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In WB using Occludin antibody (Cat# A01246), clear bands showed decreased Occludin in the model group and best recovery in the high-dose herbal treatment group.

Excellent, submitted by on
SKU A01246
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer containing protease inhibitor PMSF (100:1) was used to lyse samples for 10 min, followed by centrifugation at 12,000 rpm for 15 min; the supernatant was mixed with 5× loading buffer, boiled at 100 °C for 10 min, and loaded onto SDS-PAGE.
Other Reagents 5% Non-fat milk
Primary Antibody Occludin OCLN Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary The image shows WB results of Occludin and the loading control Actin in rat colon across different groups; Occludin expression was reduced in the model group, with the high-dose herbal treatment showing the best recovery and clear, well-defined target bands.

In IHC using Ki67 antibody (Cat# PB9026), strong and specific nuclear staining was observed in nude mouse tumor tissue, confirming high Ki67 expression and active cell proliferation.

Excellent, submitted by on
SKU PB9026
Application Western Blot
Sample Nude mouse tumor tissue
Sample Processing Description Paraffin-embedded tumor tissue sections.
Primary Antibody Ki67/MKI67 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL reagent, Imaging system:ChemiDoc MP
Results Summary This antibody is highly specific and efficient, with a clean background and no nonspecific bands. The target band has sharp and well-defined edges.

In this IHC experiment using Anti-SLC22A3 antibody (Cat# M04914), strong and clear expression was observed in cervical cancer tumor cells, with further comparison planned between tumor and adjacent tissues.

Excellent, submitted by on
SKU M04067-2
Application Immunohistochemistry
Sample human cervical cancer tissue
Sample Processing Description Cervical cancer tissue collected from clinical surgery, fixed in formalin and paraffin-embedded
Other ReagentsGoat serum, DAB substrate solution
Primary Antibody SLC22A3 Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Two-step IHC detection kit
Secondary Incubation 30 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary IHC using SLC22A3 antibody (M04914) showed strong and clear expression in tumor cells of human cervical carcinoma, and the next step will be to compare expression between tumor and adjacent normal tissue.

In this IHC experiment using Anti-SERPINB3 antibody (Cat# M04067-2) on human cervical cancer paraffin sections, SERPINB3 was highly expressed in tumor cells with clear positive staining and low background, demonstrating excellent antibody performance.

Excellent, submitted by on
SKU M04067-2
Application Immunohistochemistry
Sample human cervical cancer tissue
Sample Processing Description Cervical cancer tissue collected from clinical surgery, fixed in formalin and paraffin-embedded
Other ReagentsGoat serum, DAB substrate solution
Primary Antibody SerpinB3 Rabbit Monoclonal Antibody
Primary Incubation 1:400, overnight at 4 ℃
Secondary Antibody Two-step IHC detection kit
Secondary Incubation 30 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary The results demonstrated high expression of SERPINB3 in cervical cancer tumor cells with clear positive staining; further studies will compare its expression levels between tumor and adjacent non-tumor tissues.

In this IHC experiment using Anti-CD4 antibody (Cat# M00344) on human cervical cancer paraffin sections, CD4-positive helper T cells were clearly and specifically stained with minimal background, demonstrating excellent antibody performance.

Excellent, submitted by on
SKU M00344
Application Western blot
Sample human cervical cancer paraffin sections
Sample Processing Description Cervical cancer tissue collected from clinical surgery, fixed in formalin and paraffin-embedded
Other ReagentsGoat serum, DAB substrate solution
Primary Antibody CD4 Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Two-step IHC detection kit
Secondary Incubation 30 min at 37℃
Detection Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary CD4 is a marker of helper T cells; in this IHC experiment, CD4 staining clearly identified helper T cells in human cervical cancer samples, showing excellent specificity and performance of the antibody.

In this Western blot experiment using Anti-AQP1 antibody (Cat# BM5035) on MADB106 rat mammary carcinoma cells, AQP1 protein levels were markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor.

Excellent, submitted by on
SKU M00865
Application Western blot
Sample MADB106 rat mammary carcinoma cells
Sample Processing Description MADB106 cells under normal culture conditions , MADB106 cells treated with agonist , MADB106 cells treated with inhibitor
Other ReagentsRIPA Lysis Buffer, Protease Inhibitor, Resolving Gel Solution ,Transfer Buffer ,Blocking Buffer
Primary Antibody AQP1 Rabbit Monoclonal Antibody
Primary Incubation 1:3000, overnight at 4 ℃
Secondary Antibody 1:10,000, HRP-conjugated Goat Anti-Rabbit IgG
Secondary Incubation 1h at RT
Detection Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary AQP1 (Aquaporin 1) is the first discovered water channel protein, whose primary function is efficient water transport. Recent studies have revealed that AQP1 plays a critical role in cancer progression, promoting tumor growth in breast cancer by enhancing angiogenesis and cell migration. In this experiment, AQP1 protein levels were markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor.

