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In the IF experiment using Anti-IBA-1 antibody (Cat# M01394-4), the antibody clearly and specifically labeled microglial cells in mouse cerebral infarction tissue, showing distinct staining and well-defined cellular morphology.

Excellent, submitted by on
SKU M01394-4
Application Immunofluorescence
Sample mouse brain tissue
Sample Processing Description Mouse cerebral infarction model; brain tissues were collected, fixed in formaldehyde for 48 hours, and then sagittally paraffin-embedded.
Other ReagentsGoat serum, DAPI Staining Solution, Antifade fluorescence mounting medium.
Primary Antibody Iba1 Rabbit Monoclonal Antibody
Primary Incubation 1:100, overnight at 4 ℃
Secondary Antibody Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro594 Conjugated (BA1142, Boster)
Secondary Incubation 45 min at 37℃
Detection Imaging system:Leica DMi3000
Results Summary IBA-1 is a well-established marker of microglia in the nervous system. Microglia are the primary immune effector cells in the central nervous system and respond rapidly to CNS injury by proliferating, upregulating or re-expressing MHC antigens, migrating, and adopting a phagocyte-like morphology. In this study, IBA-1 was used to label microglia in cerebral infarction samples, showing clear staining and accurate cellular morphology.

In this IHC experiment using Anti-CD4 antibody (Cat# M00344) on human cervical cancer paraffin sections, CD4-positive helper T cells were clearly and specifically stained with minimal background, demonstrating excellent antibody performance.

Excellent, submitted by on
SKU M00344
Application Western blot
Sample human cervical cancer paraffin sections
Sample Processing Description Cervical cancer tissue collected from clinical surgery, fixed in formalin and paraffin-embedded
Other ReagentsGoat serum, DAB substrate solution
Primary Antibody CD4 Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Two-step IHC detection kit
Secondary Incubation 30 min at 37℃
Detection Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary CD4 is a marker of helper T cells; in this IHC experiment, CD4 staining clearly identified helper T cells in human cervical cancer samples, showing excellent specificity and performance of the antibody.

In this Western blot experiment using Anti-AQP1 antibody (Cat# BM5035) on MADB106 rat mammary carcinoma cells, AQP1 protein levels were markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor.

Excellent, submitted by on
SKU M00865
Application Western blot
Sample MADB106 rat mammary carcinoma cells
Sample Processing Description MADB106 cells under normal culture conditions , MADB106 cells treated with agonist , MADB106 cells treated with inhibitor
Other ReagentsRIPA Lysis Buffer, Protease Inhibitor, Resolving Gel Solution ,Transfer Buffer ,Blocking Buffer
Primary Antibody AQP1 Rabbit Monoclonal Antibody
Primary Incubation 1:3000, overnight at 4 ℃
Secondary Antibody 1:10,000, HRP-conjugated Goat Anti-Rabbit IgG
Secondary Incubation 1h at RT
Detection Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary AQP1 (Aquaporin 1) is the first discovered water channel protein, whose primary function is efficient water transport. Recent studies have revealed that AQP1 plays a critical role in cancer progression, promoting tumor growth in breast cancer by enhancing angiogenesis and cell migration. In this experiment, AQP1 protein levels were markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor.

The Anti-c-FOS antibody (M00297) showed clear and specific immunofluorescence staining in mouse brain, with markedly increased c-Fos expression in neurons of the acute ischemic region compared to normal brain.

Excellent, submitted by on
SKU M00297
Application Immunocytochemistry
Sample mouse brain
Sample Processing Description Normal brain tissue and brain tissue from a cerebral infarction model
Other ReagentsGoat serum, DAB Chromogenic Solution, Anti-fade mounting medium
Primary Antibody c-Fos Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody DyLight 594 Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 45 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary c-Fos is a marker of activated neural cells, especially neurons. During the acute phase of cerebral infarction, as a strong stress and injury stimulus, it drives c-Fos expression in the brain—particularly in the peri-infarct region—far higher than in normal resting brain. The experimental results show clear staining of positive cells, and the expression levels are consistent with expectations.

The Anti-FAP antibody (M00422) showed clear and specific IHC staining in human hepatocellular carcinoma, with low expression in adjacent normal tissue and strong expression in tumor stroma.

