western Blot
Sample	Human 293T and A549 cell lysates, Mouse brain tissue
Sample Processing Description	Cells were lysed in RIPA buffer containing protease inhibitors. Lysates were clarified by centrifugation and quantified using a BCA assay. 30–35 µg of protein was loaded per lane on a 5–20% SDS-PAGE gel and transferred to nitrocellulose membranes.
Primary Antibody	Anti-beta Actin ACTB Antibody
Primary Incubation	1:1000, incubated overnight at 4°C.
Secondary Antibody	Goat anti-rabbit IgG-HRP (Catalog # BA1054).
Secondary Incubation	1:5000 dilution, 1 hour at room temperature.
Other Reagents used	5% non-fat milk/TBST blocking buffer, ECL Plus substrate, Azure Biosystems c600 imaging system.
Detection	Chemiluminescent detection. Strong single band observed at ~42 kDa, corresponding to beta actin.
Results Summary	Produced clear, sharp bands with no background. Excellent specificity and consistency across replicates. Ideal as a loading control for WB.

This antibody is highly specific and efficient, suitable for detecting STAT3 protein in rat colon by Western blot, with clear results.

Excellent, submitted by Shiyu Zhang, LUTCM on 2026-01-08

SKU	M00007
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents	Blocking buffer
Primary Antibody	Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1:5000, 1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The figure shows Western blot results of the target protein STAT3 and the loading control Actin in the colon of normal rats, model rats, low/medium/high dose Chinese medicine treatment groups, and Western medicine treatment group. No differences were observed between the groups. The target bands are clear and distinct, and the experimental results are satisfactory.

This antibody is highly efficient and specific, suitable for Western blot detection of STAT3 (Phospho-Y705) protein in rat colon tissue, with only minor nonspecific bands observed.

Excellent, submitted by Shiyu Zhang, LUTCM on 2026-01-07

SKU	P00007-2
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents	Blocking buffer
Primary Antibody	Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1:5000, 1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The figure shows representative Western blot results of the target protein STAT3 (Phospho-Y705) and the internal control Actin in rat colon tissue from the normal control group, disease model group, low-, medium-, and high-dose traditional Chinese medicine–treated groups, and the western medicine–treated group. The target bands are clear and well defined, and the experimental results are satisfactory.

This antibody is highly specific and efficient, suitable for detecting RHOA/B/C protein in rat colon by Western blot, with only minimal non-specific bands.

Excellent, submitted by Shiyu Zhang, LUTCM on 2026-01-06

SKU	M00207-1
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents	Blocking buffer
Primary Antibody	Rho A + B + C Rabbit Monoclonal Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1:5000, 1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The figure shows the Western blot results of the target protein RHOA/B/C and the internal control Actin in rat colon tissue across the following groups: normal, disease model, low/middle/high dose traditional Chinese medicine treatment, and Western medicine treatment. The target bands are clear and distinct, and the experimental results are satisfactory.

This antibody is highly efficient and specific, suitable for Western blot detection of PTEN protein in rat colon tissue, with only minor nonspecific bands.

Excellent, submitted by Shiyu Zhang, LUTCM on 2026-01-06

SKU	M00006-2
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents	Blocking buffer
Primary Antibody	PTEN Rabbit Monoclonal Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1:5000, 1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The figure shows a schematic of Western blot results for the target protein PTEN and the internal control Actin in rat colon across different groups. Expression was increased in the model group. Among the low, medium, and high doses of the herbal treatment, the low-dose group showed the best effect. The target bands are clear, and the experimental results are satisfactory.

This antibody exhibits high efficiency and specificity and is suitable for Western blot detection of CCND1 protein in rat colon tissue, with only minor nonspecific bands observed.

Excellent, submitted by Shiyu Zhang, LUTCM on 2026-01-06

SKU	M00149-1
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents	Blocking buffer
Primary Antibody	Cyclin D1 CCND1 Rabbit Monoclonal Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The figure shows the Western blot results of the target protein CCND1 and the internal control Actin in rat colon tissue across the following groups: normal, disease model, low/middle/high dose traditional Chinese medicine treatment, and Western medicine treatment. The target bands are clear and distinct, and the experimental results are satisfactory.