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The antibody shows a clear target band at the correct position and has strong affinity for phosphorylated IRE1 protein from pig.

Excellent, submitted by on
SKU M00371
Application Western Blot
Sample Porcine intestinal tissue
Sample Processing Description Intestinal tissues from different segments of pigs infected with swine fever.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody ATF4 Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary ATF4 is a key regulator of the integrated stress response. When cells encounter conditions such as oxidative stress or endoplasmic reticulum stress, the expression of ATF4 is rapidly induced and upregulated. The aim of this experiment is to determine the expression levels of ATF4 protein in different intestinal segments infected with classical swine fever.

The antibody shows a clear target band at the correct molecular weight and exhibits strong affinity for phosphorylated EIF2A protein in pig samples.

Excellent, submitted by on
SKU P04387
Application Western Blot
Sample Porcine intestinal tissue
Sample Processing Description Intestinal tissues from different segments of pigs infected with swine fever.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody Anti-Phospho-EIF2S1 (S51) Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary Phosphorylation of eIF2α is one of the most important switches controlling intracellular protein synthesis. When cells encounter conditions such as oxidative stress or endoplasmic reticulum stress, phosphorylated eIF2α rapidly suppresses the majority of protein synthesis to help the cell cope with the crisis. The purpose of this experiment is to determine the expression levels of phosphorylated EIF2A protein in different intestinal segments infected with classical swine fever virus.

The antibody shows a clear target band, correct localization, minimal background, and overall good results.

Excellent, submitted by on
SKU P00003
Application Western Blot
Sample mouse 4T1 cells
Sample Processing Description normal 4T1 cells, LPS-stimulated 4T1 cells, cells treated with low, medium, or high doses of the drug, and cells treated with a positive control drug.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody
Primary Incubation 1:2500, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary With equal loading amounts, total MTOR protein levels were similar across all groups. However, phosphorylated MTOR levels showed significant changes: the blank group was the lowest, the model group the highest, and in the drug-treated groups, phosphorylation decreased sequentially from low, medium, to high dose. The positive control group was comparable to the high-dose group.

The antibody shows a clear target, correct localization, minimal background, and overall good results.

Excellent, submitted by on
SKU P00024-4
Application Western Blot
Sample mouse 4T1 cells
Sample Processing Description normal 4T1 cells, LPS-stimulated 4T1 cells, cells treated with low, medium, or high doses of the drug, and cells treated with a positive control drug.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody Anti-AKT1,2,3 Antibody Picoband®
Primary Incubation 1:10000, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary Under equal loading conditions, the total AKT protein levels were similar across all groups, while the amount of phosphorylated AKT1 showed significant changes. The blank group had the lowest level, the model group had the highest, and in the drug-treated groups, the levels decreased progressively from low to medium to high dose. The positive control drug group was close to the high-dose group.

The antibody shows a clear target, correct localization, minimal background, and overall good results.

Excellent, submitted by on
SKU M00003
Application Western Blot
Sample mouse 4T1 cells
Sample Processing Description normal 4T1 cells, LPS-stimulated 4T1 cells, cells treated with low, medium, or high doses of the drug, and cells treated with a positive control drug.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody Anti-ATF4 Rabbit Monoclonal Antibody
Primary Incubation 1:10000, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary With equal loading amounts, the total MTOR protein levels were similar across all groups. However, the levels of phosphorylated MTOR showed significant changes: the blank group had the lowest, the model group the highest, and in the drug-treated groups, the phosphorylation levels decreased sequentially from low, medium, to high dose. The positive control group was comparable to the high-dose group.

Our lab will continue to use this antibody, and we will recommend it to other researchers at the university working on similar studies.

Excellent, submitted by on
SKU A31732-2
Application Immunofluorescence
Sample Normal rat liver and alcoholic rat liver
Sample Processing Description The samples consist of myocardium from normal SD rats and myocardium from SD rats subjected to an alcoholic liver disease model. The alcoholic liver disease model was established by gavaging 50% ethanol twice daily, while allowing the rats to freely consume alcoholic beverages, for a total of 14 weeks.
Other Reagents Goat Serum, DAPI, Anti-fade mounting medium
Primary Antibody Anti-PTRF/CAVIN1 Antibody Picoband®
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody DyLight 488 Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:500, 45 minutes in room temperature
Detection Imaging system:Leica DM2500
Results Summary PTRF/CAVIN1 is a multifunctional protein that shuttles between the plasma membrane and the nucleus, assisting in the recruitment of repair proteins when the plasma membrane is damaged. The results of this IF experiment indicate that alcohol gavage induces myocardial injury and leads to increased expression of PTRF.

Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio and boiled for 10 minutes. Fifteen microliters of

Excellent, submitted by on
SKU M00003
Application Western Blot
Sample human OCI-LY1 cell
Sample Processing Description Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio and boiled for 10 minutes. Fifteen microliters of each sample were loaded per well.
Other Reagents 5% Non-fat milk
Primary Antibody mTOR/Tor Rabbit Monoclonal Antibody
Primary Incubation 1:3000, overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL reagent, Imaging system:ChemiDoc MP
Results Summary This antibody is highly specific and efficient, with a clean background and no nonspecific bands. The target band has sharp and well-defined edges.

This antibody is highly specific and efficient, with a clean background and no nonspecific bands. The target band has well-defined edges.

Excellent, submitted by on
SKU P00003
Application Western Blot
Sample human OCI-LY1 cell
Sample Processing Description Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio and boiled for 10 minutes. Fifteen microliters of each sample were loaded per well.
Other Reagents 5% Non-fat milk
Primary Antibody Phospho-mTOR (S2448) Rabbit Monoclonal Antibody
Primary Incubation 1:3000, overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL reagent, Imaging system:ChemiDoc MP
Results Summary This antibody is highly specific and efficient, with a clean background and no nonspecific bands. The target band has sharp and well-defined edges.

This antibody is highly specific and efficient, with a clear target band and no nonspecific bands.

Excellent, submitted by on
SKU M00220-1
Application Western Blot
Sample mouse lung cancer tissue
Sample Processing Description Tissue samples were directly lysed in RIPA buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98°C. Twenty microliters of each protein sample were loaded per lane onto an SDS-PAGE gel.
Other Reagents 5% Non-fat milk
Primary Antibody CD86/B7 2 Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL reagent, Imaging system:Tanon
Results Summary This antibody is highly specific and efficient, with a clear target band and no nonspecific bands.

The Anti-Cytokeratin antibody (M02416-2) produced clear and specific target bands in WB analysis of untreated and drug-treated HTR8 cells, demonstrating reliable and consistent detection without non-specific signals.

Excellent, submitted by on
SKU M02416-2
Application Western Blot
Sample human HTR8 cells
Sample Processing Description Cells were lysed with RIPA lysis buffer, mixed with β-mercaptoethanol, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents blocking buffer
Primary Antibody Cytokeratin 7 KRT7 Rabbit Monoclonal Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL reagent, Imaging system:ChemiDoc MP
Results Summary The figure shows WB results of Cytokeratin in untreated and drug-treated HTR8 cells, with clear and distinct bands at the expected position, demonstrating reliable and specific detection.