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| SKU | M30929 |
|---|---|
| Application | Western Blot |
| Sample | HEK293T |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Blocking Agent | 5% non-fat milk |
| Secondary Antibody | Anti-Rabbit IgG Secondary Antibody, HRP-conjugated |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Detection | Signal was developed using ECL substrate on a Azure Biosystems c600. |
| Results Summary | I will definitely purchase BosterBio products again and recommend them to my classmates and colleagues. |
| SKU | PA1079 |
|---|---|
| Application | Western Blot |
| Sample | Mouse lung tissue |
| Sample Processing Description | Tissue samples were directly lysed in RIPA buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98 °C. Load 20 µL of protein sample per lane onto SDS-PAGE. |
| Primary Incubation | The membrane was incubated with TNF-α primary antibodies (1:1000) overnight at 4 °C. |
| Secondary Antibody | HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Other Reagents used | 5% Non-fat milk |
| Detection | Signal was developed using ECL substrate on an ChemiDoc MP system. |
| Results Summary | This antibody shows excellent consistency and reproducibility across batches, ensuring reliable and stable experimental results. Its performance remains consistent across different experiments, providing strong technical support for our research. |
| SKU | M03918 |
|---|---|
| Application | Western Blot |
| Sample | Mouse brown adipose tissue |
| Sample Processing Description | Tissue and cell proteins were extracted using RIPA buffer. After BCA quantification, 5× loading buffer was added, and the samples were boiled for 5 minutes for denaturation. |
| Primary Incubation | The membrane was incubated with the PLIN1 primary antibodies (1:1000) overnight at 4 °C. |
| Secondary Antibody | Goat Anti-Rabbit IgG Antibody (1:5000) |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Other Reagents used | Non-fat milk |
| Detection | Signal was developed using ECL substrate |
| Results Summary | This antibody shows excellent consistency and reproducibility across batches, ensuring reliable and stable experimental results. Its performance remains consistent across different experiments, providing strong technical support for our research. |
| SKU | M00220-1 |
|---|---|
| Application | Western Blot |
| Sample | Mouse lung cancer tissue |
| Sample Processing Description | Tissue samples were directly lysed in RIPA buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98 °C. Load 20 µL of protein sample per lane onto SDS-PAGE. |
| Primary Incubation | The membrane was incubated with the CD86 primary antibodies (1:2000) overnight at 4 °C. |
| Secondary Antibody | HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Other Reagents used | 5% non-fat milk |
| Detection | Signal was developed using ECL substrate on an Tanon system. |
| Results Summary | The antibody is highly efficient and specific, showing a clear target band with no nonspecific bands. I will definitely continue using BosterBio products and will recommend them to my classmates and colleagues. |
| SKU | M00101-1 |
|---|---|
| Application | Western Blot |
| Sample | Mouse pancreatic acinar cells |
| Sample Processing Description | After centrifugation, collect the supernatant. Take a small portion for protein quantification using the BCA assay. Mix the remaining protein solution with an equal volume of loading buffer and denature in a 100°C water bath for 5 mins. |
| Primary Incubation | The membrane was incubated with the IL-6 and IL-1β primary antibodies (1:1000) overnight at 4 °C. |
| Secondary Antibody | HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Other Reagents used | Protein-Free Blocking Buffer |
| Detection | Signal was developed using ECL substrate on a ChemiDoc MP system. |
| Results Summary | IL-6 and IL-1β levels in the pancreas are key for assessing the anti-inflammatory effect of this nanomedicine. Antibodies from two previous suppliers showed poor specificity, while BosterBio antibodies proved highly specific, potent, and cost-effective. In our subsequent experiments, our group continued to use BosterBio products, which proved to be highly reliable and provided solid support for the publication of several articles. |
