• Compare Products

  • Table of Contents

Customer Testimonials

Peer Reviews from Real Labs

Browse peer-submitted results linked to specific products—ratings, images, and practical notes included. Contribute your own result to strengthen the evidence base and claim rewards after verification.

Share Your Review Browse Reviews ↓

Video Interviews

See real workflows and decision points—what researchers tried, what worked, and why. Share your story in a short interview and claim rewards!

Get Interview Rewards

Filter by category, reactivity, and host to find the most relevant peer results.

The AKT1 Antibody (M00024-1) showed clear, specific bands in WB of rat skeletal muscle, with expression changes consistent with expectations.

Excellent, submitted by on
SKU M00024-1
Application Western Blot
Sample Rat skeletal muscle tissue
Sample Processing Description Normal rat skeletal muscle, injury model, and high-, medium-, and low-dose treatment groups, with total protein extracted.
Other Reagents RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody AKT1 Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:10000, 1 h in RT
Detection Substrate: ECL substrate, Image system: ChemiDoc MP
Results Summary The AKT1 bands are clear and correctly positioned; expression is significantly higher in the model group than in the control, and decreases with increasing drug dose in the treatment groups, consistent with expectations.

MTOR Antibody (M00003) WB on rat skeletal muscle shows clear bands, with higher MTOR in the model and dose-dependent reduction in treatment groups.

Excellent, submitted by on
SKU M00003
Application Western Blot
Sample Rat skeletal muscle tissue
Sample Processing Description Total protein was extracted from rat skeletal muscle tissue: ① normal, ② injury model, ③ high-dose drug treatment, ④ medium-dose drug treatment, ⑤ low-dose drug treatment.
Other ReagentsRIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody mTOR/Tor Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:10000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary The results show clear MTOR bands, with higher levels in the injury model compared to normal, and a dose-dependent decrease in the treatment groups, as expected.

WB of COL1A1 (Cat# PA2140-1) in mouse cardiac tissues from 3-, 6-, 12-, and 18-month-old mice shows that COL1A1 levels clearly increase with age.

Excellent, submitted by on
SKU PA2140-1
Application Western Blot
Sample mouse cardiac tissue
Sample Processing Description Total protein was extracted from mouse cardiac tissues at 3, 6, 12, and 18 months of age.
Other Reagents RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody Collagen I/COL1A1 Antibody Picoband®
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:10000, 1 h in RT
Detection Substrate: ECL substrate, Image system: ChemiDoc MP
Results Summary This experiment aimed to investigate changes in COL1A1 expression in mouse cardiac tissues from 3-, 6-, 12-, and 18-month-old mice (representing young, middle-aged, middle-old, and old stages), and the results show that COL1A1 levels clearly increase with age.

The Anti-α-SMA antibody (Cat# BM3902) shows clear and specific bands with low background in WB analysis of mouse heart tissues across different ages, with results consistent with expected trends.

Excellent, submitted by on
SKU M01072-1
Application Western Blot
Sample mouse cardiac tissue
Sample Processing Description Mouse cardiac tissues were collected from 3-, 6-, 12-, and 18-month-old mice, and total protein was extracted.
Other Reagents RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:10000, 1 h at RT
Detection Substrate: ECL substrate
Imaging system: ChemiDoc MP
Results Summary This experiment aimed to investigate the changes in α-SMA expression in mouse cardiac tissues from 3-, 6-, 12-, and 18-month-old mice (representing young, middle-aged, and aged stages). The results show that α-SMA levels are generally similar across different ages, with a slight but not significant increase as age progresses.

Western blot using Anti-ER/ESR1 Antibody (M00057-2) in mouse brain tissues showed clear, specific ESR1 bands across normal, disease model, and AB drug-treated groups, with β‑actin as a consistent loading control.

Excellent, submitted by on
SKU M00057-2
Application Western Blot
Sample mouse brain tissue
Sample Processing Description Mouse brain tissues were lysed in RIPA buffer containing a protease inhibitor cocktail at 4°C for 2 hours, centrifuged to collect the supernatant, and protein concentration was determined. After adjusting the concentration, samples were mixed with 5× protein loading buffer, denatured by heating at 95–100°C for 10 minutes, and 15 μL of protein was loaded per lane for SDS-PAGE.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody goat anti rabbit secondary antibodies
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary This antibody is highly sensitive, produces clear WB bands, is reusable, offers excellent cost-effectiveness, and demonstrates a clear advantage over similar international products, making it highly recommended for use.

WB results showed that Cingulin/CGN (A02373-1) was downregulated in the rat colon model group and restored after Chinese medicine treatment, with the high-dose group showing the best effect, and the target bands were clear and specific.

