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Immunofluorescence using Anti-GFAP antibody (MA1045) clearly labeled protoplasmic astrocytes in the spinal cord gray matter, showing dense, bushy processes surrounding neurons, with excellent specificity and staining.

Excellent, submitted by on
SKU MA1045
Application Immunofluorescence
Sample rat spinal tissue
Sample Processing Description Paraffin-embedded transverse sections of rat spinal cord were prepared after formalin fixation.
Other ReagentsTris-EDTA Antigen Retrieval Buffer (50×, pH 9.0), DAPI
Primary Antibody GFAP Antibody (Monoclonal, G-A-5)
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Goat Anti-Mouse IgG (H+L) Secondary Antibody, Fluoro488 Conjugated
Secondary Incubation 45 minutes in 37℃
Detection Imaging system:Leica DM2500
Results Summary GFAP is a marker of astrocytes. In this experiment, immunostaining for GFAP was used to label astrocytes in the gray matter of the spinal cord to observe their distribution, density, and morphology. The results showed that the labeled protoplasmic astrocytes in the gray matter had short, thick, and highly branched processes with rough surfaces, forming a dense “bushy” network tightly surrounding neuronal cell bodies and synapses, consistent with theoretical expectations and demonstrating excellent staining.

This antibody is highly efficient and specific, suitable for detecting AQP4 protein in rat colon by Western blot, with only minimal nonspecific bands.

Excellent, submitted by on
SKU PB9475
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody Aquaporin 4/AQP4 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows a schematic representation of Western blot results for the target protein AQP4 and the loading control Actin in rat colon across the normal, model, traditional Chinese medicine, and western medicine groups. The target bands are clear and distinct, and the experimental results are satisfactory.

BOSTER’s rabbit anti-MBP antibody (catalog BA0094) exhibits high specificity and low background, enabling sensitive detection of demyelination and myelin regeneration processes, greatly facilitating this study.

Excellent, submitted by on
SKU PA1050
Application Immunofluorenscence
Sample Mouse spinal cord
Sample Processing Description Mouse spinal cord fixed with 2% paraformaldehyde for 6–8 hours, dehydrated in 30% sucrose, embedded in OCT, and sectioned using a cryostat.
Primary Antibody Anti-MBP9 antibody
Primary Incubation 1:100, overnight at 4 ℃
Blocking Agent 3% BSA
Secondary Antibody Anti-rabbit IgG-CY3 conjugated antibody.
Secondary Incubation Incubate at room temperature for 1 hour
Detection Immunofluorescence images were acquired using a confocal laser microscope (Leica SP8, Zeiss LSM 880 Airyscan).
Results Summary BOSTER’s rabbit anti-MBP antibody (catalog BA0094) has high specificity and low background, enabling sensitive detection of demyelination and myelin regeneration processes, greatly facilitating this study.