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| SKU | DZ4103 |
|---|---|
| Application | Immunofluorescence |
| Sample | wildtype and KO mutant zebrafish retina |
| Primary Incubation | 1:100 |
| Blocking Agent | 10% normal goat serum and 2% bovine serum albumin in PBS |
| Secondary Antibody | Alexa Fluor 488 goat anti-rabbit and was used in a 1:800 dilution. |
| SKU | DZ41032 |
|---|---|
| Application | Immunofluorescence |
| Sample | wildtype and KO mutant zebrafish retina |
| Primary Incubation | 1:100 |
| Blocking Agent | 10% normal goat serum and 2% bovine serum albumin in PBS |
| Secondary Antibody | Alexa Fluor 568 goat anti-rabbit was used in a 1:800 dilution. |
Source: Biocompare.com
| SKU | DZ41383 |
|---|---|
| Application | Immunofluorescence |
| Sample | Zebrafish WKM, IPEX and FFPE tissue sections |
| Primary Incubation | Overnight |
| Blocking Agent | 3% goat serum in PBS |
| Secondary Incubation | 1 hour |
| Tertiary Incubation | None |
| Detection | Fluorescence microscopy |
| DOI or PMID # | https://doi.org/10.1101/2024.04.29.591640 |
| Results Summary | Antibody detects eosinophils and stains eosinophil granula. It is highly specific and can be used in dilutions such as 1:1000 for IF. |
"We ordered this antibody as a custom antibody from Boster Bio to stain for zebrafish eosinophils in FFPE tissue sections and fixed cells. It works wonderfully: very bright, with no relevant background or off-target staining, and highly reproducible."
Source: Customer Feedback Submission
--Jason Tennessen
| SKU | DZ41223 |
|---|---|
| Application | IF |
| Sample | Drosophila larval fat body |
"We’ve verified the specificity of this custom antibody that Boster generated for us. The antibody "Anti-Fruit Fly Gpdh1 Antibody (DZ41223)” works well for immunofluorescence. A 1:100 dilution of this antibody stains fat body tissues of wild-type larvae but not Gpdh1 mutant. I’m happy to advertise this antibody to the fly community."
Source: Biocompare.com
"My lab is characterizing a mutant we named disjointed. The disjointed gene is also known as CG6353 or Archease. We have used this antibody for IF, and it appears to work well. It gives us a staining pattern for the endogenous protein that matches the pattern we see with an HA-tagged fusion protein detected by anti-HA staining."
| SKU | DZ33921-1 |
|---|---|
| Application | Immunofluorescence |
| Sample | Drosophila larvae |
| Primary Incubation | 1:100 at 4 degrees overnight |
| Blocking Agent | 4% horse serum |
| Secondary Incubation | 1:1000 anti-Rb-A488 |
| Tertiary Incubation | N/A |
| Detection | IF |
| Results Summary | Antibody staining reveals subcellular localization of the protein. In salivary gland and trachea, localization was primarily nuclear. |
| SKU | DZ41152 |
|---|---|
| Application | Western blot |
| Sample | Drosophila melanogaster (head and thorax) |
| Detection | Immobilon Forte Western HRP substrate |
"There are non-specific bands and immunostaining may not be possible, but Western blotting is sufficient with this antibody."
| SKU | DZ01398-1 |
|---|---|
| Application | Immunofluorescence |
| Sample | Zebrafish heart |
| Detection | Confocal Microscopy |
"Mef2a was detected in a cryosection of zebrafish heart using the rabbit anti-Mef2a antibody (cat# DZ01398-1, 1:200, 4 degrees overnight). AlexaFluor546 Conjugated Goat Anti-Rabbit IgG was used as a secondary antibody at 1:200 dilution and incubated for 1.5 h at room temperature."
Source: Biocompare.com
| SKU | DZ33938-1 |
|---|---|
| Application | Western Blot |
| Sample | Insect, D. melanogaster |
| Detection | ECL |
"The research of our lab aims to understand the mechanisms of maternally deposited RNAs and proteins clearance during maternal-to-zygotic transition (MZT). This is a very good AB, tested on WB only. The image shows GFP-tagged Me31B. Very good and clean. Clean and sharp band. Recommend Boster antibody production service."
Source: Biocompare.com
| SKU | DZ07988 |
|---|---|
| Application | Immunofluorescence |
| Sample | Zebrafish embryos, 5 days old |
| Detection | Confocal Microscopy |
"The antibody clearly labelled the outer segments of photoreceptor cells. The antibody is clean and precise. A zebrafish specific antibody for cdhr1a that WORKS!"
Source: Biocompare.com
| SKU | DZ02095 |
|---|---|
| Application | Immunofluorescence |
| Sample | Zebrafish embryos 5dpf |
| Detection | Confocal Microscopy |
"Antibody labels the retina outer segments and inner nuclear layers as expected based on in situ hybridization studies. The staining is clean. A zebrafish-specific antibody for Siah1 that actually WORKS!"