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Western blot using Anti-ER/ESR1 Antibody (M00057-2) in mouse brain tissues showed clear, specific ESR1 bands across normal, disease model, and AB drug-treated groups, with β‑actin as a consistent loading control.

Excellent, submitted by on
SKU M00057-2
Application Western Blot
Sample mouse brain tissue
Sample Processing Description Mouse brain tissues were lysed in RIPA buffer containing a protease inhibitor cocktail at 4°C for 2 hours, centrifuged to collect the supernatant, and protein concentration was determined. After adjusting the concentration, samples were mixed with 5× protein loading buffer, denatured by heating at 95–100°C for 10 minutes, and 15 μL of protein was loaded per lane for SDS-PAGE.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody goat anti rabbit secondary antibodies
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary This antibody is highly sensitive, produces clear WB bands, is reusable, offers excellent cost-effectiveness, and demonstrates a clear advantage over similar international products, making it highly recommended for use.

WB results showed that VEGFC (M00623) was upregulated by LPS and reduced after dexamethasone or homemade drug treatment, with clear and specific bands, indicating an anti-inflammatory effect.

Excellent, submitted by on
SKU M00623
Application Western Blot
Sample Human retinal microvascular endothelial cells
Sample Processing Description Human retinal microvascular endothelial cells treated with LPS and then with dexamethasone or homemade drug.
Other ReagentsRIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody VEGFC Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:10000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary VEGFC is an important signaling protein in vivo that primarily acts on lymphatic and blood vascular endothelial cells, promoting lymphangiogenesis and angiogenesis. WB results showed that VEGFC expression was increased in LPS-stimulated human retinal microvascular endothelial cells and decreased after treatment with dexamethasone or a homemade drug, indicating the anti-inflammatory effect of the homemade drug.

WB results showed that TPH1 (A01626-1) was upregulated in the rat colon model group and reduced after Chinese medicine treatment, with the high-dose group showing the best effect, and the target bands were clear and specific.

Excellent, submitted by on
SKU A01626-1
Application Western Blot
Sample rat colon tissue
Sample Processing Description Rat colon tissues were lysed in RIPA buffer containing PMSF (100:1) for 10 min, centrifuged at 12,000 rpm for 15 min, and the supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 min, and then loaded onto SDS-PAGE.
Other Reagents5% non-fat milk
Primary Antibody Tryptophan Hydroxylase/TPH1 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary The figure shows WB results of TPH1 and the internal control Actin in rat colon across different groups; expression was elevated in the model group and reduced after Chinese medicine treatment, with the high-dose group showing the best effect, and the target bands were clear and specific, indicating satisfactory results.

WB results showed that CCNB1 (A00745-1) was upregulated in the rat colon model group and reduced after treatment, with clear and specific bands.

Excellent, submitted by on
SKU A00745-1
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer containing PMSF (100:1) was used for lysis for 10 min, followed by centrifugation at 12,000 rpm for 15 min. The supernatant was collected, mixed with 5× loading buffer, denatured at 100°C for 10 min, and then subjected to SDS-PAGE.
Other Reagents 5% non-fat milk
Primary Antibody Cyclin B1/CCNB1 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary The figure shows WB results of CCNB1 and the internal control Actin in rat colon across different groups; CCNB1 expression was elevated in the model group and decreased after traditional Chinese medicine treatment, with the high-dose group showing the best effect, and the target bands were clear and specific, indicating satisfactory results.

Anti-PRLR Antibody (PA2087) demonstrated clear and specific detection of PRLR in mouse brain tissue by Western blot, with distinct differences observed among the control, model, and AB treatment groups.

Excellent, submitted by on
SKU PA2087
Application Western Blot
Sample mouse brain tissue
Sample Processing Description Mouse brain tissues were lysed in RIPA buffer containing a protease inhibitor cocktail at 4 °C for 2 hours. After centrifugation, the supernatant was collected for protein quantification. The protein concentration was adjusted accordingly, mixed with 5× protein loading buffer, and denatured by heating for 10 minutes. Then, 15 μl of protein sample was loaded per lane for electrophoresis.
Other Reagents blocking buffer
Primary Antibody Prolactin Receptor/PRLR Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:2000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary The figure shows representative Western blot results of PRLR and the internal control β-actin in brain tissues from normal mice, the model group, and mice treated with low and high doses of AB. The antibody produced clear bands, and distinct differences among the experimental groups were clearly observed.

