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A strong nuclear signal for Cyclin E was observed in proliferating cells by IF. Results were reproducible and matched the expected patterns.

Excellent, submitted by on
SKU DZ41310
Application Immunofluorescence
Sample Human HaCaT cell
Sample Processing Description Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes and blocked in 1% BSA for 1 hour before incubation with the primary antibody.
Primary Antibody Human CCNE1 Antibody
Primary Incubation 1:100-1:200, overnight at 4 ℃
Secondary Antibody Goat anti-rabbit IgG conjugated to Alexa Fluor 488
Secondary Incubation 1:1000, 1-2 hours in room temperature
Other Reagents used PBS, 0.1% Triton X-100, 1% BSA, DAPI for nuclear counterstaining
Detection Fluorescence microscopy using AlexaFluor488 (excitation 488 nm, emission 519 nm) using Zeiss LSM 900 Confocal Microscope with Airyscan 2
Results Summary The CyclinE-N15 antibody (DZ41310) worked fine in IF application using human cell lines. In IF, the nuclear localization of Cyclin E matched the known expression pattern. A fluorescent secondary antibody (Alexa Fluor 488) was used for detection and gave clear signal. Overall, a reliable antibody for D15 transcript Cyclin E detection in human cell systems.

Using freshly prepared blocking buffer helped reduce background. Results were reproducible and matched the expected patterns.

Excellent, submitted by on
SKU DZ41310
Application Western Blot
Sample Human HaCaT cell, Human A2780 cell, Human HCT cell
Sample Processing Description Dissection, Homogenization, Sample boiling in 1X Lamelli, SDS-PAGE, Standard Western Blotting
Primary Antibody Human CCNE1 Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody Anti-rabbit/mouse IgG horseradish peroxidase-conjugated
Secondary Incubation 1:10000, 1-2 hours in room temperature
Detection Biorad Chemidoc
Results Summary Using freshly prepared blocking buffer helped reduce background. Results were reproducible and matched the expected patterns.

The antibody performs efficiently and specifically, with very few nonspecific bands.

Excellent, submitted by on
WB result image
SKU A03213-2
Application Western Blot
Sample MCF-7 cell
Sample Processing Description Cells were directly lysed in NP-40 buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98 °C. Then, 20 µL of protein sample was loaded per lane onto SDS-PAGE.
Primary Antibody UBA6 Antibody
Primary Incubation 1:1000, overnight at 4 °C
Blocking Agent 5% Non-fat milk
Secondary Antibody HRP-conjugated Goat Anti-Rabbit IgG
Secondary Incubation Incubate at room temperature for 1 hour
Detection Signal was developed using ECL substrate on a Tanon system.
Results Summary The antibody performs efficiently and specifically, with very few nonspecific bands.

Works very well in Western blot for rat enteric glial cells and mouse FGL-2 protein, with only slight nonspecific bands.

Excellent, submitted by on
SKU A06303-3
Application Western Blot
Sample rat enteric glial cells and mouse tissue
Sample Processing Description Samples (5 µL per lane) were run on 10% resolving and stacking gels at 80 V for 20 min, then 150 V for 70 min, and transferred at 400 mA for 100 min.
Primary Incubation The membrane was incubated with the FGL-2 primary antibody (1:1000) overnight at 4 °C.
Secondary Antibody HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody
Secondary Incubation Incubate at room temperature for 2 hour
Other Reagents used 5% non-fat milk
Detection Signal was developed using ECL substrate on an ImageQuant LAS4000mini system.
Results Summary Works very well in Western blot for rat enteric glial cells and mouse FGL-2 protein, with only slight nonspecific bands.

High-Quality Serinc5 Antibody From Boster Bio Produces a Clear Band

Excellent, submitted by on

Source: Biocompare.com

SKU A06603
Application Western Blot
Sample Ripa buffer lysis transfected HEK293 cell
Primary Incubation +4°C overnight
Blocking Agent 5% milk in 1X TBST
Secondary Incubation 30 mins
Tertiary Incubation 1:1000
Detection Clarity Western ECL Substrate
Results Summary The antibody is excellent at recognizing and expressing the protein at the correct position. I have tried samples from different vendors, but only this vendor offers high-quality products.

Mettl23 Detection in Cell Lines

Excellent, submitted by on

Source: Biocompare.com

SKU DZ41142
Application Western Blot
Sample Human cell lines
Primary Incubation 1:500 with 1% BSA in TBS, incubated overnight, at 4oC, with shaking
Blocking Agent 5% BSA in TBS
Secondary Incubation Licor IRDye 800 Goat anti Rabbit diluted 1:10000 with 1% BSA in TBS, incubated for 1 hour at RT , with shaking
Detection Licor Odyssey IR Scanner
Additional Notes The antibody has very good stability at room temperature (transit took over 2 weeks and the ice packs had long melted before delivery).
Results Summary The antibody gave a detectable band at the expected size for all cell lines tested with no non-specific bands.

"This antibody was used to determine the level of protein expression in various cell lines in order to set up two groups (high vs low expression). This antibody was custom made because numerous suppliers have either stopped their production for this antibody or quality/specificity was low."