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The Anti-α-SMA antibody (Cat# BM3902) shows clear and specific bands with low background in WB analysis of mouse heart tissues across different ages, with results consistent with expected trends.

Excellent, submitted by on
SKU M01072-1
Application Western Blot
Sample mouse cardiac tissue
Sample Processing Description Mouse cardiac tissues were collected from 3-, 6-, 12-, and 18-month-old mice, and total protein was extracted.
Other Reagents RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:10000, 1 h at RT
Detection Substrate: ECL substrate
Imaging system: ChemiDoc MP
Results Summary This experiment aimed to investigate the changes in α-SMA expression in mouse cardiac tissues from 3-, 6-, 12-, and 18-month-old mice (representing young, middle-aged, and aged stages). The results show that α-SMA levels are generally similar across different ages, with a slight but not significant increase as age progresses.

Western blot analysis using Anti-COL3A1 Antibody (M00788-1) showed clear and specific bands in mouse myocardium samples from 3-, 6-, 12-, and 18-month-old mice, with overall comparable COL3A1 expression and a slight, non-significant increase with age.

Excellent, submitted by on
SKU M00788-1
Application Western Blot
Sample mouse brain tissue
Sample Processing Description Myocardial tissues were collected from mice at 3, 6, 12, and 18 months of age, and total protein was extracted.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, blocking buffer
Primary Antibody Collagen III Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:10000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary This experiment aimed to study the changes in COL3A1 protein in mouse myocardium at 3, 6, 12, and 18 months of age (representing young, adult, middle-aged, and old mice). The results show that COL3A1 levels are largely similar across ages, with a slight, non-significant increase as age progresses.

Western blot using Anti-ER/ESR1 Antibody (M00057-2) in mouse brain tissues showed clear, specific ESR1 bands across normal, disease model, and AB drug-treated groups, with β‑actin as a consistent loading control.

Excellent, submitted by on
SKU M00057-2
Application Western Blot
Sample mouse brain tissue
Sample Processing Description Mouse brain tissues were lysed in RIPA buffer containing a protease inhibitor cocktail at 4°C for 2 hours, centrifuged to collect the supernatant, and protein concentration was determined. After adjusting the concentration, samples were mixed with 5× protein loading buffer, denatured by heating at 95–100°C for 10 minutes, and 15 μL of protein was loaded per lane for SDS-PAGE.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody goat anti rabbit secondary antibodies
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary This antibody is highly sensitive, produces clear WB bands, is reusable, offers excellent cost-effectiveness, and demonstrates a clear advantage over similar international products, making it highly recommended for use.

WB results showed that VEGFC (M00623) was upregulated by LPS and reduced after dexamethasone or homemade drug treatment, with clear and specific bands, indicating an anti-inflammatory effect.

Excellent, submitted by on
SKU M00623
Application Western Blot
Sample Human retinal microvascular endothelial cells
Sample Processing Description Human retinal microvascular endothelial cells treated with LPS and then with dexamethasone or homemade drug.
Other ReagentsRIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody VEGFC Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:10000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary VEGFC is an important signaling protein in vivo that primarily acts on lymphatic and blood vascular endothelial cells, promoting lymphangiogenesis and angiogenesis. WB results showed that VEGFC expression was increased in LPS-stimulated human retinal microvascular endothelial cells and decreased after treatment with dexamethasone or a homemade drug, indicating the anti-inflammatory effect of the homemade drug.

WB results showed that TPH1 (A01626-1) was upregulated in the rat colon model group and reduced after Chinese medicine treatment, with the high-dose group showing the best effect, and the target bands were clear and specific.

Excellent, submitted by on
SKU A01626-1
Application Western Blot
Sample rat colon tissue
Sample Processing Description Rat colon tissues were lysed in RIPA buffer containing PMSF (100:1) for 10 min, centrifuged at 12,000 rpm for 15 min, and the supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 min, and then loaded onto SDS-PAGE.
Other Reagents5% non-fat milk
Primary Antibody Tryptophan Hydroxylase/TPH1 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary The figure shows WB results of TPH1 and the internal control Actin in rat colon across different groups; expression was elevated in the model group and reduced after Chinese medicine treatment, with the high-dose group showing the best effect, and the target bands were clear and specific, indicating satisfactory results.