Western blot of OCI-LY1 cells treated with SPHINX31 showed stable GAPDH expression with clear bands and clean background, demonstrating high specificity of A00227-1.

Excellent, submitted by on
SKU A00227-1
Application Western Blot
Sample human OCI-LY1 cellls
Sample Processing Description Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio, boiled for 10 minutes, and 15 μL of protein was loaded per well.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:5000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary In OCI-LY1 cells treated with different concentrations of SPHINX31 for 24 h, GAPDH expression remained stable with no significant differences, showing clear bands and a clean background without nonspecific signals.

The Anti-GAPDH antibody (A00227-1) demonstrated high sensitivity and clear WB bands in mouse intestinal tissue, offering excellent cost-effectiveness and reliability, and is highly recommended for use.

Excellent, submitted by on
SKU A00227-1
Application Western Blot
Sample mouse intesinal tissue
Sample Processing Description Mouse colon tissue was lysed in RIPA buffer containing a protease inhibitor cocktail. Protein concentration was determined using the Pierce™ BCA Protein Assay Kit, and equal amounts of protein were loaded after boiling denaturation.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody goat anti rabbit secondary antibodies
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary This antibody is highly sensitive, produces clear WB bands, is reusable, offers excellent cost-effectiveness, and demonstrates a clear advantage over similar international products, making it highly recommended for use.

Pancreatic IL-6 levels measured using the Boster Anti-IL-6 antibody (PB9034) demonstrated high specificity and sensitivity in WB analysis, providing reliable evidence for evaluating the anti-inflammatory effect of the nanodrug.

Excellent, submitted by on
SKU PB9034
Application Western Blot
Sample mouse PACs cells
Sample Processing Description After centrifugation, the supernatant was collected. A small aliquot of the protein solution was taken for BCA quantification, and the remaining protein solution was mixed with an equal volume of loading buffer, then denatured in a 100 °C water bath for 5 minutes.
Other Reagents Protein-Free Rapid Blocking Buffer
Primary Antibody Anti-IL6 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L)
Secondary Incubation 1 h in RT
Detection Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary The levels of IL-6 and IL-1β in the pancreas are key indicators for evaluating whether the nanodrug exerts anti-inflammatory effects. Initially, antibodies from two domestic and international suppliers were used for WB experiments, but their specificity was relatively poor. Subsequently, the antibody from Boster was adopted, which demonstrated strong specificity, high titer, and cost-effectiveness.

In WB using Anti-CDK1 (Phospho-T450) antibody (Cat# PB9533-50 µL), CDK1 expression in rat colon was increased in the model group and most effectively reduced in the high-dose herbal treatment group, with clear and well-defined target bands.

Excellent, submitted by on
SKU PB9533
Application Western Blot
Sample rat colon tissue
Sample Processing Description Samples were lysed in RIPA buffer containing PMSF protease inhibitor (100:1) for 10 min, centrifuged at 12,000 rpm for 15 min, and the supernatant was mixed with 5× loading buffer, boiled at 100 °C for 10 min, and loaded onto SDS-PAGE.
Other Reagents 5% Non-fat milk
Primary Antibody CDK1 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The image shows WB results of CDK1 and the loading control Actin in rat colon across different groups; CDK1 expression was elevated in the model group and most effectively reduced in the high-dose herbal treatment group, with clear and distinct target bands.

In WB using Occludin antibody (Cat# A01246), clear bands showed decreased Occludin in the model group and best recovery in the high-dose herbal treatment group.

Excellent, submitted by on
SKU A01246
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer containing protease inhibitor PMSF (100:1) was used to lyse samples for 10 min, followed by centrifugation at 12,000 rpm for 15 min; the supernatant was mixed with 5× loading buffer, boiled at 100 °C for 10 min, and loaded onto SDS-PAGE.
Other Reagents 5% Non-fat milk
Primary Antibody Occludin OCLN Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary The image shows WB results of Occludin and the loading control Actin in rat colon across different groups; Occludin expression was reduced in the model group, with the high-dose herbal treatment showing the best recovery and clear, well-defined target bands.