Excellent, submitted by on
SKU M00422
Application Immunohistochemistry
Sample human liver cancer
Sample Processing Description Collected clinical surgical human hepatocellular carcinoma and adjacent peritumoral tissues were fixed in formalin and embedded in paraffin.
Other ReagentsGoat serum, DAB Chromogenic Solution
Primary Antibody FAP1 Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Two-step IHC kit (Immunohistochemistry kit
Secondary Incubation 30 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary FAP (fibroblast activation protein) expression is mainly associated with stromal cells in the tumor microenvironment and is very low in normal human tissues. Its role in hepatocellular carcinoma (HCC) is complex, but overall it functions as an important pro-tumor factor. High FAP expression is associated with poor prognosis. It can remodel the extracellular matrix, promote tumor invasion and metastasis, and suppress anti-tumor immunity. In this study, FAP showed low expression in peritumoral tissues (close to normal tissue) and high expression in the tumor stroma of HCC.

The Anti-TH antibody (M01917) demonstrated excellent specificity and clear fluorescence staining in IF analysis of paraffin-embedded mouse striatum sections, accurately labeling dopaminergic neurons with minimal background.

Excellent, submitted by on
SKU M01917
Application Immunofluorescence
Sample mouse brain
Sample Processing Description Sagittal sections of mouse striatum were fixed in formaldehyde for 48 hours and paraffin-embedded.
Other ReagentsGoat serum，DAPI，Anti-fade mounting medium
Primary Antibody Iba1 Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody 1:500, Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro594 Conjugated
Secondary Incubation 45 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary TH is a marker of catecholaminergic neurons and can specifically label and identify dopaminergic, noradrenergic, and adrenergic neurons. In this experiment, TH immunofluorescence was performed to observe the distribution of dopaminergic neurons in the striatum. The results show clear staining and precise localization.

One major band was detected at the expected size (84 kDa), while multiple minor bands were detected in the egg lysate under the condition tested.

Excellent, submitted by on
SKU DZ41739
Application Western Blot
Sample egg lysate
Sample Processing Description Egg lysate was prepared and loaded onto a 10% polyacrylamide gel for electrophoresis.
Primary Antibody Anti-Purple sea urchin LOC581356 Antibody
Primary Incubation 1:2000, incubated overnight at 4°C.
Secondary Antibody Goat anti-rabbit IgG-HRP
Secondary Incubation 2 hour at room temperature.
Detection Chemiluminescent detection.
Results Summary One major band was detected at the expected size (84 kDa), while multiple minor bands were detected in the egg lysate under the condition tested.

A single band was detected at the expected size of 60kDa in the gastrula stage lysate.

Excellent, submitted by on
SKU DZ41738
Application Western Blot
Sample Embryo lysate
Sample Processing Description Embryonic lysate was prepared and loaded onto a 10% polyacrylamide gel for electrophoresis.
Primary Antibody Anti-Purple sea urchin LOC584590 Antibody
Primary Incubation 1:2000, incubated overnight at 4°C.
Secondary Antibody Goat anti-rabbit IgG-HRP
Secondary Incubation 2 hour at room temperature.
Detection Chemiluminescent detection.
Results Summary A single band was detected at the expected size of 60kDa in the gastrula stage lysate.

An shRNA targeting Atxn7 displayed a decrease in Atxn7 abondance showing specificity of the antibody.

Excellent, submitted by on
SKU DZ41648
Application Western Blot
Sample 3T3 Cell lysate
Sample Processing Description Nuclear and Cytoplasmic Extraction was performed on 3T3 cells with NE-PER kit by following the protocol and by adding protease and phosphatase inhibitors. Total protein was quantified with Qubit Protein Assay. Lysates were mixed with 4× Laemmli Sample Buffer and 2-mercaptoethanol, and then heated at 95°C for 5 min.
Primary Antibody Anti-Mouse Atxn7 Antibody
Primary Incubation Incubated in non-fat dry milk in TBST overnight at 4°C with antibody dilution at 1:1000 dilution
Secondary Antibody Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
Secondary Incubation 1:4000 dilution in milk for 1 hour at room temperature
Other Reagents used SuperSignal West Pico PLUS Chemiluminescent Substrate
Detection Biorad ChemiDoc
Results Summary I was looking to target Atxn7 isoform 2, named as Atxn7b in the literature, and referenced as ENSMUST00000223714.2 on Ensembl. (It differs only on the C-terminus compared to Atxn7a, isoform 1). Atxn7 is mainly in the nucleus and is between 100-130 kDa. An shRNA targeting Atxn7 displayed a decrease in Atxn7 abondance showing specificity of the antibody.

This antibody is highly specific and efficient, suitable for detecting STAT3 protein in rat colon by Western blot, with clear results.

Excellent, submitted by on
SKU M00007
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows Western blot results of the target protein STAT3 and the loading control Actin in the colon of normal rats, model rats, low/medium/high dose Chinese medicine treatment groups, and Western medicine treatment group. No differences were observed between the groups. The target bands are clear and distinct, and the experimental results are satisfactory.