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WB results showed that VEGFC (M00623) was upregulated by LPS and reduced after dexamethasone or homemade drug treatment, with clear and specific bands, indicating an anti-inflammatory effect.

Excellent, submitted by Kaitong Yang, Jiangnan University, Wuxi Medical College on 2026-03-04

SKU	M00623
Application	Western Blot
Sample	Human retinal microvascular endothelial cells
Sample Processing Description	Human retinal microvascular endothelial cells treated with LPS and then with dexamethasone or homemade drug.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody	VEGFC Antibody
Primary Incubation	1:2000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation	1:10000, 1 h in RT
Detection	Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary	VEGFC is an important signaling protein in vivo that primarily acts on lymphatic and blood vascular endothelial cells, promoting lymphangiogenesis and angiogenesis. WB results showed that VEGFC expression was increased in LPS-stimulated human retinal microvascular endothelial cells and decreased after treatment with dexamethasone or a homemade drug, indicating the anti-inflammatory effect of the homemade drug.

WB results showed that TPH1 (A01626-1) was upregulated in the rat colon model group and reduced after Chinese medicine treatment, with the high-dose group showing the best effect, and the target bands were clear and specific.

Excellent, submitted by Shiyu Zhang, Liaoning University of Traditional Chinese Medicine on 2026-03-04

SKU	A01626-1
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	Rat colon tissues were lysed in RIPA buffer containing PMSF (100:1) for 10 min, centrifuged at 12,000 rpm for 15 min, and the supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 min, and then loaded onto SDS-PAGE.
Other Reagents	5% non-fat milk
Primary Antibody	Tryptophan Hydroxylase/TPH1 Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation	1:5000, 1 h in RT
Detection	Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary	The figure shows WB results of TPH1 and the internal control Actin in rat colon across different groups; expression was elevated in the model group and reduced after Chinese medicine treatment, with the high-dose group showing the best effect, and the target bands were clear and specific, indicating satisfactory results.

WB results showed that CCNB1 (A00745-1) was upregulated in the rat colon model group and reduced after treatment, with clear and specific bands.

Excellent, submitted by Shiyu Zhang, Liaoning University of Traditional Chinese Medicine on 2026-03-04

SKU	A00745-1
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer containing PMSF (100:1) was used for lysis for 10 min, followed by centrifugation at 12,000 rpm for 15 min. The supernatant was collected, mixed with 5× loading buffer, denatured at 100°C for 10 min, and then subjected to SDS-PAGE.
Other Reagents	5% non-fat milk
Primary Antibody	Cyclin B1/CCNB1 Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation	1:5000, 1 h in RT
Detection	Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary	The figure shows WB results of CCNB1 and the internal control Actin in rat colon across different groups; CCNB1 expression was elevated in the model group and decreased after traditional Chinese medicine treatment, with the high-dose group showing the best effect, and the target bands were clear and specific, indicating satisfactory results.

Anti-PRLR Antibody (PA2087) demonstrated clear and specific detection of PRLR in mouse brain tissue by Western blot, with distinct differences observed among the control, model, and AB treatment groups.

Excellent, submitted by Yetao Ju, Liaoning University of Traditional Chinese Medicine on 2026-02-28

SKU	PA2087
Application	Western Blot
Sample	mouse brain tissue
Sample Processing Description	Mouse brain tissues were lysed in RIPA buffer containing a protease inhibitor cocktail at 4 °C for 2 hours. After centrifugation, the supernatant was collected for protein quantification. The protein concentration was adjusted accordingly, mixed with 5× protein loading buffer, and denatured by heating for 10 minutes. Then, 15 μl of protein sample was loaded per lane for electrophoresis.
Other Reagents	blocking buffer
Primary Antibody	Prolactin Receptor/PRLR Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation	1:2000, 1 h in RT
Detection	Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary	The figure shows representative Western blot results of PRLR and the internal control β-actin in brain tissues from normal mice, the model group, and mice treated with low and high doses of AB. The antibody produced clear bands, and distinct differences among the experimental groups were clearly observed.

Western blot of OCI-LY1 cells treated with SPHINX31 showed stable GAPDH expression with clear bands and clean background, demonstrating high specificity of A00227-1.

Excellent, submitted by Maolin Yao, Fujian Medical University on 2026-02-27

SKU	A00227-1
Application	Western Blot
Sample	human OCI-LY1 cellls
Sample Processing Description	Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio, boiled for 10 minutes, and 15 μL of protein was loaded per well.
Other Reagents	5% non-fat milk
Primary Antibody	GAPDH Antibody Picoband®
Primary Incubation	1:5000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1 h in RT
Detection	Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary	In OCI-LY1 cells treated with different concentrations of SPHINX31 for 24 h, GAPDH expression remained stable with no significant differences, showing clear bands and a clean background without nonspecific signals.

The Anti-GAPDH antibody (A00227-1) demonstrated high sensitivity and clear WB bands in mouse intestinal tissue, offering excellent cost-effectiveness and reliability, and is highly recommended for use.

