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Western blot analysis was performed using the GP9 antibody to detect GP9 protein expression in the mouse hippocampus. This antibody is highly efficient and specific, making it suitable for quantitative WB detection of GP9 in mouse tissues.

Excellent, submitted by on
SKU M00024-1
Application Western Blot
Sample Mouse hippocampus tissue
Sample Processing Description The mouse hippocampus was lysed with RIPA buffer containing a protease inhibitor cocktail. After protein quantification, samples were mixed with 5× protein loading buffer and heated for 10 minutes to denature. Load 5 μL of protein per lane and apply to SDS-PAGE.
Primary Antibody Anti-AKT1 Monoclonal Antibody
Primary Incubation overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system:Tanon
Results Summary CD71 antibody was used to detect protein expression in mouse uterine tissue. The bands were clear and free of non-specific signals. After recovery, the antibody could be reused and still showed good performance.

A strong nuclear signal for Cyclin E was observed in proliferating cells by IF. Results were reproducible and matched the expected patterns.

Excellent, submitted by on
SKU DZ41310
Application Immunofluorescence
Sample Human HaCaT cell
Sample Processing Description Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes and blocked in 1% BSA for 1 hour before incubation with the primary antibody.
Primary Antibody Human CCNE1 Antibody
Primary Incubation 1:100-1:200, overnight at 4 ℃
Secondary Antibody Goat anti-rabbit IgG conjugated to Alexa Fluor 488
Secondary Incubation 1:1000, 1-2 hours in room temperature
Other Reagents used PBS, 0.1% Triton X-100, 1% BSA, DAPI for nuclear counterstaining
Detection Fluorescence microscopy using AlexaFluor488 (excitation 488 nm, emission 519 nm) using Zeiss LSM 900 Confocal Microscope with Airyscan 2
Results Summary The CyclinE-N15 antibody (DZ41310) worked fine in IF application using human cell lines. In IF, the nuclear localization of Cyclin E matched the known expression pattern. A fluorescent secondary antibody (Alexa Fluor 488) was used for detection and gave clear signal. Overall, a reliable antibody for D15 transcript Cyclin E detection in human cell systems.

Using freshly prepared blocking buffer helped reduce background. Results were reproducible and matched the expected patterns.

Excellent, submitted by on
SKU DZ41310
Application Western Blot
Sample Human HaCaT cell, Human A2780 cell, Human HCT cell
Sample Processing Description Dissection, Homogenization, Sample boiling in 1X Lamelli, SDS-PAGE, Standard Western Blotting
Primary Antibody Human CCNE1 Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody Anti-rabbit/mouse IgG horseradish peroxidase-conjugated
Secondary Incubation 1:10000, 1-2 hours in room temperature
Detection Biorad Chemidoc
Results Summary Using freshly prepared blocking buffer helped reduce background. Results were reproducible and matched the expected patterns.

Was able to detect tfap2a expression in the retinal ganglion cells and anterior segment at 3dpf.

Excellent, submitted by on
SKU DZ41119
Application Immunofluorescence
Sample Zebrafish retinal cryo-section
Sample Processing Description Embryos fixed in 4% PFA for 4h. Embryos washed in PBST and 30% then 50% sucrose. Embedded in OCT and cryo-sectioned at 20nm
Primary Antibody Zebrafish Tfap2a Antibody
Primary Incubation 1:100, overnight at 4 ℃
Detection Used a Nikon C2+ confocal microscope
Results Summary Was able to detect tfap2a expression in the retinal ganglion cells and anterior segment at 3dpf.

In this study, this antibody was mainly used for Western Blot experiments. In the Western Blot results, the CCT3 antibody showed a clear and specific target band and maintained a strong signal even after multiple rounds of reuse.

Excellent, submitted by on
SKU PB9926
Application Western Blot
Sample U2OS cells
Primary Incubation 1:1000
Blocking Agent BSA
Secondary Antibody Anti-Rabbit IgG Secondary Antibody, HRP-conjugated
Results Summary I will definitely purchase BosterBio products again and recommend them to my classmates and colleagues.

Thank you for all the work on this project

Excellent, submitted by on
SKU DZ01481
Application Immunofluorescence
Sample wildtype and KO mutant zebrafish retina
Primary Incubation 1:200
Blocking Agent 10% normal goat serum and 2% bovine serum albumin in PBS
Secondary Antibody Alexa Fluor 568 goat anti-rabbit was used in blocking solution.

The antibody performs efficiently and specifically, with very few nonspecific bands.

