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| SKU | PB9094 |
|---|---|
| Application | Immunohistochemistry (paraffin-embedded) |
| Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
| Sample | Human Bowel |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Ab Incubation | 37°C for 30 min |
| Primary Ab Incubation diluent | 5% BSA in TBS |
| Primary Ab Concentration | 1ug/ml |
| Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
| Secondary Ab Dilution | The kit was ready to use, no dilution needed |
| Secondary Ab Incubation | at 37°C for 30 min |
Source: Biocompare.com
| SKU | EK0392 |
|---|---|
| Application | ELISA |
| Sample | "Culture media supernatant" of transformed cultured cells of human conjunctiva epithelial cells |
| Detection | -- |
"I am very much satisfied with the product Human IL-1 Beta PicoKine™ ELISA Kit (EK0392) purchased from Boster Bio. I used the Boster Bio ElISA kit for the first time and I am very happy with this product. After trying the IL-1beta kit, I have purchased two other cytokine detection ELISA kits from Boster Bio. The methodology of Human IL-1 Beta PicoKine™ ELISA Kit is simplified and user friendly. It required minimum optimization for my experiments. The written instructions provided with the kit is very useful for running the ELISA. The results obtained so far have been consistent and reproducible. For my future ELISA experiments, I'll be buying the ELISA kit from Boster Bio."
Source: Biocompare.com
| SKU | PB10032 |
|---|---|
| Applications | Western Blot |
| Sample | Human liver, rat liver, mouse liver, mouse kidney, and mouse brain |
| Detection | Typhoon 940 |
"We are studying the metabolism of amino acid and we are interested in knowing if the pathway involved this protein has changed under the background of a knockout mouse. The western blot image for this antibody looks clean and the signal looks strong. The band shown on western blot is at the right size and the intensity is strong enough. Equally important, the price is generally lower than other companies. This antibody works ok in western blot experiments with human, rat, and mouse tissues."
Source: Customer Feedback Submission
| SKU | PA1373 |
|---|---|
| Applications | Western Blot |
| Sample | Mouse brain lysate |
| Detection | Licor Imaging and ImageJ |
"We have tested PA1373 trial sample in our lab and it works far better compared to other vendors. We are extremely happy to get the results. We looked for 5HT2A expression in various brain regions. Our bands show clear expression level of 5HT2A. We now plan to buy this antibody for all our laboratory needs."
Source: Biocompare.com
| SKU | EK0373 |
|---|---|
| Applications | ELISA (Inflammatory cytokine assay from plasma) |
| Sample | Rat plasma |
| Detection | -- |
"I used this assay kit for detection of the cytokines in the plasma samples. Only 100 uL of sample yielded absolutely flawless results. Plasma samples showed variable cytokine expression among independent groups which demonstrates the accuracy of this kit. Straightforward protocol. Very easy to perform. Highly recommended product for precise cytokine assay analysis." (Customer’s Tip: "Keep working area clean. Prepare necessary dilution of the sample before starting the procedure.")
Source: Biocompare.com
| SKU | PA1378-1 |
|---|---|
| Applications | Western Blot |
| Sample | MDA-MB-468 and HEK293E cell lysates |
| Detection | SuperSignal West Pico Plus with a BioRad imager |
"I've been looking at downstream products of AKT and mTOR transcriptional regulation. I tried several anti-MDM2 antibodies from other companies with either ambiguous or negative results (in Western blots of human cancer cell line lysates) and Boster's is the only one that gave me strong bands at the two expected sizes. I would recommend this product. I would absolutely buy it again."
Source: Biocompare.com
| SKU | EK1340 |
|---|---|
| Applications | ELISA |
| Sample | Cell lysates from HUVEC treated with control siRNA or ADAMTS-13 siRNA |
| Detection | -- |
"I used the kit to test the ADAMTS1 protein level in human endothelial cell lysates. The instructions are clear and the kit worked the first time with reproducible results. Decent expression level was detected and results are reproducible. Cheaper than most comparable product. I will keep using this product and will be happy to recommend to my colleagues." (Customer’s Tip: "Just follow the protocol.")
Source: Biocompare.com
| SKU | EK0762 |
|---|---|
| Applications | ELISA |
| Sample | Human ovarian cancer ascites |
| Detection | -- |
"Human ovarian cancer ascites samples were tested for presence of Galectin-1 protein. Depending on the donor, ascites samples were found to have varying concentrations of Gal-1. Straightforward and easy to use once a general idea of Gal-1 concentration in sample of interest is optimized." (Customer’s Tip: "If using for the first time, plate samples at least 3-4 dilutions to get consistent results among samples at the same dilution.")
Source: Biocompare.com
| SKU | PB9212 |
|---|---|
| Applications | Western Blot |
| Sample | Whole cell extract of pancreatic tumor cell MIA PaACa2 |
| Detection | Enhanced chemiluminescence |
"Thirty micrograms of whole cell extract of pancreatic tumor cell MIA PaCa-2 were loaded on a 4-12% Bis-Tris gel. The membrane was blotted with 1xPBS + 0.2% Tween-20 + 10% non-fat dry milk. The membrane was incubated with Anti-HMOX1 Antibody (PB9212) at the concentration of 200 ng/ml at room temperature for 3 hours. The membrane was then blotted with HRP-conjugated anti-rabbit secondary antibodies at 4oC overnight. The band was detected using regular ECL with 1 minute exposure. A major band with 32 kD was detected."
Source: Biocompare.com
| SKU | PA2088 |
|---|---|
| Applications | Immunohistochemistry |
| Sample | Mouse astrocytes |
| Detection | Confocal/Fluorescent Scope |
"The general aim of the experiment was to determine molecular differences of astrocytes in the central nervous system. We wanted to see if there are different expression levels of Sparcl1 in different regions of the mouse brain as a way to compare and possibly validate qPCR data. The product was found on One World Lab and it worked so we decided to buy a greater quantity as opposed to a trial size. The bottom line: Good."