| SKU | PB9148 |
|---|---|
| Application | Western Blot |
| Sample | hemolymph of the Pacific oyster |
| Sample Processing Description | Add 10 µL of hemolymph cells to each lane and directly lyse in 1× Laemmli sample buffer. After adding β-mercaptoethanol, load the samples onto SDS-PAGE. |
| Primary Incubation | The membrane was incubated with the MyD88 primary antibody (1:1000) overnight at 4 °C. |
| Secondary Antibody | HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Other Reagents used | Milk |
| Detection | Signal was developed using ECL substrate on a ChemiDoc MP system. |
| Results Summary | The antibody shows high efficiency and specificity in Western blot detection of hemolymph cell proteins from Pacific oyster, with only slight nonspecific bands. |
Source: Biocompare.com
| SKU | PA1021-2 |
|---|---|
| Application | Western Blot |
| Sample | Tumor cell lysate |
| Primary Incubation | Overnight at 8-10 degrees Celcius, with rocking, 1 ug/ml in 5% milk/BPS/Tw |
| Blocking Agent | 5% milk in PBS/0.05% Tween-20 (5% milk/PBS/Tw) |
| Secondary Incubation | Goat anti-Rabbit antibody conjugated with HRP at 1:3,000 in 5% milk/PBS/Tw |
| Tertiary Incubation | HRP-bound secondary antibodies were detected by WestPico from ThermoScientific/Tw |
| Detection | Chemiluminescence: West Pico from Thermo Scientific |
| Results Summary | The antibody recognizes the expected ~80 kDa full-length CD44 and its low mol. wt. fragments containing C-terminal domain. The antibody is highly specific, produces "clean", definitive results; does not produce any non-specific bands. The antibody is sensitive and detects CD44 band when total protein per lane is loaded at 10-20 ug. The antibody is stable and could be re-used for blotting several times when stored in the original 5% milk/PBS/Tw solution at -20℃. |
"The antibody was used to detect the full length and cleaved fragments of human transmembrane protein CD44. The rabbit antibody PA1021-2 is sensitive, i.e. detects CD44 protein bands under reducing conditions and also when tested material is loaded at low total protein per lane. The antibody is highly specific, i.e. does not recognize any bands of unknown nature on the membrane."
Source: Biocompare.com
| SKU | PA1667 |
|---|---|
| Application | Western Blot |
| Sample | 22RV1 cytosolic and membrane fractions |
| Primary Incubation | 1:1000, overnight at 4 degree |
| Blocking Agent | 5% Milk |
| Secondary Incubation | 1:5000, 1 hour RT. |
| Detection | ECL |
| Results Summary | Please refer our paper (Scientific Reports 6, Article number: 23135 (2016)doi:10.1038/srep23135). |
"We used this antibody to study the inhibitory effect of GPE on hypoxia induced translocation of gp91phox subunit from cytosol to membrane in our recently published paper (Scientific Reports 6, Article number: 23135 (2016); doi:10.1038/srep23135). This antibody is very much specific in our in vitro system."
Source: Biocompare.com
| SKU | P01424-1 |
|---|---|
| Application | Flow Cytometry |
| Sample | B cells |
| Primary Incubation | 0.25 µl/10^6 cells 50min RT |
| Blocking Agent | FBS 5% |
| Secondary Incubation | F(ab')2-Donkey anti-Rabbit IgG (H+L), PE 0.1 µl/10^6 |
| Detection | Flow Cytometry |
| Results Summary | Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million cells with pLYN antibody (P01424-1) during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In red secondary antibody only and in blue primary anti-phospho lyn + secondary antibody. |
Source: Biocompare.com
| SKU | A05219 |
|---|---|
| Application | Western Blot |
| Sample | HEK293T |
| Primary Incubation | 1:5000 for 24hours at 4degrees celsius. |
| Blocking Agent | Skim milk |
| Secondary Incubation | 1 hour room temperature 1:3000 |
| Detection | ECL |
| Results Summary | We silenced ULK1 and ULK2 genes in HEK293T cells, the antibody could differentiate the two proteins. |
"Used for western blotting. The antibody worked good, used skim milk for blocking. It was used for investigating silenced HEK293T cells."