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Source: Biocompare.com
| SKU | DZ02095 |
|---|---|
| Application | Immunofluorescence |
| Sample | Zebrafish embryos 5dpf |
| Detection | Confocal Microscopy |
"Antibody labels the retina outer segments and inner nuclear layers as expected based on in situ hybridization studies. The staining is clean. A zebrafish-specific antibody for Siah1 that actually WORKS!"
Source: Biocompare.com
| SKU | DZ12892 |
|---|---|
| Application | Immunofluorescence |
| Sample | Zebrafish embryos 24hpf |
| Detection | Confocal Microscopy |
"Zebrafish-specific antibody. Used in whole mount IF on 24hpf zebrafish embryos. The signal is not precisely clean, but we do observe nlz2 expression in the ventral region of the retina as expected from in situ hybridization results. This antibody shows some promising results in whole mount applications using 24hpf embryos. Applications with cryosections may be more precise."
Source: Biocompare.com
| SKU | EK0527 |
|---|---|
| Application | ELISA |
| Sample | Mouse serum |
| Detection | -- |
"The kit contained an extra standard which is very useful if you need to optimize your dilution factor before you use the rest of your samples. The instructions were very easy to follow and the results we obtained were consistent between replicates. Very easy to use. Don't waste your time or money [on other kits]; just use this kit." (Customer’s Tip: "Optimization dilution factors should range from 1:5 to 1:200.")
Source: Biocompare.com
| SKU | EK0411 |
|---|---|
| Application | ELISA |
| Sample | Mouse serum |
| Detection | -- |
"The kit contained an extra standard which is very useful if you need to optimize your dilution factor before you use the rest of your samples. The instructions were very easy to follow and the results we obtained were consistent between replicates. Very easy to use. Don't waste your time or money [on other kits]; just use this kit." (Customer’s Tip: "Optimize dilutions 1:5-1:250.")
Source: Biocompare.com
| SKU | EK0309 |
|---|---|
| Applications | ELISA |
| Sample | Brain Tissue Lysate |
| Detection | -- |
"I used the ELISA kit to detect the BDNF level in several samples, including brain tissues (cortex and hippocampus), cultured astrocyte (cell lysis) and astrocyte conditioned medium. The kit works very well for cell lysis and brain tissues according to the instruction. For the cell medium, I suggest that users need modify the protocol a little bit, such as prolong the incubation time for samples in the wells for overnight. The kit has a good quality."
Source: Biocompare.com
| SKU | EK0307 |
|---|---|
| Application | ELISA |
| Sample | Cell lysate and culture medium |
| Detection | -- |
"The kit was used to quantify amount of BDNF produced after transfection with plasmid DNA encoding human BDNF using non viral vector, liposomes. Provided good reliable quantification of BDNF produced. No interference. Good reproducibility. Reliable estimation, good sensitivity. Reasonable price as well. Would purchase again." (Customer’s Tip: "Don't dilute sample, assay directly.")
Source: Biocompare.com
| SKU | EK0596 |
|---|---|
| Application | ELISA |
| Sample | Serum, tissue homogenate |
| Detection | -- |
"I used this kit to quantify adiponectin adipokine in cell culture supernatant (3T3-L1 cells) and mouse serum (C57BL6 mice on control/ western diet). The goal was to check if western diet treatment reduced serum and tissue adiponectin levels. The results were reasonable and reproducible. Although we had to use two different standard curves for high and low concentration estimations. Performing a standard curve with each sample helped. Reliable estimation, good supplier, reasonable price. Will keep using it." (Customer’s Tip: "Dilute tissue homogenate 1:100, use serum directly.")
Source: Biocompare.com
| SKU | EK0411 |
|---|---|
| Application | ELISA |
| Sample | Cell culture media |
| Detection | -- |
"Good estimation of IL-6. The results were comparable and reproducible each time. Good brand name, very good quality of reagents. Overall justified results with quality of the product. Also good value for the money. Order 4 or more kits to get good discount. That was helpful in my case. Will definitely purchase again throughout my project." (Customer’s Tip: "If performing for 6-well plate supernatant, may run a little higher in concentration. Consider diluting samples on doing a test run.")
Source: Biocompare.com
| SKU | EK0527 |
|---|---|
| Application | ELISA |
| Sample | Cell culture media, mouse serum |
| Detection | -- |
"I was able to estimate TNFa in cell culture media before and after treatment with lipopolysaccharides in primary macrophage cell line. In vivo estimation was done in control and treated group of mice (high fat diet treatment) by collecting blood, separating serum, and estimating the cytokine levels in serum. Overall reliable estimation was achieved. Reliable reproducible result and brand of ELISA kits. Good ELISA kit. Reliable vendor. Good continuity between different batches of ELISA kits. Will continue to buy from Boster Biological." (Customer’s Tip: "Do a test run of sample and quantities may be higher than the standard plot range.")
Source: Biocompare.com
| SKU | EK0568 |
|---|---|
| Application | ELISA |
| Sample | Centrifuged cell culture media supernatant |
| Detection | -- |
"Had to perform the analysis twice initially as the background wasn't clear. Overall, good reproducible estimation. Reliable, reproducible. Good product but would appreciate a faster assay kit with less chances of manual error." (Customer’s Tip: "Perform washing steps at least 6-7 times to get a clearer background with 60 seconds wait period in between each washing.")