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Source: Biocompare.com
| SKU | PA1239 |
|---|---|
| Applications | Immunohistochemistry |
| Sample | Spinal cord |
| Detection | Confocal microscope |
"I used it for IHC on frozen sections at a dilution of 1:500. It did not need several trials to optimize the protocol. No bad things overall. I will purchase it again."
Source: Biocompare.com
| SKU | EK1232 |
|---|---|
| Applications | Protein level screen |
| Sample | Cell culture media from MCF7 cells |
| Detection | -- |
"The TFF1 ELISA kit from Boster was competitively priced and worked with cell culture media taken from MCF7 cells, a human mammary carcinoma which is well known for overexpression of the TFF1 protein. The kit is very sensitive, detecting TFF1 protein at levels of 10pg/mL. The kit is saturated about 1mg/mL, so in my case, a 1 to 100 dilution of cell culture media was needed in order to be in the linear range of the assay. Good ELISA kit for human TFF1 protein. Worked as advertised."
Source: Biocompare.com
| SKU | PB9323 |
|---|---|
| Applications | Western Blot |
| Sample | MEFs |
| Detection | LICOR Odyssey |
"The goal was to confirm the knockdown of mouse Rad51 via siRNA. Confirmed siRNA knockdown expression. Good detection. Recommended."
Source: Biocompare.com
| SKU | PA1747 |
|---|---|
| Applications | Western Blot |
| Sample | Jurkat Lysates |
| Detection | ECL solution |
"Our lab is identifying IL-2 receptor components in non-lymphoid primary cell lines. We use Jurkat (E6-1) cell lines as a positive control for our experiments. Got one single band at the correct size. Very sensitive, can use at low concentration. Great antibody. Works well with whole cell lystes from our cell lines."
Source: Biocompare.com
| SKU | PA2023 |
|---|---|
| Applications | Western Blot |
| Sample | Jurkat cell lysate |
| Detection | Chemiluminescence |
"We tested several anti-DISC1 antibodies that are commercially available on the market. However, we found that this rabbit polyclonal anti-DISC1 antibody from Bosterbio company is the best one. We have used this antibody for IF, WB and IP purposes in NIH3T3 and Jurkat cell lines...The antibody detected a single band of protein at around 100 KD. Cheap, polyclonal, multipurpose use, fast shipping...The best DISC1 antibody in the market that can be used for IP, IF and WB."
Source: Biocompare.com
| SKU | EK0397 |
|---|---|
| Applications | Detection of secreted IL-2 in media of Jurkat T cells |
| Sample | Jurkat T cell media (RPMI, 10% FBS, 1% pen/strep) |
| Detection | -- |
"I was using this kit to detect IL-2 secretion from Jurkat T-cells upon activation with PMA and PHA. I followed the protocol exactly. I had extremely low levels of IL-2 in my experiment, which were just above background. In this case, it was essential for me to use the standards and blanks to ensure that my samples were above background. All components come with the kit, unlike some other kits from other companies in which only some of the necessary components are provided. This is a great kit that is a bit expensive, but worth the cost for an easy-to-use and sensitive assay."