Excellent, submitted by on
SKU A02373-1
Application Western Blot
Sample rat colon tissue
Sample Processing Description Rat colon tissues were lysed in RIPA buffer containing PMSF (100:1) for 10 min, centrifuged at 12,000 rpm for 15 min, and the supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 min, and then loaded onto SDS-PAGE.
Other Reagents5% non-fat milk
Primary Antibody Cingulin/CGN Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary The figure shows WB results of Cingulin/CGN and the internal control Actin in rat colon across different groups; expression was decreased in the model group and restored after Chinese medicine treatment, with the high-dose group showing the best effect, and the target bands were clear and specific, indicating satisfactory results.

Anti-PRLR Antibody (PA2087) demonstrated clear and specific detection of PRLR in mouse brain tissue by Western blot, with distinct differences observed among the control, model, and AB treatment groups.

Excellent, submitted by on
SKU PA2087
Application Western Blot
Sample mouse brain tissue
Sample Processing Description Mouse brain tissues were lysed in RIPA buffer containing a protease inhibitor cocktail at 4 °C for 2 hours. After centrifugation, the supernatant was collected for protein quantification. The protein concentration was adjusted accordingly, mixed with 5× protein loading buffer, and denatured by heating for 10 minutes. Then, 15 μl of protein sample was loaded per lane for electrophoresis.
Other Reagents blocking buffer
Primary Antibody Prolactin Receptor/PRLR Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:2000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary The figure shows representative Western blot results of PRLR and the internal control β-actin in brain tissues from normal mice, the model group, and mice treated with low and high doses of AB. The antibody produced clear bands, and distinct differences among the experimental groups were clearly observed.

Using Boster’s IL-6 antibody (RP1012) in WB on HK-2 cells produced clear, specific bands with high sensitivity and cost-effectiveness, outperforming previously tested antibodies.

Excellent, submitted by on
SKU RP1012
Application Western Blot
Sample human HK-2 cells
Sample Processing Description Cell samples were directly lysed in RIPA buffer, mixed with loading buffer at the appropriate ratio, and denatured at 98 °C. Twenty microliters of each protein sample were loaded per lane onto SDS-PAGE.
Other Reagents 5% non-fat milk
Primary Antibody Interleukin-6 IL6 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L)
Secondary Incubation 1 h in RT
Detection Substrate: ECL substrate
Results Summary Previously, WB experiments were performed using antibodies from two domestic and international suppliers, which showed poor specificity and were expensive. Subsequently, Boster’s IL-6 antibody (Cat. RP1012) was used, demonstrating high specificity, strong titer, cost-effectiveness, and yielding clear bands.

Pancreatic IL-6 levels measured using the Boster Anti-IL-6 antibody (PB9034) demonstrated high specificity and sensitivity in WB analysis, providing reliable evidence for evaluating the anti-inflammatory effect of the nanodrug.

Excellent, submitted by on
SKU PB9034
Application Western Blot
Sample mouse PACs cells
Sample Processing Description After centrifugation, the supernatant was collected. A small aliquot of the protein solution was taken for BCA quantification, and the remaining protein solution was mixed with an equal volume of loading buffer, then denatured in a 100 °C water bath for 5 minutes.
Other Reagents Protein-Free Rapid Blocking Buffer
Primary Antibody Anti-IL6 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L)
Secondary Incubation 1 h in RT
Detection Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary The levels of IL-6 and IL-1β in the pancreas are key indicators for evaluating whether the nanodrug exerts anti-inflammatory effects. Initially, antibodies from two domestic and international suppliers were used for WB experiments, but their specificity was relatively poor. Subsequently, the antibody from Boster was adopted, which demonstrated strong specificity, high titer, and cost-effectiveness.

In WB using Anti-CDK1 (Phospho-T450) antibody (Cat# PB9533-50 µL), CDK1 expression in rat colon was increased in the model group and most effectively reduced in the high-dose herbal treatment group, with clear and well-defined target bands.

Excellent, submitted by on
SKU PB9533
Application Western Blot
Sample rat colon tissue
Sample Processing Description Samples were lysed in RIPA buffer containing PMSF protease inhibitor (100:1) for 10 min, centrifuged at 12,000 rpm for 15 min, and the supernatant was mixed with 5× loading buffer, boiled at 100 °C for 10 min, and loaded onto SDS-PAGE.
Other Reagents 5% Non-fat milk
Primary Antibody CDK1 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The image shows WB results of CDK1 and the loading control Actin in rat colon across different groups; CDK1 expression was elevated in the model group and most effectively reduced in the high-dose herbal treatment group, with clear and distinct target bands.