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A Good Drosophila Me31B Antibody for WB

--Svetlana Dzitoyeva, University of Colorado, Denver, Biochemistry and Molecular Genetics, Research Scientist

Excellent, submitted by on

Source: Biocompare.com

SKU DZ33938-1
Application Western Blot
Sample Insect, D. melanogaster
Detection ECL

"The research of our lab aims to understand the mechanisms of maternally deposited RNAs and proteins clearance during maternal-to-zygotic transition (MZT). This is a very good AB, tested on WB only. The image shows GFP-tagged Me31B. Very good and clean. Clean and sharp band. Recommend Boster antibody production service."

Verification of Antibody on Recombinant Protein

--Hanjo Hellmann, Washington State University, School of Biological Sciences, Associate Professor

Excellent, submitted by on

Source: Biocompare.com

SKU DZ33980
Application Western Blot
Sample Recombinant protein expressed in and purified from E. coli
Detection Chemiluminescence

"We tested the antibody against recombinant GST-tagged BnaA07g20720D protein. As controls, we used another GST-tagged protein GST-MYB25, and GST alone. The antibody was well capable of detecting specifically BnaA07g20720D. We loaded ~100 ng of either GST:BnaA07g20720D, GST:MYB25, or GST alone, and the antibody was very specifically detecting BnaA07g20720D. It did not cross-react at all with the other two proteins. Highly specific. I would highly recommend the antibody for basic western-blot analysis of purified proteins. Probably one can significantly reduce the dilution to 1:10000 to still get a good result. Unfortunately, we did not have the opportunity with good controls to verify how the antibody works on whole plant extracts or for IP experiments."

Good Zebrafish CDHR1a Antibody from Boster Bio

--Jakub Famulski, University of Kentucky, Biology, Principal Investigator

Excellent, submitted by on

Source: Biocompare.com

SKU DZ07988
Application Immunofluorescence
Sample Zebrafish embryos, 5 days old
Detection Confocal Microscopy

"The antibody clearly labelled the outer segments of photoreceptor cells. The antibody is clean and precise. A zebrafish specific antibody for cdhr1a that WORKS!"

Good Zebrafish-Specific Siah1 Antibody from Boster Bio

--Jakub Famulski, University of Kentucky, Biology, Principal Investigator

Excellent, submitted by on

Source: Biocompare.com

SKU DZ02095
Application Immunofluorescence
Sample Zebrafish embryos 5dpf
Detection Confocal Microscopy

"Antibody labels the retina outer segments and inner nuclear layers as expected based on in situ hybridization studies. The staining is clean. A zebrafish-specific antibody for Siah1 that actually WORKS!"

Good Zebrafish Antibody for Nlz2

--Jakub Famulski, University of Kentucky, Biology, Principal Investigator

Excellent, submitted by on

Source: Biocompare.com

SKU DZ12892
Application Immunofluorescence
Sample Zebrafish embryos 24hpf
Detection Confocal Microscopy

"Zebrafish-specific antibody. Used in whole mount IF on 24hpf zebrafish embryos. The signal is not precisely clean, but we do observe nlz2 expression in the ventral region of the retina as expected from in situ hybridization results. This antibody shows some promising results in whole mount applications using 24hpf embryos. Applications with cryosections may be more precise."