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The CD71 antibody was used to detect protein expression in mouse uterine tissue. The bands were clear with no nonspecific signals. After recovery and reuse, the antibody still performed well.

Excellent, submitted by on
SKU PB9233
Application Western Blot
Sample mouse uterine tissue
Sample Processing Description Mouse uterine tissues were minced and further disrupted by sonication, then lysed on ice for 1 hour using RIPA buffer. After centrifugation, the supernatant was collected and quantified using the BCA method. The appropriate amount of loading buffer was added, and the samples were boiled in a water bath to denature the proteins. Finally, 15 μL of each protein sample was loaded into each lane of the SDS-PAGE gel.
Other Reagents 5% Non-fat milk
Primary Antibody Transferrin Receptor/TFRC Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL reagent, Imaging system:Tanon
Results Summary The CD71 antibody was used to detect protein expression in mouse uterine tissue. The bands were clear with no nonspecific signals, and after recovery and reuse, the antibody still showed good performance.

This antibody is highly specific and efficient, with a clean background and no nonspecific bands. The target band has well-defined edges.

Excellent, submitted by on
SKU P00003
Application Western Blot
Sample human OCI-LY1 cell
Sample Processing Description Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio and boiled for 10 minutes. Fifteen microliters of each sample were loaded per well.
Other Reagents 5% Non-fat milk
Primary Antibody Phospho-mTOR (S2448) Rabbit Monoclonal Antibody
Primary Incubation 1:3000, overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL reagent, Imaging system:ChemiDoc MP
Results Summary This antibody is highly specific and efficient, with a clean background and no nonspecific bands. The target band has sharp and well-defined edges.

This antibody is highly specific and efficient, with a clear target band and no nonspecific bands.

Excellent, submitted by on
SKU M00220-1
Application Western Blot
Sample mouse lung cancer tissue
Sample Processing Description Tissue samples were directly lysed in RIPA buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98°C. Twenty microliters of each protein sample were loaded per lane onto an SDS-PAGE gel.
Other Reagents 5% Non-fat milk
Primary Antibody CD86/B7 2 Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL reagent, Imaging system:Tanon
Results Summary This antibody is highly specific and efficient, with a clear target band and no nonspecific bands.

This antibody is highly specific and efficient, suitable for detecting Vimentin protein in HTR8 cells by Western blot. Only minor nonspecific bands are observed.

Excellent, submitted by on
SKU PB9359
Application Western Blot
Sample human HTR8 cell
Sample Processing Description The samples were lysed in RIPA buffer, mixed with β-mercaptoethanol, and denatured at 100°C for 10 minutes before loading onto an SDS-PAGE gel.
Other Reagents 5% Non-fat milk
Primary Antibody Vimentin Antibody Picoband®
Primary Incubation 1:2500, overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL reagent, Imaging system:ChemiDoc MP
Results Summary This antibody is highly specific and efficient, suitable for detecting Vimentin protein in HTR8 cells by Western blot, with only minor nonspecific bands observed.

This antibody is highly specific and efficient, suitable for detecting BIK in tumor tissues.

Excellent, submitted by on
SKU PB9755
Application Immunohistochemistry
Sample human lung cancer tissue
Sample Processing Description Paraffin-embedded tumor tissue sections were dewaxed with graded xylene and alcohol, followed by antigen retrieval in EDTA buffer for 10 minutes. The sections were then blocked with goat serum for 30 minutes.
Other Reagents EDTA antigen retrieval buffer
Primary Antibody Bik Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Imaging system:Olympus
Results Summary This antibody is highly specific and efficient, suitable for detecting BIK in tumor tissues.

The Anti-Cytokeratin antibody (M02416-2) produced clear and specific target bands in WB analysis of untreated and drug-treated HTR8 cells, demonstrating reliable and consistent detection without non-specific signals.

Excellent, submitted by on
SKU M02416-2
Application Western Blot
Sample human HTR8 cells
Sample Processing Description Cells were lysed with RIPA lysis buffer, mixed with β-mercaptoethanol, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents blocking buffer
Primary Antibody Cytokeratin 7 KRT7 Rabbit Monoclonal Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL reagent, Imaging system:ChemiDoc MP
Results Summary The figure shows WB results of Cytokeratin in untreated and drug-treated HTR8 cells, with clear and distinct bands at the expected position, demonstrating reliable and specific detection.

This antibody is suitable for detecting PTEN protein in mouse hippocampus by Western blot, showing clear, distinct, and highly specific bands.

Excellent, submitted by on
SKU M00006-1
Application Western Blot
Sample Mouse hippocampus tissue
Sample Processing Description The mouse hippocampus was lysed with RIPA buffer containing a protease inhibitor cocktail. After protein quantification, samples were mixed with 5× protein loading buffer and heated for 10 minutes to denature. Load 5 μL of protein per lane and apply to SDS-PAGE.
Other Reagents 5% Non-fat milk
Primary Antibody Anti-PTEN Rabbit Monoclonal Antibody
Primary Incubation overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: Ultra-sensitive ECL luminescent reagent, Imaging system:ChemiDoc MP
Results Summary This antibody is suitable for detecting PTEN protein in mouse hippocampus by Western blot, showing clear, distinct, and highly specific bands.

The GPX4 antibody was used to detect the expression of the protein in mouse uterine tissue. The WB results showed clear bands, and the antibody could be reused after recovery with good performance, offering excellent cost-effectiveness.

Excellent, submitted by on
SKU M02059
Application Western Blot
Sample Mouse Uterus tissue
Sample Processing Description The GPX4 antibody was used to detect the expression of the protein in mouse uterine tissue. The Western blot results showed clear bands, and the antibody retained good performance after reuse, offering excellent cost-effectiveness.
Primary Antibody Anti-GPX4 Rabbit Monoclonal Antibody
Primary Incubation overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system:Tanon
The GPX4 antibody was used to detect the protein expression in mouse uterine tissue. The Western blot results showed clear bands, and the antibody maintained good performance after reuse, providing excellent cost-effectiveness.

The FTH1 antibody was used to detect the expression of the target protein in human uterine tissue. The WB bands were single and clear, and compared with other domestic and international brands, this antibody offers excellent cost-performance.

Excellent, submitted by on
SKU M02401
Application Western Blot
Sample Mouse hippocampus tissue
Sample Processing Description The tissue was minced and sonicated, then lysed on ice for 1 hour using RIPA buffer. After centrifugation to collect the supernatant and protein quantification by BCA, samples were mixed with loading buffer at the appropriate ratio and denatured by boiling in a water bath. Fifteen microliters of each protein sample were loaded per lane onto SDS-PAGE gel.
Primary Antibody Anti-Ferritin FTH1 Rabbit Monoclonal Antibody
Primary Incubation overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system:Tanon
Results Summary The FTH1 antibody was used to detect the expression of the target protein in human uterine tissue. The WB bands were single and clear, and compared with other domestic and international brands, this antibody offers excellent cost-performance.

Western blot analysis was performed using the COL6A1 antibody to detect COL6A1 protein expression in the mouse hippocampus.

Excellent, submitted by on
SKU M02226
Application Western Blot
Sample Mouse hippocampus tissue
Sample Processing Description The mouse hippocampus was lysed in RIPA buffer supplemented with a protease inhibitor cocktail. After protein quantification, samples were mixed with 5× protein loading buffer and denatured by heating at 100°C for 10 minutes. Five microliters of each protein sample were loaded per lane onto SDS-PAGE.
Primary Antibody Anti-Collagen VI COL6A1 Rabbit Monoclonal Antibody
Primary Incubation overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system:ChemiDoc MP
Results Summary Western blot analysis was performed using the COL6A1 antibody to detect COL6A1 protein expression in the mouse hippocampus. Although minor non-specific bands were observed, they did not affect the trend analysis, indicating that the antibody is suitable for detecting the target protein in this tissue.