Excellent, submitted by on
WB result image
SKU A03213-2
Application Western Blot
Sample MCF-7 cell
Sample Processing Description Cells were directly lysed in NP-40 buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98 °C. Then, 20 µL of protein sample was loaded per lane onto SDS-PAGE.
Primary Antibody UBA6 Antibody
Primary Incubation 1:1000, overnight at 4 °C
Blocking Agent 5% Non-fat milk
Secondary Antibody HRP-conjugated Goat Anti-Rabbit IgG
Secondary Incubation Incubate at room temperature for 1 hour
Detection Signal was developed using ECL substrate on a Tanon system.
Results Summary The antibody performs efficiently and specifically, with very few nonspecific bands.

Works very well in Western blot for rat enteric glial cells and mouse FGL-2 protein, with only slight nonspecific bands.

Excellent, submitted by on
SKU A06303-3
Application Western Blot
Sample rat enteric glial cells and mouse tissue
Sample Processing Description Samples (5 µL per lane) were run on 10% resolving and stacking gels at 80 V for 20 min, then 150 V for 70 min, and transferred at 400 mA for 100 min.
Primary Incubation The membrane was incubated with the FGL-2 primary antibody (1:1000) overnight at 4 °C.
Secondary Antibody HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody
Secondary Incubation Incubate at room temperature for 2 hour
Other Reagents used 5% non-fat milk
Detection Signal was developed using ECL substrate on an ImageQuant LAS4000mini system.
Results Summary Works very well in Western blot for rat enteric glial cells and mouse FGL-2 protein, with only slight nonspecific bands.

The SP antibody demonstrated strong performance across all tested dilutions, producing clear and specific signal detection.

Excellent, submitted by on
SKU DZ41720
Application Western Blot
Sample HEK293T whole cell lysates
Sample Processing Description HEK293T cells were transfected with a plasmid expressing SP only (and not Env or Rem) and lysed using RIPA buffer. A total of 80 µg of protein was resolved on a 4–12% gradient SDS-PAGE gel and transferred to a membrane. The membrane was blocked for 1 hour at room temperature with 5% non-fat milk in PBS-Tween (PBS-T). After washing three times with 1× PBS-T (10 minutes each), the membrane was incubated overnight at 4 °C with varying dilutions ofthe SP antibody prepared in 2% non-fat milk (1:500; 1:1000; 1:2000). Following three additional washes with PBS-T (10 minutes each), the membrane was incubated with anti-rabbit secondary antibody, washed again three times, and developed using ECL Plus.
Primary Incubation The membrane was incubated with the SP primary antibody (1:500; 1:1000; 1:2000) overnight at 4 °C.
Secondary Antibody Anti-rabbit-HRP-conjugated secondary antibody
Secondary Incubation Dilution: 1:25,000 in PBS-T/2% milk
Other Reagents used 1× RIPA lysis buffer
1× PBS-T washing buffer (T = 1% Tween 20)
Non-fat milk (for blocking and antibody dilution)
Anti-rabbit HRP-conjugated secondary antibody
Detection Signal was developed using ECL Plus chemiluminescent substrate
Results Summary The SP antibody demonstrated strong performance across all tested dilutions, producing clear and specific signal detection. Dilutions of 1:1000 and 1:2000 yielded the most optimal results, showing minimal background compared to 1:500, which highlights the antibody’s high specificity and sensitivity at these concentrations. To ensure equal protein loading across lanes, the membrane was also probed with a β-actin antibody, confirming equal sample loading and validating the observed signal intensity for SP. Overall, these results demonstrate that the SP antibody is reliable for detecting SP in RIPA-lysed HEK293T samples.

The CLIPB4 antibody was efficiently used to detect CLIPB4 protein secreted in the mosquito hemolymph. The protein migrates at ~40 kDa in SDS-PAGE.

Excellent, submitted by on
CLIPB4 antibody Western blot result
SKU DZ33989-1
Application Western Blot
Sample β-galactosidase gene knockdown mosquito hemolymph, CLIPB4 knockdown mosquito hemolymph
Primary Incubation Overnight at 4°C
Secondary Incubation For 1 hour at room temperature
Secondary Antibody Dilution 1:3000
Detection Clarity Max Western ECL substrate
Results Summary Image showing the specificity of CLIPB4 antibody. Lane 1 includes hemolymph extracted from mosquitoes silenced for the bacterial β-galactosidase gene. Lane 2 contains hemolymph extracted from mosquitoes silenced for the CLIPB4 gene.