WB results showed that Cingulin/CGN (A02373-1) was downregulated in the rat colon model group and restored after Chinese medicine treatment, with the high-dose group showing the best effect, and the target bands were clear and specific.

Excellent, submitted by on
SKU A02373-1
Application Western Blot
Sample rat colon tissue
Sample Processing Description Rat colon tissues were lysed in RIPA buffer containing PMSF (100:1) for 10 min, centrifuged at 12,000 rpm for 15 min, and the supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 min, and then loaded onto SDS-PAGE.
Other Reagents5% non-fat milk
Primary Antibody Cingulin/CGN Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary The figure shows WB results of Cingulin/CGN and the internal control Actin in rat colon across different groups; expression was decreased in the model group and restored after Chinese medicine treatment, with the high-dose group showing the best effect, and the target bands were clear and specific, indicating satisfactory results.

WB results showed that CCNB1 (A00745-1) was upregulated in the rat colon model group and reduced after treatment, with clear and specific bands.

Excellent, submitted by on
SKU A00745-1
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer containing PMSF (100:1) was used for lysis for 10 min, followed by centrifugation at 12,000 rpm for 15 min. The supernatant was collected, mixed with 5× loading buffer, denatured at 100°C for 10 min, and then subjected to SDS-PAGE.
Other Reagents 5% non-fat milk
Primary Antibody Cyclin B1/CCNB1 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary The figure shows WB results of CCNB1 and the internal control Actin in rat colon across different groups; CCNB1 expression was elevated in the model group and decreased after traditional Chinese medicine treatment, with the high-dose group showing the best effect, and the target bands were clear and specific, indicating satisfactory results.

Anti-PRLR Antibody (PA2087) demonstrated clear and specific detection of PRLR in mouse brain tissue by Western blot, with distinct differences observed among the control, model, and AB treatment groups.

Excellent, submitted by on
SKU PA2087
Application Western Blot
Sample mouse brain tissue
Sample Processing Description Mouse brain tissues were lysed in RIPA buffer containing a protease inhibitor cocktail at 4 °C for 2 hours. After centrifugation, the supernatant was collected for protein quantification. The protein concentration was adjusted accordingly, mixed with 5× protein loading buffer, and denatured by heating for 10 minutes. Then, 15 μl of protein sample was loaded per lane for electrophoresis.
Other Reagents blocking buffer
Primary Antibody Prolactin Receptor/PRLR Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation 1:2000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary The figure shows representative Western blot results of PRLR and the internal control β-actin in brain tissues from normal mice, the model group, and mice treated with low and high doses of AB. The antibody produced clear bands, and distinct differences among the experimental groups were clearly observed.

Immunofluorescence analysis using the Anti-CD68 antibody (A00602-1) on human normal and preterm placenta showed minimal CD68 expression in normal samples and abundant expression in preterm inflamed samples, consistent with expected results.

Excellent, submitted by on
SKU A00602-1
Application Immunofluorescence
Sample human normal and preterm placenta
Sample Processing Description Human normal-term and preterm placentas were collected clinically and processed for longitudinal paraffin embedding, allowing visualization of both the amnion and chorion membranes.
Other Reagents Goat serum, DAPI, anti-fade mounting medium
Primary Antibody Anti-CD68 Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody DyLight 594-conjugated Donkey Anti-Mouse IgG (H+L)（BA1148）
Secondary Incubation 1:500, 45 min in 37℃
Detection Image system:Leica DM2500
Results Summary In normal placentas, macrophages are scarce or nearly absent, showing only sporadic CD68 expression, whereas in preterm placentas with inflammation, macrophages are abundant and CD68 expression is markedly increased, consistent with expected theoretical data.

Western blot of OCI-LY1 cells treated with SPHINX31 showed stable GAPDH expression with clear bands and clean background, demonstrating high specificity of A00227-1.

Excellent, submitted by on
SKU A00227-1
Application Western Blot
Sample human OCI-LY1 cellls
Sample Processing Description Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio, boiled for 10 minutes, and 15 μL of protein was loaded per well.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:5000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary In OCI-LY1 cells treated with different concentrations of SPHINX31 for 24 h, GAPDH expression remained stable with no significant differences, showing clear bands and a clean background without nonspecific signals.