In this Western blot (WB) experiment using Anti-AQP5 antibody (Cat# A03085) on MADB106 rat mammary carcinoma cells, AQP5 protein levels were significantly increased with agonist treatment and markedly reduced with inhibitor treatment.

Excellent, submitted by on
SKU A03085
Application Western blot
Sample MADB106 rat mammary carcinoma cells
Sample Processing Description MADB106 cells under normal culture conditions , MADB106 cells treated with agonist , MADB106 cells treated with inhibitor
Other ReagentsRIPA Lysis Buffer, Protease Inhibitor, Resolving Gel Solution ,Transfer Buffer ,Blocking Buffer
Primary Antibody Aquaporin 5/AQP5 Antibody Picoband®
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody 1:10,000, HRP-conjugated Goat Anti-Rabbit IgG
Secondary Incubation 1h at 37℃
Detection Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary AQP5 (Aquaporin 5) is a transmembrane water channel protein that primarily regulates cellular water transport and plays a critical and complex role in tumor progression. In breast cancer, high AQP5 expression is often associated with increased tumor invasiveness, lymph node metastasis, and poor patient prognosis. In this experiment, AQP5 protein levels were markedly increased in cells treated with the agonist, whereas they were significantly reduced in cells treated with the inhibitor.

The Anti-TRPV1 antibody (A00128-4) showed clear and correctly positioned bands in WB of MADB106 rat breast cancer cells, with increased TRPV1 expression upon agonist treatment and decreased expression upon inhibitor treatment.

Excellent, submitted by on
SKU A00128-4
Application Western blot
Sample Rat MADB106 breast cancer cells
Sample Processing Description Normally cultured MADB106 cells, MADB106 cells treated with an agonist, MADB106 cells treated with an inhibitor
Other ReagentsRIPA lysis buffer (Cat. No. AR0102, Bosterbio, Wuhan) Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody TRPV1 Antibody Picoband®
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody 1:10,000, HRP-conjugated Goat Anti-Rabbit IgG
Secondary Incubation 1h at 37℃
Detection Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary The role of TRPV1 in breast cancer research is highly complex, varying with cell type, tumor subtype, and tumor microenvironment, and in some cases even showing contradictory effects. The current consensus is that TRPV1 is not only a potential therapeutic target for breast cancer but also an important prognostic and regulatory factor. Experimental results show that TRPV1 protein expression markedly increases in cells treated with an agonist, while it decreases in cells treated with an inhibitor.

The Anti-c-FOS antibody (M00297) showed clear and specific immunofluorescence staining in mouse brain, with markedly increased c-Fos expression in neurons of the acute ischemic region compared to normal brain.

Excellent, submitted by on
SKU M00297
Application Immunocytochemistry
Sample mouse brain
Sample Processing Description Normal brain tissue and brain tissue from a cerebral infarction model
Other ReagentsGoat serum, DAB Chromogenic Solution, Anti-fade mounting medium
Primary Antibody c-Fos Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody DyLight 594 Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 45 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary c-Fos is a marker of activated neural cells, especially neurons. During the acute phase of cerebral infarction, as a strong stress and injury stimulus, it drives c-Fos expression in the brain—particularly in the peri-infarct region—far higher than in normal resting brain. The experimental results show clear staining of positive cells, and the expression levels are consistent with expectations.

The Anti-FAP antibody (M00422) showed clear and specific IHC staining in human hepatocellular carcinoma, with low expression in adjacent normal tissue and strong expression in tumor stroma.

Excellent, submitted by on
SKU M00422
Application Immunohistochemistry
Sample human liver cancer
Sample Processing Description Collected clinical surgical human hepatocellular carcinoma and adjacent peritumoral tissues were fixed in formalin and embedded in paraffin.
Other ReagentsGoat serum, DAB Chromogenic Solution
Primary Antibody FAP1 Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Two-step IHC kit (Immunohistochemistry kit
Secondary Incubation 30 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary FAP (fibroblast activation protein) expression is mainly associated with stromal cells in the tumor microenvironment and is very low in normal human tissues. Its role in hepatocellular carcinoma (HCC) is complex, but overall it functions as an important pro-tumor factor. High FAP expression is associated with poor prognosis. It can remodel the extracellular matrix, promote tumor invasion and metastasis, and suppress anti-tumor immunity. In this study, FAP showed low expression in peritumoral tissues (close to normal tissue) and high expression in the tumor stroma of HCC.