Excellent, submitted by Qihang Hou, China Agricultural University on 2026-02-27

SKU	A00227-1
Application	Western Blot
Sample	mouse intesinal tissue
Sample Processing Description	Mouse colon tissue was lysed in RIPA buffer containing a protease inhibitor cocktail. Protein concentration was determined using the Pierce™ BCA Protein Assay Kit, and equal amounts of protein were loaded after boiling denaturation.
Other Reagents	5% non-fat milk
Primary Antibody	GAPDH Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	goat anti rabbit secondary antibodies
Secondary Incubation	1:5000, 1 h in RT
Detection	Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary	This antibody is highly sensitive, produces clear WB bands, is reusable, offers excellent cost-effectiveness, and demonstrates a clear advantage over similar international products, making it highly recommended for use.

In WB using Anti-CDK1 (Phospho-T450) antibody (Cat# PB9533-50 µL), CDK1 expression in rat colon was increased in the model group and most effectively reduced in the high-dose herbal treatment group, with clear and well-defined target bands.

Excellent, submitted by Shiyu Zhang, Liaoning University of Traditional Chinese Medicine on 2026-02-26

SKU	PB9533
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	Samples were lysed in RIPA buffer containing PMSF protease inhibitor (100:1) for 10 min, centrifuged at 12,000 rpm for 15 min, and the supernatant was mixed with 5× loading buffer, boiled at 100 °C for 10 min, and loaded onto SDS-PAGE.
Other Reagents	5% Non-fat milk
Primary Antibody	CDK1 Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L)
Secondary Incubation	1:5000, 1 h in RT
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The image shows WB results of CDK1 and the loading control Actin in rat colon across different groups; CDK1 expression was elevated in the model group and most effectively reduced in the high-dose herbal treatment group, with clear and distinct target bands.

In WB using Occludin antibody (Cat# A01246), clear bands showed decreased Occludin in the model group and best recovery in the high-dose herbal treatment group.

Excellent, submitted by Shiyu Zhang, Liaoning University of Traditional Chinese Medicine on 2026-02-26

SKU	A01246
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer containing protease inhibitor PMSF (100:1) was used to lyse samples for 10 min, followed by centrifugation at 12,000 rpm for 15 min; the supernatant was mixed with 5× loading buffer, boiled at 100 °C for 10 min, and loaded onto SDS-PAGE.
Other Reagents	5% Non-fat milk
Primary Antibody	Occludin OCLN Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L)
Secondary Incubation	1:5000, 1 h in RT
Detection	Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary	The image shows WB results of Occludin and the loading control Actin in rat colon across different groups; Occludin expression was reduced in the model group, with the high-dose herbal treatment showing the best recovery and clear, well-defined target bands.

In this Western blot experiment using Anti-AQP1 antibody (Cat# BM5035) on MADB106 rat mammary carcinoma cells, AQP1 protein levels were markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor.

Excellent, submitted by Xinshuo Wang, Xi’an Jiaotong University on 2026-02-25

SKU	M00865
Application	Western blot
Sample	MADB106 rat mammary carcinoma cells
Sample Processing Description	MADB106 cells under normal culture conditions , MADB106 cells treated with agonist , MADB106 cells treated with inhibitor
Other Reagents	RIPA Lysis Buffer, Protease Inhibitor, Resolving Gel Solution ,Transfer Buffer ,Blocking Buffer
Primary Antibody	AQP1 Rabbit Monoclonal Antibody
Primary Incubation	1:3000, overnight at 4 ℃
Secondary Antibody	1:10,000, HRP-conjugated Goat Anti-Rabbit IgG
Secondary Incubation	1h at RT
Detection	Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary	AQP1 (Aquaporin 1) is the first discovered water channel protein, whose primary function is efficient water transport. Recent studies have revealed that AQP1 plays a critical role in cancer progression, promoting tumor growth in breast cancer by enhancing angiogenesis and cell migration. In this experiment, AQP1 protein levels were markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor.

In this WB experiment using Anti-CAMP antibody (Cat# A05475-1) on MADB106 rat mammary carcinoma cells, CAMP protein secretion was markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor.

Excellent, submitted by Xinshuo Wang, Xi’an Jiaotong University on 2026-02-25

SKU	A05475-1
Application	Western blot
Sample	MADB106 rat mammary carcinoma cells
Sample Processing Description	MADB106 cells under normal culture conditions , MADB106 cells treated with agonist , MADB106 cells treated with inhibitor
Other Reagents	RIPA Lysis Buffer, Protease Inhibitor, Resolving Gel Solution ,Transfer Buffer ,Blocking Buffer
Primary Antibody	Camp Antibody Picoband®
Primary Incubation	1:2000, overnight at 4 ℃
Secondary Antibody	1:10,000, HRP-conjugated Goat Anti-Rabbit IgG
Secondary Incubation	1h at 37℃
Detection	Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary	CAMP, also known as cathelicidin antimicrobial peptide or LL-37, is an important antimicrobial peptide with dual functions: directly killing pathogens and modulating immune responses. It plays a complex role in maintaining the homeostasis of barrier tissues such as skin and in influencing tumor progression. In breast cancer, CAMP may promote tumor development by enhancing angiogenesis and cancer cell proliferation. In this experiment, CAMP secretion was markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor.