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The CTL4 antibody was efficiently used to detect CTL4 protein secreted in the mosquito hemolymph. The protein migrates at ~15 kDa in SDS-PAGE.

Excellent, submitted by on
SKU DZ41074
Application Western Blot
Sample β-galactosidase gene knockdown mosquito hemolymph, CTL4 knockdown mosquito hemolymph
Primary Incubation +4°C overnight
Secondary Incubation For 1 hour at room temperature
Tertiary Incubation 1:1000
Detection Clarity Max western ECL substrate
Results Summary Image showing the specificity of CTL4 antibody. Lane 1 includes hemolymph extracted from mosquitoes silenced for the bacterial β-galactosidase gene. Lane 2 contains hemolymph extracted from mosquitoes silenced for the CTL4 gene.

The CLIPA14 antibody was efficiently used to detect CLIPA14 protein secreted in the mosquito hemolymph. The protein migrates at ~59 kDa in SDS-PAGE.

Excellent, submitted by on
SKU DZ41073
Application Western Blot
Sample β-galactosidase gene knockdown mosquito hemolymph, CLIPA14 knockdown mosquito hemolymph
Primary Incubation +4°C overnight
Secondary Incubation For 1 hour at room temperature
Tertiary Incubation 1:3000
Detection Clarity Max western ECL substrate
Results Summary Image showing the specificity of CLIPA14 antibody. Lane 1 includes hemolymph extracted from mosquitoes silenced for the bacterial β-galactosidase gene. Lane 2 contains hemolymph extracted from mosquitoes silenced for the CLIPA14 gene.

High-Quality Serinc5 Antibody From Boster Bio Produces a Clear Band

Excellent, submitted by on

Source: Biocompare.com

SKU A06603
Application Western Blot
Sample Ripa buffer lysis transfected HEK293 cell
Primary Incubation +4°C overnight
Blocking Agent 5% milk in 1X TBST
Secondary Incubation 30 mins
Tertiary Incubation 1:1000
Detection Clarity Western ECL Substrate
Results Summary The antibody is excellent at recognizing and expressing the protein at the correct position. I have tried samples from different vendors, but only this vendor offers high-quality products.

Both Are Confirmed Specific In Zebrafish Using One Of Our KO Lines.

Excellent, submitted by on
SKU DZ4103
Application Immunofluorescence
Sample wildtype and KO mutant zebrafish retina
Primary Incubation 1:100
Blocking Agent 10% normal goat serum and 2% bovine serum albumin in PBS
Secondary Antibody Alexa Fluor 488 goat anti-rabbit and was used in a 1:800 dilution.

Both are confirmed specific in zebrafish using one of our KO lines.

Excellent, submitted by on
SKU DZ41032
Application Immunofluorescence
Sample wildtype and KO mutant zebrafish retina
Primary Incubation 1:100
Blocking Agent 10% normal goat serum and 2% bovine serum albumin in PBS
Secondary Antibody Alexa Fluor 568 goat anti-rabbit was used in a 1:800 dilution.

Anti-phospho Lyn (Y396) Rabbit Monoclonal Antibody

Excellent, submitted by on

Source: Biocompare.com

SKU P01424-1
Application Flow Cytometry
Sample B cells
Primary Incubation 0.25 µl/10^6 cells 50min RT
Blocking Agent FBS 5%
Secondary Incubation F(ab')2-Donkey anti-Rabbit IgG (H+L), PE 0.1 µl/10^6
Detection Flow Cytometry
Results Summary Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million cells with pLYN antibody (P01424-1) during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In red secondary antibody only and in blue primary anti-phospho lyn + secondary antibody.

Mettl23 Detection in Cell Lines

Excellent, submitted by on

Source: Biocompare.com

SKU DZ41142
Application Western Blot
Sample Human cell lines
Primary Incubation 1:500 with 1% BSA in TBS, incubated overnight, at 4oC, with shaking
Blocking Agent 5% BSA in TBS
Secondary Incubation Licor IRDye 800 Goat anti Rabbit diluted 1:10000 with 1% BSA in TBS, incubated for 1 hour at RT , with shaking
Detection Licor Odyssey IR Scanner
Additional Notes The antibody has very good stability at room temperature (transit took over 2 weeks and the ice packs had long melted before delivery).
Results Summary The antibody gave a detectable band at the expected size for all cell lines tested with no non-specific bands.

"This antibody was used to determine the level of protein expression in various cell lines in order to set up two groups (high vs low expression). This antibody was custom made because numerous suppliers have either stopped their production for this antibody or quality/specificity was low."

Beautiful Imaging of Zebrafish Eosinophils

Excellent, submitted by on

Source: Biocompare.com

SKU DZ41383
Application Immunofluorescence
Sample Zebrafish WKM, IPEX and FFPE tissue sections
Primary Incubation Overnight
Blocking Agent 3% goat serum in PBS
Secondary Incubation 1 hour
Tertiary Incubation None
Detection Fluorescence microscopy
DOI or PMID # https://doi.org/10.1101/2024.04.29.591640
Results Summary Antibody detects eosinophils and stains eosinophil granula. It is highly specific and can be used in dilutions such as 1:1000 for IF.

"We ordered this antibody as a custom antibody from Boster Bio to stain for zebrafish eosinophils in FFPE tissue sections and fixed cells. It works wonderfully: very bright, with no relevant background or off-target staining, and highly reproducible."

"Works well for immunofluorescence"

Excellent, submitted by on

Source: Customer Feedback Submission

"Works well for immunofluorescence"

--Jason Tennessen

SKU DZ41223
Application IF
Sample Drosophila larval fat body

"We’ve verified the specificity of this custom antibody that Boster generated for us. The antibody "Anti-Fruit Fly Gpdh1 Antibody (DZ41223)” works well for immunofluorescence. A 1:100 dilution of this antibody stains fat body tissues of wild-type larvae but not Gpdh1 mutant. I’m happy to advertise this antibody to the fly community."

Western blot for Polyclonal Anti-Drosophila melanogaster (Fruit fly) emp Antibody

--Yuzuru Imai, Department of Research for Parkinson's Disease Juntendo University Graduate School of Medicine

Excellent, submitted by on
SKU DZ41152
Application Western blot
Sample Drosophila melanogaster (head and thorax)
Detection Immobilon Forte Western HRP substrate

"There are non-specific bands and immunostaining may not be possible, but Western blotting is sufficient with this antibody."