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Immunofluorescence for Anti-CG6353 Antibody

--Amin Ghabrial  Pathology and Cell Biology  Columbia University  Principal Investigator

Excellent, submitted by on

Source: Biocompare.com

"My lab is characterizing a mutant we named disjointed. The disjointed gene is also known as CG6353 or Archease. We have used this antibody for IF, and it appears to work well. It gives us a staining pattern for the endogenous protein that matches the pattern we see with an HA-tagged fusion protein detected by anti-HA staining."

SKU DZ33921-1
Application Immunofluorescence
Sample Drosophila larvae
Primary Incubation   1:100 at 4 degrees overnight
Blocking Agent 4% horse serum
Secondary Incubation   1:1000 anti-Rb-A488
Tertiary Incubation   N/A
Detection IF
Results Summary Antibody staining reveals subcellular localization of the protein. In salivary gland and trachea, localization was primarily nuclear.

Western blot for Polyclonal Anti-Drosophila melanogaster (Fruit fly) emp Antibody

--Yuzuru Imai, Department of Research for Parkinson's Disease Juntendo University Graduate School of Medicine

Excellent, submitted by on
SKU DZ41152
Application Western blot
Sample Drosophila melanogaster (head and thorax)
Detection Immobilon Forte Western HRP substrate

"There are non-specific bands and immunostaining may not be possible, but Western blotting is sufficient with this antibody."

Immunohistochemistry for Anti-Mannose Receptor/MRC1

Excellent, submitted by on
SKU A02285-2
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Mouse skin
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 37°C for 30 minutes
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

A Good Drosophila Me31B Antibody for WB

--Svetlana Dzitoyeva, University of Colorado, Denver, Biochemistry and Molecular Genetics, Research Scientist

Excellent, submitted by on

Source: Biocompare.com

SKU DZ33938-1
Application Western Blot
Sample Insect, D. melanogaster
Detection ECL

"The research of our lab aims to understand the mechanisms of maternally deposited RNAs and proteins clearance during maternal-to-zygotic transition (MZT). This is a very good AB, tested on WB only. The image shows GFP-tagged Me31B. Very good and clean. Clean and sharp band. Recommend Boster antibody production service."