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| SKU | M30929 |
|---|---|
| Application | Western Blot |
| Sample | HEK293T |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Blocking Agent | 5% non-fat milk |
| Secondary Antibody | Anti-Rabbit IgG Secondary Antibody, HRP-conjugated |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Detection | Signal was developed using ECL substrate on a Azure Biosystems c600. |
| Results Summary | I will definitely purchase BosterBio products again and recommend them to my classmates and colleagues. |
| SKU | DZ01481 |
|---|---|
| Application | Immunofluorescence |
| Sample | wildtype and KO mutant zebrafish retina |
| Primary Incubation | 1:200 |
| Blocking Agent | 10% normal goat serum and 2% bovine serum albumin in PBS |
| Secondary Antibody | Alexa Fluor 568 goat anti-rabbit was used in blocking solution. |
| SKU | M03918 |
|---|---|
| Application | Western Blot |
| Sample | Mouse brown adipose tissue |
| Sample Processing Description | Tissue and cell proteins were extracted using RIPA buffer. After BCA quantification, 5× loading buffer was added, and the samples were boiled for 5 minutes for denaturation. |
| Primary Incubation | The membrane was incubated with the PLIN1 primary antibodies (1:1000) overnight at 4 °C. |
| Secondary Antibody | Goat Anti-Rabbit IgG Antibody (1:5000) |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Other Reagents used | Non-fat milk |
| Detection | Signal was developed using ECL substrate |
| Results Summary | This antibody shows excellent consistency and reproducibility across batches, ensuring reliable and stable experimental results. Its performance remains consistent across different experiments, providing strong technical support for our research. |
| SKU | M00220-1 |
|---|---|
| Application | Western Blot |
| Sample | Mouse lung cancer tissue |
| Sample Processing Description | Tissue samples were directly lysed in RIPA buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98 °C. Load 20 µL of protein sample per lane onto SDS-PAGE. |
| Primary Incubation | The membrane was incubated with the CD86 primary antibodies (1:2000) overnight at 4 °C. |
| Secondary Antibody | HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Other Reagents used | 5% non-fat milk |
| Detection | Signal was developed using ECL substrate on an Tanon system. |
| Results Summary | The antibody is highly efficient and specific, showing a clear target band with no nonspecific bands. I will definitely continue using BosterBio products and will recommend them to my classmates and colleagues. |
| SKU | M00101-1 |
|---|---|
| Application | Western Blot |
| Sample | Mouse pancreatic acinar cells |
| Sample Processing Description | After centrifugation, collect the supernatant. Take a small portion for protein quantification using the BCA assay. Mix the remaining protein solution with an equal volume of loading buffer and denature in a 100°C water bath for 5 mins. |
| Primary Incubation | The membrane was incubated with the IL-6 and IL-1β primary antibodies (1:1000) overnight at 4 °C. |
| Secondary Antibody | HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Other Reagents used | Protein-Free Blocking Buffer |
| Detection | Signal was developed using ECL substrate on a ChemiDoc MP system. |
| Results Summary | IL-6 and IL-1β levels in the pancreas are key for assessing the anti-inflammatory effect of this nanomedicine. Antibodies from two previous suppliers showed poor specificity, while BosterBio antibodies proved highly specific, potent, and cost-effective. In our subsequent experiments, our group continued to use BosterBio products, which proved to be highly reliable and provided solid support for the publication of several articles. |
| SKU | DZ4103 |
|---|---|
| Application | Immunofluorescence |
| Sample | wildtype and KO mutant zebrafish retina |
| Primary Incubation | 1:100 |
| Blocking Agent | 10% normal goat serum and 2% bovine serum albumin in PBS |
| Secondary Antibody | Alexa Fluor 488 goat anti-rabbit and was used in a 1:800 dilution. |
| SKU | DZ41032 |
|---|---|
| Application | Immunofluorescence |
| Sample | wildtype and KO mutant zebrafish retina |
| Primary Incubation | 1:100 |
| Blocking Agent | 10% normal goat serum and 2% bovine serum albumin in PBS |
| Secondary Antibody | Alexa Fluor 568 goat anti-rabbit was used in a 1:800 dilution. |
Source: Biocompare.com
| SKU | P01424-1 |
|---|---|
| Application | Flow Cytometry |
| Sample | B cells |
| Primary Incubation | 0.25 µl/10^6 cells 50min RT |
| Blocking Agent | FBS 5% |
| Secondary Incubation | F(ab')2-Donkey anti-Rabbit IgG (H+L), PE 0.1 µl/10^6 |
| Detection | Flow Cytometry |
| Results Summary | Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million cells with pLYN antibody (P01424-1) during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In red secondary antibody only and in blue primary anti-phospho lyn + secondary antibody. |
Source: Biocompare.com
| SKU | DZ41383 |
|---|---|
| Application | Immunofluorescence |
| Sample | Zebrafish WKM, IPEX and FFPE tissue sections |
| Primary Incubation | Overnight |
| Blocking Agent | 3% goat serum in PBS |
| Secondary Incubation | 1 hour |
| Tertiary Incubation | None |
| Detection | Fluorescence microscopy |
| DOI or PMID # | https://doi.org/10.1101/2024.04.29.591640 |
| Results Summary | Antibody detects eosinophils and stains eosinophil granula. It is highly specific and can be used in dilutions such as 1:1000 for IF. |
"We ordered this antibody as a custom antibody from Boster Bio to stain for zebrafish eosinophils in FFPE tissue sections and fixed cells. It works wonderfully: very bright, with no relevant background or off-target staining, and highly reproducible."
| SKU | DZ01398-1 |
|---|---|
| Application | Immunofluorescence |
| Sample | Zebrafish heart |
| Detection | Confocal Microscopy |
"Mef2a was detected in a cryosection of zebrafish heart using the rabbit anti-Mef2a antibody (cat# DZ01398-1, 1:200, 4 degrees overnight). AlexaFluor546 Conjugated Goat Anti-Rabbit IgG was used as a secondary antibody at 1:200 dilution and incubated for 1.5 h at room temperature."