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User Friendly And Economical ELISA Kit

--Dhruva Bhattacharya, University of Arizona, Opthalmology and Vision Science, Postdoctoral Research Associate

Excellent, submitted by on

Source: Biocompare.com

SKU EK0392
Application ELISA
Sample "Culture media supernatant" of transformed cultured cells of human conjunctiva epithelial cells
Detection --

"I am very much satisfied with the product Human IL-1 Beta PicoKine™ ELISA Kit (EK0392) purchased from Boster Bio. I used the Boster Bio ElISA kit for the first time and I am very happy with this product. After trying the IL-1beta kit, I have purchased two other cytokine detection ELISA kits from Boster Bio. The methodology of Human IL-1 Beta PicoKine™ ELISA Kit is simplified and user friendly. It required minimum optimization for my experiments. The written instructions provided with the kit is very useful for running the ELISA. The results obtained so far have been consistent and reproducible. For my future ELISA experiments, I'll be buying the ELISA kit from Boster Bio."

Very Easy To Work With And Great Results With Least Standard Error

--Akshay Jain, University of Missouri, Kansas City, Pharmaceutical Sciences, Research Assistant

Excellent, submitted by on

Source: Biocompare.com

SKU EK0373
Applications ELISA (Inflammatory cytokine assay from plasma)
Sample Rat plasma
Detection --

"I used this assay kit for detection of the cytokines in the plasma samples. Only 100 uL of sample yielded absolutely flawless results. Plasma samples showed variable cytokine expression among independent groups which demonstrates the accuracy of this kit. Straightforward protocol. Very easy to perform. Highly recommended product for precise cytokine assay analysis." (Customer’s Tip: "Keep working area clean. Prepare necessary dilution of the sample before starting the procedure.")

Human ADAMTS1 PicoKine ™ ELISA Kit Worked Well

--Huiyuan Tang, University of Utah, Hematology and Hematologic Malignancies, Lab Specialist

Excellent, submitted by on

Source: Biocompare.com

SKU EK1340
Applications ELISA
Sample Cell lysates from HUVEC treated with control siRNA or ADAMTS-13 siRNA
Detection --

"I used the kit to test the ADAMTS1 protein level in human endothelial cell lysates. The instructions are clear and the kit worked the first time with reproducible results. Decent expression level was detected and results are reproducible. Cheaper than most comparable product. I will keep using this product and will be happy to recommend to my colleagues." (Customer’s Tip: "Just follow the protocol.")

Works Well For Detection Of Gal-1 Over A Broad Range Of Concentration

--Mahesh Raundhal, Weill Cornell Medicine, Medicine, Postdoctoral Fellow

Excellent, submitted by on

Source: Biocompare.com

SKU EK0762
Applications ELISA
Sample Human ovarian cancer ascites
Detection --

"Human ovarian cancer ascites samples were tested for presence of Galectin-1 protein. Depending on the donor, ascites samples were found to have varying concentrations of Gal-1. Straightforward and easy to use once a general idea of Gal-1 concentration in sample of interest is optimized." (Customer’s Tip: "If using for the first time, plate samples at least 3-4 dilutions to get consistent results among samples at the same dilution.")

Good quality budget option

--Milos Lazic, JECURE THERAPEUTICS, SCIENTIST II

Excellent, submitted by on

Source: Biocompare.com

SKU EK0392
Applications ELISA
Sample protein concentrations in cell culture media
Detection --

"We are a pharmaceutical startup optimizing cell based drug assays where one of our endpoints was observing the differences in mature IL-1b production in the media. I have compared several kits from the most expensive versions of R&D, over eBioscience/Affymetrix to a more budget Boster's kit. The aim was to decide what is the best price for the value and quality we are getting for reliable and replicable ELISA analyses. This assay was used to test the effect of increasing concentrations of a drug on IL-1b levels (dose response). The kit reported similar data obtained from the other two kits (R&D and eBiosciences), with a slightly larger standard deviations. Results replicated the findings of the previous kits and the assay had good sensitivity/ instructions on how to perform assay and analyze data. I would use this assay in an academic lab for a project where no more than 2-3 96-well plates will be used. It should be done carefully by a scientist that has previously performed ELISA assays. There are many steps to it. It is a good quality budget option to the other leading kits on the market. I would not recommend it for a larger drug screen assay where more than 5 ELISAs kits ought to be used."

Kit worked great

--Charles Stopford, UNIVERSITY OF NORTH CARLINA, MICROBIOLOGY AND IMMUNOLOGY, RESEARCH SPECIALIST

Excellent, submitted by on

Source: Biocompare.com

SKU EK0969
Applications ELISA
Sample Primary keratinocytes
Detection --

"This kit was very straightforward and easy to use. Followed the protocol given in the kit...Straight forward experiment, no tips needed. Kit worked great - positive control was clearly positive. Nothing bad about the kit, although our experimental results were negative. We would buy this kit or similar ELISA kits again from BosterBio."

Great kit

--- Unattributed Review* (Reviews submitted to our site where we are unable to verify the identity of the author are considered “unattributed reviews".)

Excellent, submitted by on

Source: Biocompare.com

SKU EK0782
Applications IL-1RA detection in rat serum
Sample Rat serum
Detection --

"I used this kit to detect a recombinant IL1RA in rat serum and it worked very well. The procedure was easy to follow and my standard curve was right on target. If needed we will order this kit and use it again. The kit was able to detect our rIL1RA but was less sensitive in detecting an altered formulation of the protein."

Competitively priced and worked

--Lu Yang, UCSD, CMMW, GRADUATE STUDENT

Excellent, submitted by on

Source: Biocompare.com

SKU EK1232
Applications Protein level screen
Sample Cell culture media from MCF7 cells
Detection --

"The TFF1 ELISA kit from Boster was competitively priced and worked with cell culture media taken from MCF7 cells, a human mammary carcinoma which is well known for overexpression of the TFF1 protein. The kit is very sensitive, detecting TFF1 protein at levels of 10pg/mL. The kit is saturated about 1mg/mL, so in my case, a 1 to 100 dilution of cell culture media was needed in order to be in the linear range of the assay. Good ELISA kit for human TFF1 protein. Worked as advertised."

The standards and ELISA kit worked great.

--Jason Somarelli, DUKE UNIVERSITY, MEDICINE, RESEARCH ASSOCIATE

Excellent, submitted by on

Source: Biocompare.com

SKU EK0397
Applications Detection of secreted IL-2 in media of Jurkat T cells
Sample Jurkat T cell media (RPMI, 10% FBS, 1% pen/strep)
Detection --

"I was using this kit to detect IL-2 secretion from Jurkat T-cells upon activation with PMA and PHA. I followed the protocol exactly. I had extremely low levels of IL-2 in my experiment, which were just above background. In this case, it was essential for me to use the standards and blanks to ensure that my samples were above background. All components come with the kit, unlike some other kits from other companies in which only some of the necessary components are provided. This is a great kit that is a bit expensive, but worth the cost for an easy-to-use and sensitive assay."